Patents by Inventor Kenro Tokuhiro
Kenro Tokuhiro has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 10533169Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.Type: GrantFiled: June 27, 2018Date of Patent: January 14, 2020Assignees: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO, TOYOTA JIDOSHA KABUSHIKI KAISHAInventors: Satoshi Katahira, Risa Nagura, Kenro Tokuhiro, Nobuhiro Ishida, Chie Imamura, Toru Onishi, Noriko Yasutani, Nobuki Tada
-
Publication number: 20180298369Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.Type: ApplicationFiled: June 27, 2018Publication date: October 18, 2018Applicants: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO, TOYOTA JIDOSHA KABUSHIKI KAISHAInventors: Satoshi KATAHIRA, Risa NAGURA, Kenro TOKUHIRO, Nobuhiro ISHIDA, Chie IMAMURA, Toru ONISHI, Noriko YASUTANI, Nobuki TADA
-
Patent number: 10036005Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.Type: GrantFiled: March 28, 2014Date of Patent: July 31, 2018Assignees: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO, TOYOTA JIDOSHA KABUSHIKI KAISHAInventors: Satoshi Katahira, Risa Nagura, Kenro Tokuhiro, Nobuhiro Ishida, Chie Imamura, Toru Onishi, Noriko Yasutani, Nobuki Tada
-
Patent number: 9732338Abstract: The promoter of the present invention causes a desired gene to be highly expressed, especially in thermotolerant yeast. The promoter is located upstream of the PIR1 gene or the CTR1 gene on the Kluyveromyces marxianus chromosome and comprises a region controlling expression of the PIR1 gene or the CTR1 gene.Type: GrantFiled: March 4, 2013Date of Patent: August 15, 2017Assignees: TOYOTA JIDOSHA KABUSHIKI KAISHA, YAMAGUCHI UNIVERSITY, JX NIPPON OIL & ENERGY CORPORATIONInventors: Noriko Shisa, Rinji Akada, Hisashi Hoshida, Kozue Mutaguchi, Takeshi Uemura, Kenro Tokuhiro, Satoshi Katahira
-
Patent number: 9273328Abstract: A yeast of the genus Kluyveromyces is modified to improve the ethanol yield from xylose by attenuation of at least one gene selected from the group consisting of the ADH1 gene derived from Kluyveromyces marxianus, a gene functionally equivalent to the ADH1 gene, the ADH4 gene derived from Kluyveromyces marxianus, and a gene functionally equivalent to the ADH4 gene.Type: GrantFiled: March 26, 2012Date of Patent: March 1, 2016Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHAInventors: Noriko Shisa, Rinji Akada, Hisashi Hoshida, Takeshi Uemura, Kozue Mutaguchi, Kenro Tokuhiro, Satoshi Katahira
-
Publication number: 20160040151Abstract: A eukaryotic cell having xylose utilization ability. Provided is a protein that has xylose isomerase activity and has an amino acid sequence including, when aligned with an amino acid sequence expressed by SEQ ID NO:1, the 1st to 6th motifs expressed respectively by SEQ ID NOs:2 to 7 from the N-terminus side in the order described, and having, in place of asparagine (N) in an amino acid sequence of the 6th motif, another amino acid.Type: ApplicationFiled: March 28, 2014Publication date: February 11, 2016Inventors: Satoshi KATAHIRA, Risa NAGURA, Kenro TOKUHIRO, Nobuhiro ISHIDA, Chie IMAMURA, Toru ONISHI, Noriko YASUTANI, Nobuki TADA
-
Publication number: 20150031103Abstract: The promoter of the present invention causes a desired gene to be highly expressed, especially in thermotolerant yeast. The promoter is located upstream of the PIR1 gene or the CTR1 gene on the Kluyveromyces marxianus chromosome and comprises a region controlling expression of the PIR1 gene or the CTR1 gene.Type: ApplicationFiled: March 4, 2013Publication date: January 29, 2015Applicants: TOYOTA JIDOSHA KABUSHIKI KAISHA, JX NIPPON OIL & ENERGY CORPORATION, YAMAGUCHI UNIVERSITYInventors: Noriko Shisa, Rinji Akada, Hisashi Hoshida, Kozue Mutaguchi, Takeshi Uemura, Kenro Tokuhiro, Satoshi Katahira
-
Patent number: 8772012Abstract: It is an object of the disclosure of the present description to provide an eukaryotic cell having xylose utilization ability. The disclosure of the present description provides a novel eukaryotic cell having xylose utilization ability by transforming a yeast or other eukaryotic cell using DNA that codes a xylose isomerase from a termite protist.Type: GrantFiled: December 22, 2010Date of Patent: July 8, 2014Assignees: Kabushiki Kaisha Toyota Chuo Kenkyusho, RikenInventors: Satoshi Katahira, Kenro Tokuhiro, Nobuhiko Muramoto, Shigeharu Moriya, Moriya Ohkuma, Yoshina Takahashi
