Abstract: Expression vectors and expression cassettes containing a tissue specific 5? transcription regulatory element, optionally linked to a translation regulatory element, optionally linked to an intron transcription regulatory element, operably linked to a heterologous polynucleotide encoding a protein or RNA of interest are described. The 5? transcription regulatory elements control expression in root tissue cells, phloem tissue cells, or fruit and/or abscission zone tissue cells. These sequences are obtained from citrus plants. Methods of use of these expression vectors and expression cassettes are described, as well as genetically altered plants and parts thereof that contain these expression vectors and/or cassettes.

Abstract: The present invention is directed to a combination blade and blade holder for performing a plurality of laboratory applications. The combination blade and blade holder comprises a blade having an elongated body portion with a front surface, a rear surface, a first edge and a second edge joined by way of a pair of opposing side edges, a blade cutting edge extending along the first edge and a blade handle formed along the second edge and the blade holder comprising a holder body having a top edge, a bottom edge, a front surface, a rear surface, a proximal end and a distal end, and a retaining flap positioned at the proximal end to receive and hold the blade firmly. The blade, the blade handle, the blade holder and the retaining flap are designed in such a way to hold the blade in position for performing the plurality of laboratory applications.

Abstract: The present invention is directed to a combination blade and blade holder for performing a plurality of laboratory applications. The combination blade and blade holder comprises a blade having an elongated body portion with a front surface, a rear surface, a first edge and a second edge joined by way of a pair of opposing side edges, a blade cutting edge extending along the first edge and a blade handle formed along the second edge and the blade holder comprising a holder body having a top edge, a bottom edge, a front surface, a rear surface, a proximal end and a distal end, and a retaining flap positioned at the proximal end to receive and hold the blade firmly. The blade, the blade handle, the blade holder and the retaining flap are designed in such a way to hold the blade in position for performing the plurality of laboratory applications.

Abstract: The present invention relates to isolated Solanum Bulbocastanum Bul409 promoter sequences and uses thereof. An exemplary embodiment provides an isolated plant Bul409 promoter comprising a nucleic acid sequence that is at least about 90% identical to nucleotides 1-771 of SEQ ID NO:1, wherein the promoter sequence is capable of controlling transcription in a plant. Other exemplary embodiments provide a method for making a transgenic plant, wherein the method comprises transforming a plant, plant part, or plant cell with an expression vector comprising isolated plant Bul409 promoter operably linked to a heterologous nucleic acid sequence, wherein the isolated plant Bul409 promoter is capable of controlling transcription of the heterologous nucleic acid in a plant, and a transgenic plant made by the method and decendants thereof.

Abstract: The present invention relates to isolated Solanum Bulbocastanum Bul427 promoter sequences and uses thereof. An exemplary embodiment provides an isolated plant Bul427 promoter comprising a nucleic acid sequence that is at least about 90% identical to nucleotides 1-1154 of SEQ ID NO:1, wherein the promoter sequence is capable of controlling transcription in a plant. Other exemplary embodiments provide a method for making a transgenic plant, wherein the method comprises transforming a plant, plant part, or plant cell with an expression vector comprising isolated plant Bul427 promoter operably linked to a heterologous nucleic acid sequence, wherein the isolated plant Bul427 promoter is capable of controlling transcription of the heterologous nucleic acid in a plant, and a transgenic plant made by the method and decendants thereof.

Abstract: The present invention is directed to a combination blade and blade holder for performing a plurality of laboratory applications. The combination blade and blade holder comprises a blade having an elongated body portion with a front surface, a rear surface, a first edge and a second edge joined by way of a pair of opposing side edges, a blade cutting edge extending along the first edge and a blade handle formed along the second edge and the blade holder comprising a holder body having a top edge, a bottom edge, a front surface, a rear surface, a proximal end and a distal end, and a retaining flap positioned at the proximal end to receive and hold the blade firmly. The blade, the blade handle, the blade holder and the retaining flap are designed in such a way to hold the blade in position for performing the plurality of laboratory applications.

Abstract: The present invention relates to isolated Solanum Bulbocastanum Bul409 promoter sequences and uses thereof.

Abstract: The present invention relates to isolated Solanum Bulbocastanum Bul427 promoter sequences and uses thereof

Abstract: Nucleic acid sequences from potato that encode the enzyme ?-solanine/?-chaconine rhamnosyltransferase (SGT3) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT3 and thereby reduce levels of the predominant steroidal glycoalkaloids ?-chaconine and ?-solanine in Solanaceous plants such as potato are described.

Abstract: Nucleic acid sequences from potato that encode the enzyme UDP-glucose:solanidine glucosyltransferase (SGT2) are disclosed. Recombinant DNA molecules containing the sequences, and use thereof, in particular, use of the sequences and antisense constructs to inhibit the production of SGT2 and thereby reduce the level of the more human-toxic of the two predominant steroidal glycoalkaloids ?-chaconine and/or increase the level of the more insect-toxic ?-solanine in Solanaceous plants such as potato are described.

Abstract: A Solanum bulbocastanum gene has been cloned from a late blight-resistance locus and its sequence provided, together with the sequence of the encoded protein. DNA encoding the resistance protein has been introduced into potato plants and confers resistance to Phytophthora infestans, the causal agent of late blight. The resistance protein is in the class of Nucleotide Binding Site-Leucine-Rich Repeat Proteins (NBS-LRRP), and the gene in S. bulbocastanum is flanked by related NBS-LRRP gene sequences.