Patents by Inventor Kenton C. Hasson

Kenton C. Hasson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10376891
    Abstract: The present invention relates to systems and methods of temperature referencing for melt curve data collection. More specifically, the present invention relates to systems and methods for collecting DNA melt curve data for a DNA sample and a temperature reference material.
    Type: Grant
    Filed: July 18, 2016
    Date of Patent: August 13, 2019
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Kenton C. Hasson, Hiroshi Inoue
  • Patent number: 10363558
    Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.
    Type: Grant
    Filed: August 24, 2015
    Date of Patent: July 30, 2019
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Ivor T. Knight, Kenton C. Hasson, Johnathan S. Coursey, Hongye Liang, Sami Kanderian, Gregory H. Owen, Weidong Cao, Ying-Xin Wang, Scott Corey, Ben Lane, Conrad Laskowski, Alex Flamm, Brian Murphy, Eric Schneider, Takayoshi Hanagata, Hiroshi Inoue, Shulin Zeng, Brian Bean, Franklin Regan
  • Patent number: 10266873
    Abstract: This invention relates to systems and methods for imaging sample materials within a microfluidic device during an assay reaction process. In accordance with certain aspects of the invention, images are formed with a pixel array and a region of interest (“ROI”) is defined within the pixel array. Image values, such as fluorescent intensity, can be computed as averages of individual pixel values within the ROI. Where the ROI is subject to non-uniform conditions, such as non-uniform heating, the ROI can be divided into sub-ROIs which are sufficiently small that the condition is uniform within the sub-ROI.
    Type: Grant
    Filed: July 31, 2013
    Date of Patent: April 23, 2019
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Hongye Liang, Kenton C. Hasson
  • Patent number: 10226772
    Abstract: The invention relates to systems and methods including a combination of thermal generating device technologies to achieve more efficiency and accuracy in PCR temperature cycling of nucleic samples undergoing amplification.
    Type: Grant
    Filed: February 29, 2016
    Date of Patent: March 12, 2019
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Gregory A. Dale, Shulin Zeng, Kenton C. Hasson
  • Patent number: 10092902
    Abstract: An interface cartridge for a microfluidic chip, with microfluidic process channels and fluidic connection holes at opposed ends of the process channels, provides ancillary fluid structure, including fluid flow channels and input and/or waste wells, which mix and/or convey reaction fluids to the fluidic connection holes and into the process channels of the microfluidic chip.
    Type: Grant
    Filed: September 21, 2015
    Date of Patent: October 9, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Ray Tsao, Hiroshi Inoue, Shulin Zeng, Brian Murphy, Kenton C. Hasson
  • Patent number: 10066977
    Abstract: The present invention relates to systems and methods of monitoring velocity or flow in channels, especially in microfluidic channels. In some embodiments, the present invention relates to systems and methods of monitoring velocity or flow rate in systems and methods for performing a real-time polymerase chain reaction (PCR) in a continuous-flow microfluidic system.
    Type: Grant
    Filed: January 26, 2009
    Date of Patent: September 4, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Kenton C. Hasson, Gregory A. Dale
  • Publication number: 20180236444
    Abstract: A microfluidic chip having integrated heaters and a method for manufacturing the microfluidic chip is provided. Specifically, the microfluidic chip comprises a first substrate having a microchannel formed therein. The second substrate is bonded to the first substrate to encapsulate the microchannel. An integrated heating element, that is hermetically sealed and electrically isolated from the microchannel, is formed on the top surface the second substrate after the first and second substrates are bonded together. A biological reaction can be performed in the microchannel of the microfluidic chip while the fluid in the microchannel is heated by electrical current passing through the integrated heating element.
    Type: Application
    Filed: July 2, 2015
    Publication date: August 23, 2018
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Kenton C. Hasson, Andrea Pais, Brian Jamieson
  • Publication number: 20180193839
    Abstract: The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.
    Type: Application
    Filed: January 5, 2018
    Publication date: July 12, 2018
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Sami Kanderian, Gregory H. Owen, Hongye Liang, Scott Corey, Brian Bean
  • Publication number: 20180179602
    Abstract: Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.
    Type: Application
    Filed: February 26, 2018
    Publication date: June 28, 2018
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Brian Bean, Scott Corey
  • Patent number: 9939336
    Abstract: The invention relates to systems and methods for calibrating and using resistance temperature detectors. In one embodiment, the system includes a calibration circuit comprising a resistance temperature detector in a bridge circuit with at least one potentiometer, and a programmable gain amplifier coupled to the bridge circuit. Embodiments of the invention further comprise methods for calibrating the bridge circuit and the programmable gain amplifier for use with the resistance temperature detector and methods for determining the self heating voltage of the bridge circuit.
    Type: Grant
    Filed: August 4, 2014
    Date of Patent: April 10, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Gregory H. Owen
  • Patent number: 9919314
    Abstract: Systems and methods for air cooling a microfluidic device using confinement channels to isolate cooling air from exposed liquids are disclosed. The systems and methods may also thermally condition the cooling airflow for improved robustness of the microfluidic device. In one embodiment, the air cooling system includes a split-level cooling manifold including an inlet duct that directs cooling air to a microfluidic device and an outlet duct that directs air heated by the microfluidic device away from the microfluidic device. The temperature of cooling air may be measured. The cooling air may be preheated to a temperature that is higher than an expected ambient temperature. The temperature of the cooling air after being heated by a microfluidic device may be measured.
