Abstract: Methods of releasing sporocysts/sporozoites from oocysts are provided wherein a solution of oocysts is subjected to controlled shear forces sufficient to rupture the oocysts walls and release sporocysts/sporozoites therefrom. A solution of oocysts is passed through a Microfluidizer® processor chamber unit under defined conditions of chamber diameter, chamber geometry, and pressure. Oocysts impact the wall of the chamber and are subjected to controlled, high shear forces, tearing open the oocyst wall and releasing the sporocysts/sporozoites intact.