Abstract: The present invention provides a luminogenic, in particular a phosphorogenic transition metal-based pyridyl complex containing a nitrone moiety, which nitrone moiety acts as a bioorthogonal functional group and an emission quencher, and can undergo cycloaddition reaction with a complementary bioorthogonal functional group coupled to a substrate. The transition metal is can be selected from iridium or ruthenium. Also disclosed is a method for preparing the transition metal-based pyridyl complex and a pharmaceutical composition comprising it. Still further provided is a method for bioorthogonal labeling of a biomolecule, a method for staining of a cell structure, a method for in vivo imaging of an organism, and a kit for in vivo imaging of an organism. The luminogenic properties and high reactivity of the complexes are highly advantageous for bioorthogonal labeling and imaging of biomolecules in their native biological environments at much lower costs than those of the existing commercial products.

Abstract: The present invention provides a luminogenic, in particular a phosphorogenic transition metal-based pyridyl complex containing a nitrone moiety, which nitrone moiety acts as a bioorthogonal functional group and an emission quencher, and can undergo cycloaddition reaction with a complementary bioorthogonal functional group coupled to a substrate. The transition metal is can be selected from iridium or ruthenium. Also disclosed is a method for preparing the transition metal-based pyridyl complex and a pharmaceutical composition comprising it. Still further provided is a method for bioorthogonal labeling of a biomolecule, a method for staining of a cell structure, a method for in vivo imaging of an organism, and a kit for in vivo imaging of an organism. The luminogenic properties and high reactivity of the complexes are highly advantageous for bioorthogonal labeling and imaging of biomolecules in their native biological environments at much lower costs than those of the existing commercial products.

Abstract: A phosphorescent transition metal complex especially suitable as sensor for hydrogen peroxide in cells, in particular in mitochondria in live cells, includes a metallic central atom, which is a transition metal, a first ligand with at least one pyridine ring, and a second ligand having at least one pyridine ring and at least one structural component selected from the group of 1,2-diketone moiety and an arylboronate moiety. A method for preparing the metal complex is also disclosed as well as a method of detecting hydrogen peroxide in cells by incubating cells with the transition metal complex. The metal complex shows advantageous intense and long-lived phosphorescence accompanied by exceptional cellular localization properties. The metal complex is highly photostable and highly selective towards hydrogen peroxide, thus, especially suitable for detecting mitochondrial H2O2.

Abstract: A phosphorescent transition metal complex especially suitable as sensor for hydrogen peroxide in cells, in particular in mitochondria in live cells, includes a metallic central atom, which is a transition metal, a first ligand with at least one pyridine ring, and a second ligand having at least one pyridine ring and at least one structural component selected from the group of 1,2-diketone moiety and an arylboronate moiety. A method for preparing the metal complex is also disclosed as well as a method of detecting hydrogen peroxide in cells by incubating cells with the transition metal complex. The metal complex shows advantageous intense and long-lived phosphorescence accompanied by exceptional cellular localization properties. The metal complex is highly photostable and highly selective towards hydrogen peroxide, thus, especially suitable for detecting mitochondrial H2O2.

Abstract: The present invention is concerned with a staining dye for staining a biological cell, comprising dye molecules with a positively charged polar head group for attraction to cell membrane of the cell and for refraining the dye molecules from entering the cytoplasm, and one or more hydrophobic groups for interaction with phospholipids of the cell membrane. The dye molecules are phosphorescent transition metal polypyridine complexes having a metal center and ligands which are non-organic fluorophores.

Abstract: The present invention is concerned with a staining dye for staining a biological cell, comprising dye molecules with a positively charged polar head group for attraction to cell membrane of the cell and for refraining the dye molecules from entering the cytoplasm, and one or more hydrophobic groups for interaction with phospholipids of the cell membrane. The dye molecules are phosphorescent transition metal polypyridine complexes having a metal center and ligands which are non-organic fluorophores.

Abstract: A system and method for cross-carrier mobile device capability discovery. According to an embodiment, a content delivery platform receives an identifier associated with a mobile computing device of a user, identifies a wireless carrier associated with the mobile computing device based on the received identifier, and detects one or more media capabilities supported by the user's mobile computing device for delivering content to the user's mobile computing device based at least in part on media capabilities supported by the identified carrier.

Abstract: A system and method for managing distribution of multi-formatted content. According to an embodiment, a content aggregation platform provides a user interface to receive a location of content to be provided to the content aggregation platform by a content provider in a source format native to the content provider, and transformation instructions describing how to transform the source format of the content into a platform format native to the content aggregation platform, receives the content location and the transformation instructions via the user interface, and stores the content location and the transformation instructions in a database to support subsequent delivery of the content to one or more users for display.

Abstract: A system for facilitating access by a user of a vehicle dealership to messages related to a lead for a vehicle from a customer, the lead having a unique identifier, customer information of the customer including contact information, and requested vehicle information, the system comprising: a storage containing a plurality of data suitable for including in a response message to the customer; a data search module configured for searching the storage to identify response data from the plurality of data based on at least one of the customer information or the vehicle information; a messaging center module configured for receiving a customer message associated with the lead via the unique identifier; and a template library configured for providing access to a plurality of message templates, the message templates for use with the response data through data population of corresponding template fields in generation of a response message to the received customer message, the response message being associated with the

Abstract: A system for distributing a plurality of leads for vehicles over a network from a plurality of disparate lead sources to a targeted lead destination, the system comprising: an import module configured for receiving the plurality of leads in an electronic format and for associating a unique identifier with each lead of the plurality of leads, each of the leads including customer contact information, customer location information, and vehicle information; a tracking module configured for creating a report record for each said lead for use in tracking a status of each said lead, the report record associated with the unique identifier of each said lead; a delivery module configured for assigning each said lead to a selected vehicle dealership based on the lead information, the vehicle dealership associated with a network address of the targeted lead destination and for communicating each said lead to the network address; and a collector module configured for receiving from the targeted lead destination respective

Abstract: A system and method for cross-carrier mobile device capability discovery. According to an embodiment, a content delivery platform receives an identifier associated with a mobile computing device of a user, identifies a wireless carrier associated with the mobile computing device based on the received identifier, and detects one or more media capabilities supported by the user's mobile computing device for delivering content to the user's mobile computing device based at least in part on media capabilities supported by the identified carrier.

Abstract: A system and method for managing distribution of multi-formatted content. According to an embodiment, a content aggregation platform provides a user interface to receive a location of content to be provided to the content aggregation platform by a content provider in a source format native to the content provider, and transformation instructions describing how to transform the source format of the content into a platform format native to the content aggregation platform, receives the content location and the transformation instructions via the user interface, and stores the content location and the transformation instructions in a database to support subsequent delivery of the content to one or more users for display.