Patents by Inventor Kenta Sumiyama

Kenta Sumiyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10863730
    Abstract: A method for producing a cell in which a target gene is knocked out, the method including the step of: introducing a CRISPR-Cas system into a cell having one or more kinds of target genes, the CRISPR-Cas system being able to produce (i) three or more kinds of guide RNAs for each of the one or more kinds of target genes and (ii) a Cas protein. The present invention can provide a method that enables highly efficient (90% or more) production of whole-body biallelic knockout animals in a single generation.
    Type: Grant
    Filed: December 25, 2015
    Date of Patent: December 15, 2020
    Assignee: RIKEN
    Inventors: Hiroki Ueda, Genshiro Sunagawa, Kenta Sumiyama, Maki Ukai, Dimitri Perrin
  • Publication number: 20180020646
    Abstract: A method for producing a cell in which a target gene is knocked out, the method including the step of: introducing a CRISPR-Cas system into a cell having one or more kinds of target genes, the CRISPR-Cas system being able to produce (i) three or more kinds of guide RNAs for each of the one or more kinds of target genes and (ii) a Cas protein. The present invention can provide a method that enables highly efficient (90% or more) production of whole-body biallelic knockout animals in a single generation.
    Type: Application
    Filed: December 25, 2015
    Publication date: January 25, 2018
    Inventors: Hiroki UEDA, Genshiro SUNAGAWA, Kenta SUMIYAMA, Maki UKAI, Dimitri PERRIN
  • Patent number: 9103790
    Abstract: A linker for a unimolecular FRET biosensor based on a principle of fluorescence resonance energy transfer, the linker including: a polypeptide containing 52 to 400 amino acids residues, wherein at least 45% of a total number of the amino acid residues are glycine, alanine, or both thereof, and at least 10% of the total number of the amino acid residues are alanine.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: August 11, 2015
    Assignee: KYOTO UNIVERSITY
    Inventors: Michiyuki Matsuda, Naoki Komatsu, Kazuhiro Aoki, Yuji Kamioka, Hiroko Yukinaga, Yoshie Inaoka, Atsuro Sakurai, Etsuko Kiyokawa, Kenta Sumiyama
  • Patent number: 6917883
    Abstract: A method is described for efficiently searching non-coding DNA for known control elements. Short conserved DNA sequences can be identified among 3 or more species by selecting DNA sequences from species having a total genetic distance larger than one substitution per site at a neutrally evolving region and calculating the total substitutions using pair-wise genetic distances, with the pairs for comparison selected based on phylogenetic relationships.
    Type: Grant
    Filed: January 15, 2003
    Date of Patent: July 12, 2005
    Assignee: Yale University
    Inventors: Frank H. Ruddle, Kenta Sumiyama, Chang-Bae Kim