-
Publication number: 20140024097Abstract: A yeast of the genus Kluyveromyces is modified to improve the ethanol yield from xylose by attenuation of at least one gene selected from the group consisting of the ADH1 gene derived from Kluyveromyces marxianus, a gene functionally equivalent to the ADH1 gene, the ADH4 gene derived from Kluyveromyces marxianus, and a gene functionally equivalent to the ADH4 gene.Type: ApplicationFiled: March 26, 2012Publication date: January 23, 2014Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHAInventors: Noriko Shisa, Rinji Akada, Hisashi Hoshida, Takeshi Uemura, Kozue Mutaguchi, Kenro Tokuhiro, Satoshi Katahira
-
Publication number: 20130095538Abstract: It is an object of the disclosure of the present description to provide an eukaryotic cell having xylose utilization ability. The disclosure of the present description provides a novel eukaryotic cell having xylose utilization ability by transforming a yeast or other eukaryotic cell using DNA that codes a xylose isomerase from a termite protist.Type: ApplicationFiled: December 22, 2010Publication date: April 18, 2013Applicants: RIKEN, KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHOInventors: Satoshi Katahira, Kenro Tokuhiro, Nobuhiko Muramoto, Shigeharu Moriya, Moriya Ohkuma, Yoshina Takahashi
-
Publication number: 20120149081Abstract: The xylose-metabolizing ability and particularly the xylose incorporation rate, of yeast to which xylose-metabolizing ability has been imparted are significantly improved. The method according to the present invention comprises the steps of: culturing yeast having xylose-metabolizing ability in a xylose-containing medium in which the concentration of at least one amino acid selected from the group consisting of asparagine (Asn), serine (Ser), tyrosine (Tyr), threonine (Thr), and histidine (His) is increased; and recovering alcohol from the medium.Type: ApplicationFiled: December 8, 2011Publication date: June 14, 2012Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHAInventors: Noriko SHISA, Kenro TOKUHIRO, Satoshi KATAHIRA, Risa NAGURA
-
Patent number: 8039238Abstract: The present invention provides a transformant into which has been incorporated DNA for coding a foreign protein having lactate dehydrogenase activity and provided with pyruvic acid substrate affinity that equals or exceeds the pyruvic acid substrate affinity of the pyruvate decarboxylase inherent in the host organism. Said transformant can stably mass-produce lactic acid inside a host organism having the pyruvate decarboxylase gene.Type: GrantFiled: March 11, 2003Date of Patent: October 18, 2011Assignee: Toyota Jidosha Kabushiki KaishaInventors: Satoshi Saito, Osamu Saotome, Noriko Yasutani, Yasuo Matsuo, Nobuhiro Ishida, Masana Hirai, Takao Imaeda, Chikara Miyazaki, Kenro Tokuhiro
-
Patent number: 7964382Abstract: This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).Type: GrantFiled: November 26, 2008Date of Patent: June 21, 2011Assignees: Kabushiki Kaisha Toyota Chuo Kenkyusho, Toyota Jidosha Kabushiki KaishaInventors: Nobuhiro Ishida, Kenro Tokuhiro, Haruo Takahashi, Eiji Nagamori, Masana Hirai, Satoshi Saitoh, Tohru Ohnishi
-
Patent number: 7960511Abstract: The present teachings relate to an acid-resistant endoglucanase, which is a protein exhibiting excellent endoglucanase activity under acidic conditions. The present teachings provide a protein having the amino acid sequence set forth in SEQ ID NO: 2, a protein having an amino acid sequence with one or more amino acid modifications in the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity, or a protein having an amino acid sequence with at least 75% homology to the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity.Type: GrantFiled: April 9, 2009Date of Patent: June 14, 2011Assignee: Kabushiki Kaisha Toyota Chuo KenkyushoInventors: Nobuhiko Muramoto, Chie Imamura, Kenro Tokuhiro, Haruo Takahashi
