Abstract: Provided herein are systems, kits, compositions and methods for sequencing library preparation and sequencing workflow (e.g., for the identification of mutations). In certain embodiments, provides herein systems and methods to identically barcode both strands of templates, and PCR-based enrichment of each strand that does not require hybridization capture.

Abstract: A heating device includes a combustion chamber, a housing in fluid communication with the combustion chamber, a burner disposed in the housing, a blower assembly connected to the housing for directing air into the interior of the housing, a valve assembly connected to the housing for controlling a flow of fuel into the burner, an optical color sensor for sensing a color profile of a surface of the burner, and a control unit configured to control the valve assembly in dependence upon the color profile of the surface of the burner.

Abstract: An increased efficiency boiler system includes a boiler, a thermostat in operative communication with the boiler and a controller. The controller is in communication with the boiler and the thermostat and selectively exchanges data with the boiler and the thermostat. The controller determines a proportional time period, reflecting how long the thermostat is requesting heat from the boiler within a predetermined time period, in dependence upon the exchange of data. The controller further determining a cycle frequency reflecting the number of times the thermostat is requesting heat from the boiler within the predetermined time period. The controller adjusts an energy input provided to the boiler in dependence upon the proportional time period and the cycle frequency.

Abstract: A heating device includes a combustion chamber, a housing in fluid communication with the combustion chamber, a burner disposed in the housing, a blower assembly connected to the housing for directing air into the interior of the housing, a valve assembly connected to the housing for controlling a flow of fuel into the burner, an optical color sensor for sensing a color profile of a surface of the burner, and a control unit configured to control the valve assembly in dependence upon the color profile of the surface of the burner.

Abstract: A modulating boiler system for heating a structure including a controller, a boiler operatively connected to the controller, and a thermostat operatively connected to the controller and boiler. The controller assesses a level of thermostat activity over a predetermined measuring period and adjusts the boiler in response to the level of activity to increase the boiler's efficiency.

Abstract: A hydronic air heater includes a frame assembly defining an enclosure, an inlet air damper formed in a wall of the enclosure and providing a means of ingress for ambient air, a closed heat transfer loop disposed within the enclosure, and a blower assembly disposed within the enclosure. The heat transfer loop includes a boiler for heating a fluid, a pump for circulating the fluid within the loop and a heating coil for receiving the heated fluid from the boiler. The blower assembly is configured to draw air into the enclosure through the inlet air damper and through the heating coil whereby heat from the fluid within the heating coil is transferred to the air.

Abstract: A modulating boiler system for heating a structure including a controller, a boiler operatively connected to the controller, and a thermostat operatively connected to the controller and boiler. The controller assesses a level of thermostat activity over a predetermined measuring period and adjusts the boiler in response to the level of activity to increase the boiler's efficiency.

Abstract: A method of increasing the efficiency of a boiler includes determining a proportional time period reflecting how long a thermostat is requesting heat from the boiler within a predetermined time period, determining a cycle frequency reflecting the number of times the thermostat is requesting heat from the boiler within the predetermined time period, and adjusting an energy input provided to the boiler in dependence upon the proportional time period and the cycle frequency.

Abstract: A hydronic air heater includes a frame assembly defining an enclosure, an inlet air damper formed in a wall of the enclosure and providing a means of ingress for ambient air, a closed heat transfer loop disposed within the enclosure, and a blower assembly disposed within the enclosure. The heat transfer loop includes a boiler for heating a fluid, a pump for circulating the fluid within the loop and a heating coil for receiving the heated fluid from the boiler. The blower assembly is configured to draw air into the enclosure through the inlet air damper and through the heating coil whereby heat from the fluid within the heating coil is transferred to the air.

Abstract: A fuel equalization system includes a filter box for accepting an air stream and a fuel stream, the air stream and the fuel stream mixing to form a mixed air/fuel stream. A blower is provided that has an inlet for accepting the mixed air/fuel stream from the filter box. An air deflection member is positioned in the path of the mixed air/fuel stream, between the filter box and the inlet, so as to reduce the turbulence of the mixed air/fuel stream.

