Abstract: The present invention provides methods and system for easy detection of diseased tissue. The method of the present invention comprises the steps of quantifying fluorescence spectral values of a plurality of tissue regions of a target tissue, wherein the plurality of tissue regions represent stages of development of a disease; (b) transforming the fluorescence spectral values to produce enhanced spectral values for each tissue region; and (c) displaying an enhanced image of the plurality of tissue regions using the transformed spectral values, wherein the plurality of tissue regions appear substantially distinguishable.