Abstract: Methods are provided for detecting and quantifying the DLL1, DLL3, JAG1, JAG2, Notch1, Notch 2, Notch 3, Notch 4, and/or DLL4 proteins in biological samples, such as a formalin fixed paraffin embedded tissue samples, using mass spectrometry. Additionally, methods are provided for treating cancer based upon the level of the DLL1, DLL3, JAG1, JAG2, Notch1, Notch 2, Notch 3, Notch 4, and/or DLL4 proteins in the biological samples.

Abstract: Methods are provided for quantifying specific proteins directly in biological samples that have been fixed in formalin by SRM/MRM assay. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks. A protein digest is prepared from the biological sample using, for example, the Liquid Tissue reagents and protocol and a designated protein is quantitated in the digest sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the described peptides. The proteins that can be detected and/or quantitated are CD3D, B7H3, B7-2, STAT1, GBP1, GPNMB, CD27, CD3E, and CD8.

Abstract: SRM/MRM assays are used to detect and quantitate proteins involved in the process of initiating, inhibiting, maintaining, and/or otherwise modulating a tumor immune response directly in patient tumor tissue. The assays provide an immune profile of the tissue microenvironment, and may be used as part of improved methods of immune-based treatment using agents that manipulate the cancer immune response together with cancer therapeutic agents.

Abstract: A molded component having a thickness reduction feature is provided. The molded component can include an injection molded polymer substrate having a front panel, a side panel, and a corner between and adjoining the front panel and the side panel. The substrate can also have a groove extending along the corner between the front panel and the side panel, the groove having a front panel edge and a side panel edge. The front panel edge and/or side panel edge have a dentil profile edge, the dentil profile edge reducing an effective thickness of the corner and thus providing increased or controlled cooling of the substrate.

Abstract: Methods are provided for quantifying CD56 and CHGA proteins directly in formalin-fixed biological samples by Selected Reaction Monitoring (SRM)/Multiple Reaction Monitoring (MRM) mass spectrometry. The biological samples may include formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, and FFPE tissue blocks and cells from those blocks. A protein sample may be prepared from said biological sample using the Liquid Tissue reagents and protocol and a designated protein is quantitated in the Liquid Tissue sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one peptide fragment derived from each of the proteins.

Abstract: Methods are provided herein for using SRM/MRM assays to detect and quantitate proteins involved in all cellular processes including cell division, cellular differentiation, cell growth inhibition, cellular metabolism, cell signaling, and tumor immune response/modulation in a protein digest prepared from a biological sample of formalin fixed tumor tissue. The SRM/MRM assays can provide a tumor tissue profile of the entire tissue microenvironment, regardless of cellular origin of expression, which can provide an optimal cancer therapy treatment.

Abstract: A vehicle console assembly includes a console housing including a storage compartment including a floor, a front wall, a rear wall and opposite side walls that extend between the front wall and the end wall defining a storage volume. A device charging tray includes a floor, a front wall, a rear wall and opposite side walls that extend between the front wall and the rear wall defining a storage volume. The device charging tray includes a raised support boss that extends out of the floor at the front wall. The raised support boss reduces a distance between the floor and an edge of the front wall. The raised support boss is located to support a mobile computing device positioned within the storage volume of the device charging tray spaced from the floor.

Abstract: A vehicle console assembly includes a console housing including a storage compartment including a floor, a front wall, a rear wall and opposite side walls that extend between the front wall and the end wall defining a storage volume. A device charging tray includes a floor, a front wall, a rear wall and opposite side walls that extend between the front wall and the rear wall defining a storage volume. The device charging tray includes a raised support boss that extends out of the floor at the front wall. The raised support boss reduces a distance between the floor and an edge of the front wall. The raised support boss is located to support a mobile computing device positioned within the storage volume of the device charging tray spaced from the floor.

Abstract: Methods are provided for quantifying specific proteins directly in biological samples that have been fixed in formalin by SRM/MRM assay. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks. A protein digest is prepared from the biological sample using, for example, the Liquid Tissue reagents and protocol and a designated protein is quantitated in the digest sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the described peptides. The proteins that can be detected and/or quantitated are CD3D, B7H3, B7-2, STAT1, GBP1, GPNMB, CD27, CD3E, and CD8.

Abstract: Methods are provided for quantifying specific proteins directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from said biological sample using the Liquid Tissue reagents and protocol and a designated protein is quantitated in the Liquid Tissue sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the described peptides.

Abstract: An adaptive speaker panel is provided. The speaker panel can have a speaker opening and a pair of concentric sealing ribs proximate to the speaker opening, the pair of sealing ribs having a first sealing rib and a second sealing rib towards a center of the speaker opening from the first sealing rib. The first sealing rib is first sealing rib dimensioned to seal against a first speaker basket and the second sealing rib dimensioned to seal against a second speaker basket that is smaller than the first speaker basket. Also, the first sealing rib and the second sealing rib are operatively arranged such that without modification to the speaker panel the first speaker basket or the second speaker basket can be attached to and sealed against the panel.

