Patents by Inventor Kenneth Timmis
Kenneth Timmis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20140193868Abstract: The present invention is in the field of biosynthesis of polyhydroxyalkanoates (PHA). The invention relates to a genetically engineered microorganism having at least one gene involved in the metabolism, preferably in the production, of polyhydroxyalkanoates (PHA). This microorganism is useful in commercial production of polyhydroxyalkanoates. The present invention further relates to a method for the production of polyhydroxyalkanoates (PHA).Type: ApplicationFiled: January 7, 2014Publication date: July 10, 2014Inventors: Julia Sabirova, Peter Golyshin, Manuel Ferrer, Heinrich Lünsdorf, Wolf-Rainer Abraham, Kenneth Timmis
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Patent number: 8623632Abstract: The present invention is in the field of biosynthesis of polyhydroxyalkanoates (PHA). The invention relates to a genetically engineered microorganism having at least one gene involved in the metabolism, preferably in the production, of polyhydroxyalkanoates (PHA). This microorganism is useful in commercial production of polyhydroxyalkanoates. The present invention further relates to a method for the production of polyhydroxyalkanoates (PHA).Type: GrantFiled: August 9, 2006Date of Patent: January 7, 2014Assignee: Helmholtz-Zentrum fur Infektionsforschung GmbHInventors: Julia Sabirova, Peter Golyshin, Manuel Ferrer, Heinrich Lunsdorf, Wolf-Rainer Abraham, Kenneth Timmis
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Patent number: 8003357Abstract: The present invention relates to enzymes having catalytic activity at a pH below 5.0. The present invention provides hydrolyzing enzymes obtainable from archaeobacteria, in detail to hydrolytic enzymes obtainable from the archaeobacterium Ferroplasma acidiphilum. In general, the present invention provides enzymes which are active and stable at acidic pH values, especially at pH values from 1 to 4, especially in the range of pH 2 to 3, obtainable from Ferroplasma acidiphilum, especially to an esterase, glycosidases and a DNA ligase. In addition to stability and activity at low pH values, the enzymes according to the present invention are all dependent on Fe2+ for their catalytic activity.Type: GrantFiled: March 3, 2006Date of Patent: August 23, 2011Assignee: Helmholtz-Zentrum Fuer Infektionsforschung GmbHInventors: Olga Golyshina, Peter Golyshin, Kenneth Timmis, Manuel Ferrer
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Publication number: 20110183388Abstract: The present invention is in the field of biosynthesis of polyhydroxyalkanoates (PHA). The invention relates to a genetically engineered microorganism having at least one gene involved in the metabolism, preferably in the production, of polyhydroxyalkanoates (PHA). This microorganism is useful in commercial production of polyhydroxyalkanoates. The present invention further relates to a method for the production of polyhydroxyalkanoates (PHA).Type: ApplicationFiled: August 9, 2006Publication date: July 28, 2011Inventors: Julia Sabirova, Peter Golyshin, Manuel Ferrer, Heinrich Lunsdorf, Wolf-Rainer Abraham, Kenneth Timmis
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Patent number: 7811784Abstract: The invention relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, wherein at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts.Type: GrantFiled: October 11, 2004Date of Patent: October 12, 2010Assignee: Helmholtz-Zentrum für Infektionsforschung GmbHInventors: Manuel Ferrer, Kenneth Timmis, Tatjana Chernikova, Peter Golyshin, Michail Yakimov
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Publication number: 20100196965Abstract: The present invention relates to enzymes having catalytic activity at a pH below 5.0. The present invention provides hydrolyzing enzymes obtainable from archaeobacteria, in detail to hydrolytic enzymes obtainable from the archaeobacterium Ferroplasma acidiphilum. In general, the present invention provides enzymes which are active and stable at acidic pH values, especially at pH values from 1 to 4, especially in the range of pH 2 to 3, obtainable from Ferroplasma acidiphilum, especially to an esterase, glycosidases and a DNA ligase. In addition to stability and activity at low pH values, the enzymes according to the present invention are all dependent on Fe2+ for their catalytic activity.Type: ApplicationFiled: March 3, 2006Publication date: August 5, 2010Inventors: Olga Golyshina, Peter Golyshin, Kenneth Timmis, Manuel Ferrer
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Publication number: 20090305339Abstract: The invention relates to a new laccase from rumen, namely the RL5 laccase. In particular, the invention relates to a polypeptide comprising the amino acid sequence shown in FIG. 11 and comprising at least the amino acids No. 80 to No. 150 of the amino acid sequence shown in FIG. 11, respectively. The invention also relates to the use of polypeptides further comprising the amino acid sequence of a DUF152 domain with a core sequence as shown in FIG. 18 as laccases. Furthermore, the invention relates to methods for producing inventive polypeptides and their use.Type: ApplicationFiled: February 23, 2007Publication date: December 10, 2009Applicants: VIALACTIA BIOSCIENCES (NZ) LIMITED, HELMHOLTZ-ZENTRUM FUR INFEKTIONSFORSCHUNG GMBHInventors: Carsten Strompl, Manuel Ferrer, Tatyana Chernikova, Olga Golyshina, Kenneth Timmis, Kieran Elborough, Graeme Jarvis, Peter Golyshin
