Abstract: The present invention relates to means and methods for improving protease expression by co-expression with a foldase.

Abstract: The present invention relates to means and methods for optimizing expression of a heterologous polypeptide of interest in Gram-positive host cells by co-expression with a foldase that is cognate to the heterologous polypeptide of interest.

Abstract: A progeny cell derived from a parent cell, wherein the progeny cell comprises at least one gene encoding MrgA protein or a functional homologue thereof and/or a DNA segment operably linked with the encoding gene, wherein said gene and/or DNA segment is manipulated with respect to the parent cell; the progeny cell comprises two or more copies of a gene encoding MrgA protein or a functional homologue thereof; or the progeny cell is mutated with respect to the parent cell; whereby the progeny cell produces greater amounts of MrgA protein or a functional homologue thereof than the parent cell.

Abstract: The present invention relates to a porphyrinogen based sensor having selective binding affinity for an explosive chemical. Upon binding of the target molecule, the porphyrinogen derivative undergoes a detectable adsorption and emission of electromagnetic radiation.

Abstract: A progeny cell derived from a parent cell, wherein the progeny cell comprises at least one gene encoding MrgA protein or a functional homologue thereof and/or a DNA segment operably linked with the encoding gene, wherein said gene and/or DNA segment is manipulated with respect to the parent cell; the progeny cell comprises two or more copies of a gene encoding MrgA protein or a functional homologue thereof; or the progeny cell is mutated with respect to the parent cell; whereby the progeny cell produces greater amounts of MrgA protein or a functional homologue thereof than the parent cell.

Abstract: A mutated bacterial cell producing at least one heterologous polypeptide of interest, wherein said cell has a reduced expression-level of a polypeptide comprising an amino acid sequence at least 70% identical to the sequence shown in SEQ ID NO: 2, when compared with an otherwise isogenic but non-mutated cell; methods for producing said mutated cell; and methods for producing a polypeptide of interest using said mutated cell. SEQ ID NO: 2 represents a putative metalloprotease.

Abstract: A mutated prokaryotic cell, which secretes higher amounts of at least one heterologous polypeptide of interest and which has a reduced expression-level of YusZ or YusX, or homologues thereof, when compared with an otherwise isogenic but non-mutated cell, and methods for constructing and using such a cell in the production of polypeptides.

Abstract: The present invention relates to a porphyrinogen based sensor having selective binding affinity for an explosive chemical. Upon binding of the target molecule, the porphyrinogen derivative undergoes a detectable adsorption and emission of electromagnetic radiation.

Abstract: A mutated bacterial cell producing at least one heterologous polypeptide of interest, wherein said cell has a reduced expression-level of YugJ (SEQ ID NO: 2) or a homologue thereof when compared with an otherwise isogenic but non-mutated cell; methods for producing said cell, and methods for producing a polypeptide of interest using said cell.

Abstract: The present invention relates to a method of identifying control sequences responding to antimicrobial peptides (AMPs), to host cells expressing an AMP and comprising a control sequence of the invention operable linked to a reporter, and to a method of screening for novel AMPs or AMP-variants having improved antimicrobial activity. Particularly the present invention relates to DNA control sequences, wherein the transcriptional profiles of said control sequences are regulated by the presence of an antimicrobial polypeptide expressed inside the host cell.

Abstract: A mutated prokaryotic cell, which secretes higher amounts of at least one heterologous polypeptide of interest and which has a reduced expression-level of YusZ or YusX, or homologues thereof, when compared with an otherwise isogenic but non-mutated cell, and methods for constructing and using such a cell in the production of polypeptides.

Abstract: A process for preparing monoesters comprises the step of reacting at least one diol with at least one carboxylic acid in a biphasic solvent system, the carboxylic acid being sufficiently water soluble to allow esterification to occur, and the biphasic solvent system comprising water and at least one aprotic solvent in which the resulting monoester has greater solubility than in water.