Abstract: The present application belongs to the technical field of biology. Provided is an application of lipid biomarkers in diagnosis and early warning of fatty liver in periparturient dairy cows. 20 lipid biomarkers found in the present disclosure have diagnostic values in recognizing, early warning, and diagnosing fatty liver diseases in dairy cows at corresponding disease stages. The lipid biomarkers of the present disclosure may be used for identifying and diagnosing the dairy cows with fatty liver.

Abstract: The present application belongs to the technical field of biology. Provided is exosome miRNA biomarkers in diagnosis and early warning of fatty liver in periparturient dairy cows. 12 miRNA biomarkers are discovered in the present application with diagnostic values in recognizing, early warning, identifying, and diagnosing fatty liver disease in dairy cows at corresponding disease stages, especially at least one of three of these miRNAs may be enriched without exosome extraction, and may be used to diagnose, in serum miRNA, whether the dairy cows is suffered from the disease.

Abstract: The present disclosure discloses the application of fecal microbiological markers in non-invasive identification/early warning of fatty liver in perinatal cows, belonging to the technical field of microorganisms and clinical medicine. The microbiological marker is a combination of one or more of Bifidobacteriumpseudolongum, Prevotellamultisaccharivorax and Lachnospiraceae bacterium.