Abstract: The present invention relates to an improved technology for a device that identifies and diagnoses an environmental stress state of plants. An environmental stress diagnosis device comprises a measurement light source 12, an induction light source 14, and a transmitted light detector 18. The measurement light source 12 radiates a first measurement light ML1 and a second measurement light ML2 to a plant sample S, the induction light source 14 radiates a first photosynthesis inducing light FR and a second photosynthesis inducing light AL to the plant sample S, and the transmitted light detector 18 detects a first transmitted light TL1 and a second transmitted light TL2. The control unit 20 has an analysis circuit 20a and a control circuit 20b.

Abstract: A method of transferring an exogenous gene into a eukaryotic algal cell, the method including performing electroporation using multiple square-wave pulses to the solution containing a cell of a green alga with cell-wall, and a nucleic acid molecule by the following three steps: applying a square-wave electric pulse (first electric pulse) with a high voltage for a short period of time under the condition that its total electric energy falls within a predetermined range; then applying a square-wave electric pulse (second electric pulse) with a low voltage for a long period of time two or more times; and then applying a square-wave electric pulse (third electric pulse) that is opposite in polarity to the second electric pulse, with a low voltage for a long period of time, two or more times.

Abstract: To provide a technology that solves the above-mentioned problems, and is directly applicable to eukaryotic algal cells with cell-wall, the technology enabling gene transfer and transformation to be performed with high efficiency and good reproducibility irrespective of species of algae.