Abstract: An apparatus for vacuum-sealing a vial includes a table for receiving the vial temporarily closed with a rubber stopper, a vertically movable enclosing device having a chamber at the lower end thereof for air-tightly enclosing a mouth of the vial in the chamber to hold the vial securely on the table when the enclosing device is lowered, a rubber stopper suction head vertically movable within the chamber of the enclosing device independently thereof, a first evacuating means adapted to communicate with the chamber so as to evacuate the vial, a second evacuating device adapted to communicate with the rubber stopper suction head, whereby, after the enclosing device is lowered to enclose the vial in the chamber, the rubber stopper suction head is lowered to suck the rubber stopper and is raised to remove the rubber stopper from the mouth of the vial so as to evacuate the vial, and the rubber stopper suction head is then again lowered to vacuum-seal the vial with the rubber stopper.