Abstract: The present invention provides an organelle transformant screening method. More specifically, the present invention provides an organelle transformant screening method utilizing inhibition of a lipid synthetic pathway.

Abstract: A toner comprising a toner particle that contains a binder resin and a wax, wherein the toner particle contains an organosilicon polymer as given by formula (1) below; R1—SiO3/2??(1), in formula (1), R1 is an alkyl group having from 1 to 4 carbons; in a cross section of the toner particle observed using a transmission electron microscope, in terms of a distance A from the centroid of the cross section of the toner particle to a contour of the cross section, the organosilicon polymer is present in a region that is not more than 80% of the distance A from the centroid; and an absolute value of a difference between an SP value of the organosilicon polymer and an SP value of the wax is not more than 0.70 (cal/cm3)1/2.

Abstract: Provided is a toner containing a toner particle comprising a binder resin and an organosilicon polymer, wherein the organosilicon polymer contains an organosilicon polymer particle, the organosilicon polymer particle has a structure represented by the following formula (1): R1—SiO3/2??(1) in formula (1), R1 is a C1-4 alkyl group, and in cross-sectional observation of the toner particle by transmission electron microscopy, the organosilicon polymer particle is present in a region extending up to 80% of the distance A from the centroid out of the total distance A from the centroid of the toner particle cross-section to the contour of the cross-section, and the particle diameter of the organosilicon polymer particle is from 10 nm to 200 nm.

Abstract: The present invention provides a method for selecting a promoter that functions in an organelle, the method comprising: (1) the step of preparing sequence information obtained by RNA sequencing analysis; (2) the step of mapping the sequence information prepared in the step (1) onto a sequence of organellar DNA; (3) the step of calculating the amount of change in RNA expression in each region based on the mapping information obtained in the step (2); (4) the step of selecting regions in which the amount of change obtained in the step (3) is within a range of preset reference values; and (5) the step of identifying a region, among the regions selected, as a promoter functioning in an organelle.

Abstract: A toner comprising a toner particle that contains a binder resin and a wax, wherein the toner particle contains an organosilicon polymer as given by formula (1) below; R1-SiO3/2 ??(1), in formula (1),R1 is an alkyl group having from 1 to 4 carbons; in a cross section of the toner particle observed using a transmission electron microscope, in terms of a distance A from the centroid of the cross section of the toner particle to a contour of the cross section, the organosilicon polymer is present in a region that is not more than 80% of the distance A from the centroid; and an absolute value of a difference between an SP value of the organosilicon polymer and an SP value of the wax is not more than 0.70 (cal/cm3)1/2.

Abstract: Provided is a toner containing a toner particle comprising a binder resin and an organosilicon polymer, wherein the organosilicon polymer contains an organosilicon polymer particle, the organosilicon polymer particle has a structure represented by the following formula (1): R1—SiO3/2 ??(1) in formula (1), R1 is a C1-4 alkyl group, and in cross-sectional observation of the toner particle by transmission electron microscopy, the organosilicon polymer particle is present in a region extending up to 80% of the distance A from the centroid out of the total distance A from the centroid of the toner particle cross-section to the contour of the cross-section, and the particle diameter of the organosilicon polymer particle is from 10 nm to 200 nm.

Abstract: A toner has a toner particle including a binder resin, a colorant, and a wax, in which in a cross section of the toner particle observed under a scanning transmission electron microscope, a proportion of the number of toner particles having a void with a long diameter of 0.50 ?m or less is 60 number % or more, and a proportion of the number of toner particles having a void in a region from an outline of the cross section of the toner particle inward to 1.00 ?m in a total number of the toner particles having a void is 30 number % or less.

Abstract: A method for inducing a structural change in a plant mitochondrial genome is provided. The invention relates to a method for inducing a structural change in a mitochondrial genome in a plant cell by introducing a double-strand break into a target sequence region on mitochondrial genomic DNA in a plant cell, which is present in individual molecule species of the mitochondrial genomic DNA. In addition, the present invention also relates to a method for deleting a gene that is present in mitochondrial genomic DNA in a plant cell by introducing a double-strand break into the gene or a region near the gene, which is present in individual molecule species of the mitochondrial genomic DNA. Moreover, the invention also relates to a plant cell having a mitochondrial genome, in which a structural change has been induced by the method, and a seed and a plant having the plant cell.

Abstract: A toner has a toner particle including a binder resin, a colorant, and a wax, in which in a cross section of the toner particle observed under a scanning transmission electron microscope, a proportion of the number of toner particles having a void with a long diameter of 0.50 ?m or less is 60 number % or more, and a proportion of the number of toner particles having a void in a region from an outline of the cross section of the toner particle inward to 1.00 ?m in a total number of the toner particles having a void is 30 number % or less.

Abstract: A method for inducing a structural change in a plant mitochondrial genome is provided. The invention relates to a method for inducing a structural change in a mitochondrial genome in a plant cell by introducing a double-strand break into a target sequence region on mitochondrial genomic DNA in a plant cell, which is present in individual molecule species of the mitochondrial genomic DNA. In addition, the present invention also relates to a method for deleting a gene that is present in mitochondrial genomic DNA in a plant cell by introducing a double-strand break into the gene or a region near the gene, which is present in individual molecule species of the mitochondrial genomic DNA. Moreover, the invention also relates to a plant cell having a mitochondrial genome, in which a structural change has been induced by the method, and a seed and a plant having the plant cell.