Abstract: A modulating boiler system for heating a structure including a controller, a boiler operatively connected to the controller, and a thermostat operatively connected to the controller and boiler. The controller assesses a level of thermostat activity over a predetermined measuring period and adjusts the boiler in response to the level of activity to increase the boiler's efficiency.

Abstract: A modulating boiler system for heating a structure including a controller, a boiler operatively connected to the controller, and a thermostat operatively connected to the controller and boiler. The controller assesses a level of thermostat activity over a predetermined measuring period and adjusts the boiler in response to the level of activity to increase the boiler's efficiency.

Abstract: A modular boiler system includes a boiler control, a first boiler in operative connection with the boiler control, and a temperature sensor in operative connection with the first boiler. The system also features at least one secondary boiler in operative connection with the master boiler. The boiler control is operatively connected to only the first boiler and it enables the first boiler to control a boiler parameter of the first boiler and the at least one secondary boiler.

Abstract: A fuel equalization system includes a filter box for accepting an air stream and a fuel stream, the air stream and the fuel stream mixing to form a mixed air/fuel stream. A blower is provided that has an inlet for accepting the mixed air/fuel stream from the filter box. An air deflection member is positioned in the path of the mixed air/fuel stream, between the filter box and the inlet, so as to reduce the turbulence of the mixed air/fuel stream.

Abstract: A pilot and burner assembly includes a burner assembly and a blower for supplying combustion air to the burner assembly. The system further includes a pilot assembly and a fuel supply for providing fuel to the pilot assembly. A pilot fan provides pilot air to the pilot assembly, the pilot fan being separate from the blower and provides air only to the pilot assembly.

Abstract: A modulating boiler system for heating a structure including a controller, a boiler operatively connected to the controller, and a thermostat operatively connected to the controller and boiler. The controller assesses a level of thermostat activity over a predetermined measuring period and adjusts the boiler in response to the level of activity to increase the boiler's efficiency.

Abstract: A modular boiler system includes a boiler control, a first boiler in operative connection with the boiler control, and a temperature sensor in operative connection with the first boiler. The system also features at least one secondary boiler in operative connection with the master boiler. The boiler control is operatively connected to only the first boiler and it enables the first boiler to control a boiler parameter of the first boiler and the at least one secondary boiler.

Abstract: A boiler apparatus includes a housing defining an interior boiler chamber and a burner element arranged to be in thermal communication with the boiler chamber. An ignition device is provided for instigating combustion of an inlet gas stream, and is arranged adjacent one edge of the burner element. A gas restricting device is utilized for restricting contact between the burner element and the inlet gas stream such that the inlet gas stream is initially incident upon the one edge of the burner element, thereby forcing the inlet gas stream to propagate across the burner element from the one edge.

Abstract: A novel semi-instantaneous, modulating and condensing gas fired water heating system having a very high dynamic response for supplying potable water on demand at a substantially constant, controlled temperature having a maximum output of approximately 1,000,000 BTU/hour is provided. The device includes combustion means, heat exchanger means, and temperature control means having thermal measuring means and controlling means for controlling the rate of heat transfer in anticipation of changes in BTU requirements. The heat exchanger includes a combustion chamber open at its bottom to receive the combustible mixture of air and gas burned by the combustor. Enclosing the combustion chamber is a water chamber having a channel defined by the walls of the combustion and water chambers. Through the channel flows upwards the liquid, preferably water, to be heated.

Abstract: A self-contained, pilot-operated valve is provided with a plug for controlling the flow of a fluid through an orifice between inlet and outlet openings. A spring located about a stem projecting from the plug urges the plug into sealing engagement with the orifice. By means of a bellows surrounding the spring and having one end connected to the stem, an interior space is created, which space is in communication with the inlet opening through a restricted conduit. The interior space is also in communication with the outlet opening via a pilot valve. Operation of the pilot valve controls the pressure in the interior bellows space and hence the degree to which the bellows is compressed. This compression affects the plug stem and hence the position of the plug with respect to the valve seat.