Abstract: Systems for obtaining an image of a target are provided including at least one multi-wavelength illumination module configured to illuminate a target using two or more different wavelengths, each penetrating the target at different depths; a multi-wavelength camera configured to detect the two or more different wavelengths illuminating the target on corresponding different channels and acquire corresponding images of the target based on the detected two or more different wavelengths illuminating the target; a control module configured synchronize illumination of the target by the at least one multi-wavelength illumination module and detection of the two or more different wavelengths by the camera; an analysis module configured to receive the acquired images of the target and analyze the acquired images to provide analysis results; and an image visualization module modify the acquired images based on the analysis results to provide a final improved image in real-time.

Abstract: Methods for calculating a MetaKG signal are provided. The method including illuminating a region of interest in a sample with a near-infrared (NIR) light source and/or a visible light source; acquiring images of the region of interest; processing the acquired images to obtain metadata associated with the acquired images; and calculating the MetaKG signal from the metadata associated with the acquired images. Related systems and computer program products are also provided.

Abstract: Systems for obtaining an image of a target are provided including at least one multi-wavelength illumination module configured to illuminate a target using two or more different wavelengths, each penetrating the target at different depths; a multi-wavelength camera configured to detect the two or more different wavelengths illuminating the target on corresponding different channels and acquire corresponding images of the target based on the detected two or more different wavelengths illuminating the target; a control module configured synchronize illumination of the target by the at least one multi-wavelength illumination module and detection of the two or more different wavelengths by the camera; an analysis module configured to receive the acquired images of the target and analyze the acquired images to provide analysis results; and an image visualization module modify the acquired images based on the analysis results to provide a final improved image in real-time.

Abstract: Methods for combining anatomical data and physiological data on a single image are provided. The methods include obtaining an image, for example, a raw near-infrared (NIR) image or a visible image, of a sample. The image of the sample includes anatomical structure of the sample. A physiologic map of blood flow and perfusion of the sample is obtained. The anatomical structure of the image and the physiologic map of the sample are combined into a single image of the sample. The single image of the sample displays anatomy and physiology of the sample in the single image in real time. Related systems and computer program products are also provided.

Abstract: Multispectral imaging systems are provided including a first light source having a first wavelength configured to image a sample; a second light source, different from the first light source, having a second wavelength, different from the first wavelength, configured to image the sample; and at least a third light source, different from the first and second light sources, having a third wavelength, different from the first and second wavelengths, configured to image the sample. A camera is configured to receive information related to the first, second and at least third light sources from the sample. A processor is configured to combine the information related to the first, second and at least third light sources provided by the camera to image an anatomical structure of the sample, image physiology of blood flow and perfusion of the sample and/or synthesize the anatomical structure and the physiology of blood flow and perfusion of the sample in terms of a blood flow rate distribution.

Abstract: Methods, systems and computer program products are provided for determining parameters during a surgical procedure. A plurality of patterns are projected onto an object plane associated with a target to be imaged. The plurality of patterns are manipulated such that the plurality of patterns overlap at a common point indicating a proper object distance from the target to be imaged.

Abstract: A fiber assembly is provided including a laser input end configured to receive an input signal having a first laser beam intensity. The fiber assembly further includes a plurality of channels attached to the laser input end and a plurality of laser safety adaptors. Each of the plurality of laser safety adaptors is configured to receive a corresponding one of the plurality of channels. A laser beam exiting each of the plurality of laser safety adaptors has a second laser beam intensity that is less than the first laser beam intensity.

Abstract: Some embodiments of the present inventive concept provide a system that uses two wavelengths of differential transmittance through a sample to apply laser speckle or laser Doppler imaging. A first of the two wavelengths is within the visible range that has zero or very shallow penetration. This wavelength captures the anatomical structure of tissue/organ surface and serves as a position marker of the sample but not the subsurface movement of blood flow and perfusion. A second wavelength is in the near Infra-Red (NIR) range, which has much deeper penetration. This wavelength reveals the underlying blood flow physiology and correlates both to the motion of the sample and also the movement of blood flow and perfusion. Thus, true motion of blood flow and perfusion can be derived from the NIR imaging measurement without being affected by the motion artifact of the target.

Abstract: An optical imaging system and related methods are provided that acquire images of an object at a distance in different spectral regions using only one camera. The systems and methods are adaptable to applications where information (simultaneous or sequential) from more than one spectral region is of interest while only one camera is available or entailed.

Abstract: Multispectral imaging systems are provided including a first light source having a first wavelength configured to image a sample; a second light source, different from the first light source, having a second wavelength, different from the first wavelength, configured to image the sample; and at least a third light source, different from the first and second light sources, having a third wavelength, different from the first and second wavelengths, configured to image the sample. A camera is configured to receive information related to the first, second and at least third light sources from the sample. A processor is configured to combine the information related to the first, second and at least third light sources provided by the camera to image an anatomical structure of the sample, image physiology of blood flow and perfusion of the sample and/or synthesize the anatomical structure and the physiology of blood flow and perfusion of the sample in terms of a blood flow rate distribution.

Abstract: Methods for calculating a MetaKG signal are provided. The method including illuminating a region of interest in a sample with a near-infrared (NIR) light source and/or a visible light source; acquiring images of the region of interest; processing the acquired images to obtain metadata associated with the acquired images; and calculating the MetaKG signal from the metadata associated with the acquired images. Related systems and computer program products are also provided.

Abstract: Methods for combining anatomical data and physiological data on a single image are provided. The methods include obtaining an image, for example, a raw near-infrared (NIR) image or a visible image, of a sample. The image of the sample includes anatomical structure of the sample. A physiologic map of blood flow and perfusion of the sample is obtained. The anatomical structure of the image and the physiologic map of the sample are combined into a single image of the sample. The single image of the sample displays anatomy and physiology of the sample in the single image in real time. Related systems and computer program products are also provided.

Abstract: Some embodiments of the present inventive concept provide a system that uses two wavelengths of differential transmittance through a sample to apply laser speckle or laser Doppler imaging. A first of the two wavelengths is within the visible range that has zero or very shallow penetration. This wavelength captures the anatomical structure of tissue/organ surface and serves as a position marker of the sample but not the subsurface movement of blood flow and perfusion. A second wavelength is in the near Infra-Red (NIR) range, which has much deeper penetration. This wavelength reveals the underlying blood flow physiology and correlates both to the motion of the sample and also the movement of blood flow and perfusion. Thus, true motion of blood flow and perfusion can be derived from the NIR imaging measurement without being affected by the motion artifact of the target.