Abstract: A clean connection and sampling apparatus (10) and method for creating a controlled environment for the transfer of chemicals has an isolation means for providing an isolated area (14) capable of being cleaned. A first connection means (16) for connection to a supply of chemicals to be transferred is provided and a second connection means (18) for transferring the chemicals from the isolation means to a receiver for said chemicals is also provided. A sampling means is located within the isolation means for sampling the chemical prior to transfer to the receiver. A pass-through means (22) is provided for receiving a sample from within the isolation means and isolating the sample from the isolation means prior to transfer of the sample outside of the isolation means for analysis.