    Type: Grant
    Filed: October 26, 2015
    Date of Patent: March 20, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Ben Lane, Eric Schneider
  • Publication number: 20180056299
    Abstract: An apparatus for performing a thermocyclic process, such as amplifying DNA, includes a microfluidic chip with a channel formed therein and one or more thermal distribution elements disposed over portions of the chip. Each thermal distribution element is configured to distribute thermal energy from an external thermal energy source substantially uniformly over the portion of the chip covered by the thermal distribution element. The portion of the chip covered by the thermal distribution element thereby comprises a discrete temperature zone. Other temperature zones can be defined by other thermal distribution elements or by portions of the chip not covered by a thermal distribution element. The channel is configured so that a fluid flowing through the channel would enter and exit the different temperature zones a plurality of times, thereby alternately exposing the fluid to the temperature of each zone for a period of time required for the fluid to traverse the zone.
    Type: Application
    Filed: August 7, 2017
    Publication date: March 1, 2018
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Gregory A. Dale, Kenton C. Hasson, Shulin Zeng, Michele Stone
  • Patent number: 9903003
    Abstract: Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 27, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Brian Bean, Scott Corey
  • Publication number: 20180030513
    Abstract: A system and method for amplifying and detecting nucleic acids are disclosed. In one embodiment, the system includes: a microfluidic device comprising a channel for receiving a sample of solution containing real-time PCR reagents; a temperature control system configured to cycle the temperature of the sample; an excitation source for illuminating the sample; a fiber optic probe comprising (i) an optical fiber having a distal end and a proximal end and (ii) a probe head connected to the distal end of the optical fiber and positioned between the distal end of the optical fiber and the channel; and a detector configured to detect emissions exiting the proximal end of the optical fiber.
    Type: Application
    Filed: October 2, 2017
    Publication date: February 1, 2018
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventor: Kenton C. Hasson
  • Patent number: 9861985
    Abstract: The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: January 9, 2018
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Sami Kanderian, Gregory H. Owen, Hongye Liang, Scott Corey, Brian Bean
  • Patent number: 9829389
    Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, a microfluidic device is provided that includes a microfluidic chip having a plurality of microfluidic channels and a plurality of multiplexed heater electrodes, wherein the heater electrodes are part of a multiplex circuit including a common lead connecting the heater electrodes to a power supply, each of the heater electrodes being associated with one of the microfluidic channels. The microfluidic device also includes a control system configured to regulate power applied to each heater electrode by varying a duty cycle, the control system being further configured to determine the temperature of each heater electrode by determining the resistance of each heater electrode.
    Type: Grant
    Filed: February 22, 2016
    Date of Patent: November 28, 2017
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Gregory H. Owen, Gregory A. Dale
  • Patent number: 9823135
    Abstract: The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one aspect, the present invention provides a method for controlling the temperature of a heater electrode associated with a microfluidic channel of a microfluidic device, wherein power applied to the heater electrode is regulated by varying the duty cycle of a pulse width modulation (PWM). In another aspect, the present invention a controller configured to compute the temperature of the heater electrode during the power-on portion of the duty cycle and the during the power-off portion of the duty cycle and to adjust the duty cycle as necessary to achieve a desired temperature in the heater electrode.
    Type: Grant
    Filed: December 28, 2015
    Date of Patent: November 21, 2017
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Gregory H. Owen, Gregory A. Dale, Kenton C. Hasson
  • Publication number: 20170327867
    Abstract: The present invention relates to sample-to-answer systems, devices, cartridges, and method of using the same for detecting the presence of microorganisms in a sample, such as bacteria.
    Type: Application
    Filed: December 22, 2016
    Publication date: November 16, 2017
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Abhijit Dohale, Arvind Virmani, Brian Scrivens, Christopher Sneeder, Denis Alias, George Maltezos, Hanyoup Kim, Harini Shandilya, Hongye Liang, Jason Zsak, Johnathan Stuart Coursey, Kenton C. Hasson, Melissa Gosse, Shulin Zeng, Yasuyuki Numajiri, Makoto Ogusu, Yoichi Murakami, Kunihiro Sakai
  • Publication number: 20170325288
    Abstract: The present invention relates to methods and systems that result in high quality, reproducible, thermal melt analysis on a microfluidic platform. The present invention relates to methods and systems using thermal systems including heat spreading devices, including interconnection methods and materials developed to connect heat spreaders to microfluidic devices. The present invention also relates to methods and systems for controlling, measuring, and calibrating the thermal systems of the present invention.
    Type: Application
    Filed: January 23, 2017
    Publication date: November 9, 2017
    Applicant: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson
  • Patent number: 9777318
    Abstract: A system and method for amplifying and detecting nucleic acids are disclosed. In one embodiment, the system includes: a microfluidic device comprising a channel for receiving a sample of solution containing real-time PCR reagents; a temperature control system configured to cycle the temperature of the sample; an excitation source for illuminating the sample; a fiber optic probe comprising (i) an optical fiber having a distal end and a proximal end and (ii) a probe head connected to the distal end of the optical fiber and positioned between the distal end of the optical fiber and the channel; and a detector configured to detect emissions exiting the proximal end of the optical fiber.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: October 3, 2017
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventor: Kenton C. Hasson