Patents by Inventor Kenta FUKUNAGA

Kenta FUKUNAGA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for producing sulfated polysaccharide and method for producing PAPS
    Patent number: 12264350
    Abstract: The invention provides a method for producing sulfated polysaccharides by reacting a PAPS production/regeneration system utilizing the metabolic activity of a microorganism or a treated matter thereof with a microorganism expressing a sulfation enzyme or a treated matter or extract thereof upon mixing of inexpensive raw materials such as magnesium sulfate. The invention also provides a method for producing PAPS from inexpensive raw materials. The methods involve preparing a transformant (a) of a bacterium of the genus Corynebacterium, which contains a gene encoding an ATP sulfurylase and a gene encoding an APS kinase, which are expressible, and in which a cell plasma membrane of the transformant (a) is substance-permeable, or a treated matter of the transformant (a), and conducting a reaction for producing PAPS by using a reaction solution containing ATP or an ATP source, a sulfate ion source, and the transformant (a) or the treated matter thereof.
    Type: Grant
    Filed: April 2, 2021
    Date of Patent: April 1, 2025
    Assignees: RENSSELAER POLYTECHNIC INSTITUTE, OTSUKA PHARMACEUTICAL FACTORY, INC., KIRIN BIOMATERIALS CO., LTD.
    Inventors: Kenta Fukunaga, Aki Nishibara, Ryosuke Kato, Masahiro Kuratsu, Mikiro Hayashi, Shin-ichi Hashimoto
  • METHOD FOR PRODUCING SULFATED POLYSACCHARIDE AND METHOD FOR PRODUCING PAPS
    Publication number: 20230159969
    Abstract: The invention provides a method for producing sulfated polysaccharides by reacting a PAPS production/regeneration system utilizing the metabolic activity of a microorganism or a treated matter thereof with a microorganism expressing a sulfation enzyme or a treated matter or extract thereof upon mixing of inexpensive raw materials such as magnesium sulfate. The invention also provides a method for producing PAPS from inexpensive raw materials. The methods involve preparing a transformant (a) of a bacterium of the genus Corynebacterium, which contains a gene encoding an ATP sulfurylase and a gene encoding an APS kinase, which are expressible, and in which a cell plasma membrane of the transformant (a) is substance-permeable, or a treated matter of the transformant (a), and conducting a reaction for producing PAPS by using a reaction solution containing ATP or an ATP source, a sulfate ion source, and the transformant (a) or the treated matter thereof.
    Type: Application
    Filed: April 2, 2021
    Publication date: May 25, 2023
    Applicants: RENSSELAER POLYTECHNIC INSTITUTE, OTSUKA PHARMACEUTICAL FACTORY, INC., KIRIN BIOMATERIALS CO., LTD.
    Inventors: Kenta FUKUNAGA, Aki NISHIBARA, Ryosuke KATO, Masahiro KURATSU, Mikiro HAYASHI, Shin-ichi HASHIMOTO