Abstract: The present invention relates generally to the control of metabolic disorders, such as body weight disorders, of animals including mammals and humans, and more particularly to polypeptides identified herein as modulators of metabolic disorders, and to diagnostic and therapeutic uses of such modulators. In its broadest aspect, the present invention relates to mammalian neuronatin proteins sequences, isoforms thereof, and degenerate variations thereof, that demonstrate the ability to participate in the control of mammalian metabolism and that have been postulated to play a critical role in the regulation of body weight, adiposity and non-insulin dependent diabetes mellitus (NIDDM). The invention further relates to the preparation of the modulators of the invention.

Abstract: The invention relates to G-protein coupled receptor (GPCR) arrays, methods for production of GPCR arrays, primers used in the production of GPCR arrays and kits containing GPCR arrays and further to the use of such GPCR arrays in methods for the determination of expression profiles in biological materials in which there is an interest in the expression of GPCR polynucleotides.

Abstract: This invention relates to a method wherein special adaptors and primers are used for single stranded cDNA amplification. The method comprises contacting RNA with an anchored cDNA synthesis primer which can anneal to RNA, a suitable enzyme which possesses reverse transcriptase activity, and a special designed adaptor which can efficiently be ligated to the 3′ end of single stranded cDNA by T4 DNA ligase. Processes are provided for using specific primers annealing to the adaptors in the 5′ and 3′-end of the single stranded cDNA for PCR amplifying the cDNA. The invention describes the use of this approach for generation of probes starting from low amounts of RNA to be used e.g. in hybridization towards micro-arrays.