Patents by Inventor Kentaro Nagamine
Kentaro Nagamine has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8017357Abstract: The present invention provides a nucleic acid synthesis method which involves the step of incubating a double-stranded nucleic acid template under conditions that ensure a complementary strand synthesis reaction using a primer as an origin. This method involves the step of placing a region, to which a primer capable of isothermally amplifying the template nucleic acid will anneal, in a condition that ensures base pairing, using an arbitrary primer. The arbitrary primer initiates the complementary strand synthesis reaction, using the double-stranded nucleic acid as a template and DNA polymerases catalyzing the complementary strand synthesis reaction which comprises the destabilization of the double-stranded nucleic acid and strand displacement, thereby providing a region that can undergo base pairing.Type: GrantFiled: March 30, 2001Date of Patent: September 13, 2011Assignee: Eiken Kagaku Kabushiki KaishaInventors: Tsugunori Notomi, Kentaro Nagamine
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Patent number: 7851186Abstract: The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction.Type: GrantFiled: April 15, 2008Date of Patent: December 14, 2010Assignee: Eiken Kagaku Kabushiki KaishaInventor: Kentaro Nagamine
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Patent number: 7846695Abstract: The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction.Type: GrantFiled: April 15, 2008Date of Patent: December 7, 2010Assignee: Eiken Kagaku Kabushiki KaishaInventor: Kentaro Nagamine
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Patent number: 7745135Abstract: A method for detecting the occurrence of nucleic acid syntheses using an enzyme through the use of a generated insoluble substance as an indicator.Type: GrantFiled: April 21, 2008Date of Patent: June 29, 2010Assignee: Eiken Kagaku Kabushiki KaisyaInventors: Yasuyoshi Mori, Kentaro Nagamine
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Patent number: 7638280Abstract: The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3?-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.Type: GrantFiled: December 22, 2006Date of Patent: December 29, 2009Assignee: Eiken Kagaku Kabushiki KaishaInventors: Hidetoshi Kanda, Tsugunori Notomi, Kentaro Nagamine, Toshihiro Yonekawa
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Publication number: 20090081664Abstract: A method for detecting the occurrence of nucleic acid syntheses using an enzyme through the use of a generated insoluble substance as an indicator.Type: ApplicationFiled: April 21, 2008Publication date: March 26, 2009Applicant: Eiken Kagaku Kabushiki KaisyaInventors: Yasuyoshi Mori, Kentaro Nagamine
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Publication number: 20080213842Abstract: The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction.Type: ApplicationFiled: April 15, 2008Publication date: September 4, 2008Applicant: EIKEN KAGAKU KABUSHIKI KAISHAInventor: Kentaro Nagamine
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Publication number: 20080213790Abstract: The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction.Type: ApplicationFiled: April 15, 2008Publication date: September 4, 2008Applicant: EIKEN KAGAKU KABUSHIKI KAISHAInventor: Kentaro Nagamine
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Patent number: 7374879Abstract: A method for detecting the occurrence of nucleic acid syntheses using an enzyme through the use of a generated insoluble substance as an indicator.Type: GrantFiled: April 25, 2001Date of Patent: May 20, 2008Assignee: Eiken Kagaku Kabushiki KaisyaInventors: Yasuyoshi Mori, Kentaro Nagamine
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Patent number: 7374913Abstract: The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction.Type: GrantFiled: September 19, 2001Date of Patent: May 20, 2008Assignee: Eiken Kagaku Kabushiki KaishaInventor: Kentaro Nagamine
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Publication number: 20070238113Abstract: The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3?-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.Type: ApplicationFiled: December 22, 2006Publication date: October 11, 2007Applicant: EIKEN KAGAKU KABUSHIKI KAISHAInventors: Hidetoshi Kanda, Tsugunori Notomi, Kentaro Nagamine, Toshihiro Yonekawa
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Patent number: 7175985Abstract: The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3?-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.Type: GrantFiled: August 2, 2002Date of Patent: February 13, 2007Assignee: Eiken Kagaku Kabushiki KaishaInventors: Hidetoshi Kanda, Tsugunori Notomi, Kentaro Nagamine, Toshihiro Yonekawa
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Publication number: 20060141452Abstract: This invention relates to a method for selectively and efficiently synthesizing one of the sense or antisense strands of double-stranded nucleic acid. The method for synthesizing single-stranded nucleic acid according to this invention comprises the following steps of: 1) cleaving, with a restriction enzyme, double-stranded DNA having a restriction enzyme recognition sequence at a portion closer to the 5? side than the target sequence in such a manner that a) a fragment having an overhanging 3? terminus is formed, and b) base sequences of the single-stranded regions of the fragments after the cleavage differ from each other; 2) to the single-stranded region of the DNA fragment that was cleaved with the restriction enzyme, annealing a primer having a base sequence complementary to the region on at least its 3? terminus; and 3) synthesizing a nucleic acid by a strand displacement-type polymerase starting from the 3? terminus of the primer.Type: ApplicationFiled: October 26, 2001Publication date: June 29, 2006Inventor: Kentaro Nagamine
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Publication number: 20040038253Abstract: The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction.Type: ApplicationFiled: June 18, 2003Publication date: February 26, 2004Inventor: Kentaro Nagamine
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Publication number: 20030207292Abstract: The present invention provides a nucleic acid synthesis method which involves the step of incubating a double-stranded nucleic acid template under conditions that ensure a complementary strand synthesis reaction using a primer as an origin. This method involves the step of placing a region, to which a primer capable of isothermally amplifying the template nucleic acid will anneal, in a condition that ensures base pairing, using an arbitrary primer. The arbitrary primer initiates the complementary strand synthesis reaction, using the double-stranded nucleic acid as a template and DNA polymerases catalyzing the complementary strand synthesis reaction which comprises the destabilization of the double-stranded nucleic acid and strand displacement, thereby providing a region that can undergo base pairing.Type: ApplicationFiled: April 8, 2003Publication date: November 6, 2003Inventors: Tsugunori Notomi, Kentaro Nagamine
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Publication number: 20030129632Abstract: A method for detecting the occurrence of nucleic acid syntheses using an enzyme through the use of a generated insoluble substance as an indicator.Type: ApplicationFiled: November 1, 2002Publication date: July 10, 2003Inventors: Yasuyoshi Mori, Kentaro Nagamine
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Patent number: 6548272Abstract: A gene for a novel transmembrane protein was isolated and its nucleotide sequence was determined. A protein encoded by this gene (TRPC7) has 7 transmembrane regions, and because there is homology with TRP (transient receptor potential), this protein is considered to have calcium channel functions. Further, since a mutation in the TRPC7 gene is frequently observed in patients with manic-depressives insanity, TRPC7 is considered to be a pathogenic gene for bipolar affective disorder.Type: GrantFiled: July 11, 2000Date of Patent: April 15, 2003Assignee: Eiken Kagaku Kabushiki KaisyaInventors: Nobuyoshi Shimizu, Kentaro Nagamine