Abstract: A foam-filled panel is provided. The foam-filled panel can have a substrate layer with a padded area, an unpadded area, an opening within said unpadded area, and a bounding region between the padded area and the unpadded area. A skin can extend across the padded area of the substrate layer and a foam layer can be present between the padded area of the substrate layer and the skin. In addition, a disposable foam injection port is attached to the substrate layer at the bounding region of the padded area and can extend into and be an obstruction to the opening. The disposable foam injection port can be in fluid communication with the foam layer and unattaching the disposable foam injection port from the substrate layer can remove the obstruction to the opening.

Abstract: A foam filled panel with decorative thread stitching is provided. In addition, the stitching can be sealed to prevent leakage of foam when it is injected between a substrate and a skin layer to provide a foam filled panel. The decorative thread stitching can be sewn into the skin layer of the foam filled panel before the panel is assembled and filled with injectable foam. In addition, the skin can be made via slush molding, which allows various features and details to be molded into the skin. In some instances, the stitching can be sealed by adhesively attaching an impermeable membrane along a length of the stitching, thereby covering all the openings. In other instances, the stitching is sealed by using an expandable thread. Also disclosed is a process for manufacturing a foam filled panel with decorative thread stitching.

Abstract: The instrument panel assembly for an automotive vehicle includes an instrument panel and a component. The instrument panel includes an exterior surface and a cutout portion having a base which is recessed from the exterior surface. The cutout portion includes an inner wall extending between the base and the exterior surface of the instrument panel. The component attaches to the instrument panel. The component includes an engagement portion located at a distal end. The distal end of the engagement portion is at least partially received within the cutout portion of the instrument panel. The engagement of the distal end of the engagement portion of the component within the cutout portion of the instrument panel provides a continuous contact between the outer surface of the engagement portion and the inner wall of the cutout portion.

Abstract: A molded component having a thickness reduction feature is provided. The molded component can include an injection molded polymer substrate having a front panel, a side panel, and a corner between and adjoining the front panel and the side panel. The substrate can also have a groove extending along the corner between the front panel and the side panel, the groove having a front panel edge and a side panel edge. The front panel edge and/or side panel edge have a dentil profile edge, the dentil profile edge reducing an effective thickness of the corner and thus providing increased or controlled cooling of the substrate.

Abstract: A foam-filled panel is provided. The foam-filled panel can have a substrate layer with a padded area, an unpadded area, an opening within said unpadded area, and a bounding region between the padded area and the unpadded area. A skin can extend across the padded area of the substrate layer and a foam layer can be present between the padded area of the substrate layer and the skin. In addition, a disposable foam injection port is attached to the substrate layer at the bounding region of the padded area and can extend into and be an obstruction to the opening. The disposable foam injection port can be in fluid communication with the foam layer and unattaching the disposable foam injection port from the substrate layer can remove the obstruction to the opening.

Abstract: A foam filled panel with decorative thread stitching is provided. In addition, the stitching can be sealed to prevent leakage of foam when it is injected between a substrate and a skin layer to provide a foam filled panel. The decorative thread stitching can be sewn into the skin layer of the foam filled panel before the panel is assembled and filled with injectable foam. In addition, the skin can be made via slush molding, which allows various features and details to be molded into the skin. In some instances, the stitching can be sealed by adhesively attaching an impermeable membrane along a length of the stitching, thereby covering all the openings. In other instances, the stitching is sealed by using an expandable thread. Also disclosed is a process for manufacturing a foam filled panel with decorative thread stitching.

Abstract: An adaptive speaker panel is provided. The speaker panel can have a speaker opening and a pair of concentric sealing ribs proximate to the speaker opening, the pair of sealing ribs having a first sealing rib and a second sealing rib spaced inwardly from the first scaling rib. The first and second sealing ribs are arranged such that without modification to the speaker panel, a speaker basket can seal against the first sealing rib if the basket is too large to seal against the second sealing rib, or in the alternative, the speaker basket can seal against the second sealing rib if the basket is too small to seal against the first sealing rib.

Abstract: The instrument panel assembly for an automotive vehicle includes an instrument panel and a component. The instrument panel includes an exterior surface and a cutout portion having a base which is recessed from the exterior surface. The cutout portion includes an inner wall extending between the base and the exterior surface of the instrument panel. The component attaches to the instrument panel. The component includes an engagement portion located at a distal end. The distal end of the engagement portion is at least partially received within the cutout portion of the instrument panel. The engagement of the distal end of the engagement portion of the component within the cutout portion of the instrument panel provides a continuous contact between the outer surface of the engagement portion and the inner wall of the cutout portion.

Abstract: The present invention enables desktop applications to access web services through Plug-ins and a Web Service Proxy Server. An administrator registers a web service by providing the URL of the WSDL file of the web service. The target desktop applications and the operations are identified using the WSDL file. Operations that are not compatible with the desktop applications are removed from a published list of operations. The administrator appends additional formatting information, communication standards and security policies to the WSDL file. A user accessing the web services is first authenticated and authorized. Thereafter, the user accesses the web services through Web Service Proxy Server. The communication with the web services complies with the standards and security policies specified in the WSDL files. The output data obtained from the web services are presented using template documents. These template documents are generated based on the formatting information provided in the WSDL files.