-
Patent number: 7696333Abstract: The invention provides a promoter that can be used in the presence of an organic acid. The promoter includes DNA with promoter activity of high osmolarity response 7 gene (HOR7 gene), glycelaldehyde 3 phosphate dehydrogenase 2 gene (TDH2 gene), heat shock protein 30 gene (HSP30), hexose transport protein 7 gene (HXT7 gene), thioredoxin peroxidase 1 gene (AHP1 gene), or membrane protein 1 associated gene gene) of yeasts.Type: GrantFiled: November 5, 2004Date of Patent: April 13, 2010Assignees: Kabuhsiki Kaisha Toyota Chuo Jenjyusho, Toyota Jidosha Kabuhsiki KaishaInventors: Nobuhiro Ishida, Kenro Tokuhiro, Eiji Nagamori, Haruo Takahashi, Satoshi Saitoh, Tohru Ohnishi
-
Publication number: 20090298145Abstract: The present teachings relate to an acid-resistant endoglucanase, which is a protein exhibiting excellent endoglucanase activity under acidic conditions. The present teachings provide a protein having the amino acid sequence set forth in SEQ ID NO: 2, a protein having an amino acid sequence with one or more amino acid modifications in the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity, or a protein having an amino acid sequence with at least 75% homology to the amino acid sequence set forth in SEQ ID NO: 2 and having endoglucanase activity.Type: ApplicationFiled: April 9, 2009Publication date: December 3, 2009Applicant: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHOInventors: Nobuhiko Muramoto, Chie Imamura, Kenro Tokuhiro, Haruo Takahashi
-
Publication number: 20090275095Abstract: This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).Type: ApplicationFiled: November 26, 2008Publication date: November 5, 2009Inventors: Nobuhiro Ishida, Kenro Tokuhiro, Haruo Takahashi, Eiji Nagamori, Masana Hirai, Satoshi Saitoh, Tohru Ohnishi
-
Publication number: 20090104675Abstract: Lactic acid with high optical purity that has not previously been achieved is produced. It has been found that the optical purity of lactic acid is reduced as the racemization reaction of lactic acid proceeds when lactic acid coexists with glycerol. By reducing the amount of glycerol prior to concentrating lactic acid by heating, the optical purity of lactic acid after concentration by heating can be maintained at a high level.Type: ApplicationFiled: November 20, 2008Publication date: April 23, 2009Inventors: Ikuo Yamaguchi, Osamu Saotome, Shigeru Kuromiya, Tohru Ohnishi, Noriko Yasutani, Satoshi Saitoh, Makoto Mouri, Mitsuru Nakano, Arimitsu Usuki, Nobuhiro Ishida, Kenro Tokuhiro, Eiji Nagamori, Haruo Takahashi
-
Publication number: 20070161098Abstract: Lactic acid with high optical purity that has not previously been achieved is produced. It has been found that the optical purity of lactic acid is reduced as the racemization reaction of lactic acid proceeds when lactic acid coexists with glycerol. By reducing the amount of glycerol prior to concentrating lactic acid by heating, the optical purity of lactic acid after concentration by heating can be maintained at a high level.Type: ApplicationFiled: September 7, 2005Publication date: July 12, 2007Inventors: Ikuo Yamaguchi, Osamu Saotome, Shigeru Kuromiya, Tohru Ohnishi, Noriko Yasutani, Satoshi Saitoh, Makoto Mouri, Mitsuru Nakano, Arimitsu Usuki, Nobuhiro Ishida, Kenro Tokuhiro, Eiji Nagamori, Haruo Takahashi
-
Publication number: 20070105202Abstract: This invention provides a polynucleotide that encodes a protein having lactate dehydrogenase activity and such protein that can be used for producing D-lactic acid. This polynucleotide has the nucleotide sequence as shown in SEQ ID NO: 1 (a), and it hybridizes under stringent conditions with a probe comprising all or part of the nucleotide sequence as shown in SEQ ID NO: 1 or a complementary strand thereof and encodes a protein having D-lactate dehydrogenase activity (b).Type: ApplicationFiled: May 21, 2004Publication date: May 10, 2007Inventors: Nobuhiro Ishida, Kenro Tokuhiro, Haruo Takahashi, Eiji Nagamori, Masana Hirai, Satoshi Saitoh, Tohru Ohnishi