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Publication number: 20090255014Abstract: The invention relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, wherein at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts.Type: ApplicationFiled: October 11, 2004Publication date: October 8, 2009Inventors: Manuel Ferrer, Kenneth Timmis, Tatjana Chernikova, Peter Golyshin, Michail Yakimov
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Publication number: 20090078384Abstract: The invention provides polypeptides coding for new esterases from rumen. The invention also relates to functional fragments or functional derivatives thereof as well as to nucleic acids encoding the polypeptides of the invention, vectors and host cells containing said nucleic acids, a method for producing the polypeptides and the use of the polypeptides according to the present invention for various industrial purposes and medical treatments. The invention also relate to the conversion of said esterases into lipases.Type: ApplicationFiled: July 6, 2005Publication date: March 26, 2009Inventors: Manuel Ferrer, Peter Golyshin, Olga Golyshina, Tatyana Chernikova, Carsten Strompl, Kenneth Timmis, Kieran Elborough, Graeme Jarvis
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Publication number: 20080261267Abstract: The invention provides polypeptides coding for new cellulases from rumen, particularly from rumen ecosystem. The invention also relates to functional fragments or functional derivatives thereof as well as to nucleic acids encoding the polypeptides of the invention, vectors and host cells containing said nucleic acids, a method for producing the polypeptides and the use of the polypeptides according to the present invention for various industrial purposes and medical treatments.Type: ApplicationFiled: June 30, 2005Publication date: October 23, 2008Inventors: Manuel Ferrer, Peter Golyshin, Olga Golyshina, Tatyana Chernikova, Carsten Strompl, Kenneth Timmis, Kieran Elborough, Graeme Jarvis
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Publication number: 20080039407Abstract: The invention relates to a novel antibiotic from the macrolactin group and a microbial method for production of macrolactins and a novel isolated Bacillus subtilis, of application as the producing strain for the known Macrolactin A and the novel derivative.Type: ApplicationFiled: September 21, 2005Publication date: February 14, 2008Inventors: Kenneth Timmis, Gabriella Molinari, Rolf Jansen, Magally Romero-Tabarez, Dwi Santosa
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Patent number: 7115269Abstract: The present invention is directed to an attenuated Salmonella comprising a eukaryotic expression vector for delivery of the eukaryotic expression vector to a eukaryotic cell. Delivery may be to eukaryotic cells cultured in vitro or to cells in vivo, such as by oral administration of the attenuated Salmonella comprising the eukaryotic expression vector.Type: GrantFiled: October 18, 1999Date of Patent: October 3, 2006Assignee: Gesellschaft fuer Biotechnologische Forschung mbH (GBF)Inventors: Ayub Darji, Carlos A. Guzman, Kenneth Timmis, Siegfried Weiss, Birgit Gerstel, Trinad Chakraborty, Petra Wachholz, Jürgen Wehland
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Publication number: 20030180320Abstract: An attenuated strain of Salmonella typhimurium has been used as a vehicle for oral genetic immunization. Eukaryotic expression vectors containing the genes for b-galactosidase, or truncated forms of ActA and listeriolysin—two virulence factors of Listeria monocytogenes—that were controlled by an eukaryotic promoter have been used to transform a S. typhimurium aroA strain. Multiple or even single immunizations with these transformants induced a strong cytotoxic and helper T cell response as well as an excellent antibody response. Multiple immunizations with listeriolysin transformants protected the mice completely against a lethal challenge of L. monocytogenes. Partial protection was already observed with a single dose. ActA appeared not to be a protective antigen.Type: ApplicationFiled: October 18, 1999Publication date: September 25, 2003Inventors: AYUB DARJI, CARLOS A. GUZMAN, KENNETH TIMMIS, SIEGFRIED WEISS, BIRGIT GERSTEL, TRINAD CHAKRABORTY, PETRA WACHHOLZ
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Patent number: 5705361Abstract: The invention relates to a hybrid transposon having a PT operon, to plasmids and to bacterial strains (hosts) therefor.Type: GrantFiled: October 21, 1993Date of Patent: January 6, 1998Assignee: Gesellschaft fur Biotechnologische Forschung mbH GBFInventors: Mark Walker, Kenneth Timmis