Abstract: A connection device between two micro-channels or two milli-channels of two fluidic elements including a complementary arrangement for holding the device with at least one of the two fluidic elements. A micro-fluidic or milli-fluidic chip including a connection device between a micro-channel or a milli-channel of the chip and a micro-channel or a milli-channel of a fluidic element.

Abstract: The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

Abstract: The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

Abstract: The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.

Abstract: The invention relates to a method for in vitro characterization of a eukaryote biological microtissue by imaging by means of a phase measurement technique without a reference beam, and the use of this method in vitro characterization in particular for: monitoring the quality of a microtissue, measuring the increase of the biomass of a microtissue during its growth and/or its amplification following the differentiation and/or the organization of a microtissue during its maturation, determining the phenotype of cells of the microtissue, and/or confirming the absence of non-differentiated cells in the microtissue.

Abstract: The invention relates to a process for producing a plurality of capsules, each comprising an external shell of crosslinked hydrogel surrounding a central core, according to which a hydrogel solution and a composition of interest, intended to form the central core, are concentrically co-extruded so as to form mixed drops, each comprising a layer of hydrogel solution surrounding a drop of liquid composition of interest, characterized in that the co-extrusion step is carried out above a crosslinking aerosol so that the mixed drops pass through said crosslinking aerosol, such that the layer of hydrogel solution at least partially crosslinks around the drop of liquid composition of interest on contact with said aerosol.

Abstract: The present invention relates to a method for in-vitro production of mammalian neurons expressing the 6 isoforms of the Tau protein (2N4R, 1N4R, 0N4R, 2N3R, 1N3R, 0N3R), comprising a step of neuronal differentiation, in which cellular microcompartments are cultivated for a period of 5 weeks to 100 weeks, each one comprising a hollow hydro gel capsule surrounding post-mitotic neuronal cells and an extracellular matrix, the neuronal differentiation step being carried out in a bioreactor, the cellular microcompartments being kept in suspension in an enclosure of the bioreactor containing a neuronal differentiation medium.

Abstract: The invention relates to three-dimensional tissue units which are hollow and which comprise, when organized about an internal opening, at least one layer of living human retinal pigment epithelium cells which are differentiated, the basal face of each cell pointing outwards and the apical face pointing towards the internal opening. The invention also relates to these tissue units for use in the treatment of retinopathies, and to a method for preparing these tissue units and an implantation kit.

Abstract: The invention relates to a bioreactor cell culture system comprising a closed chamber containing a plurality of suspended cell microcompartments, wherein the microcompartments each comprise an outer hydrogel layer providing a cavity containing a set of self-organized cells and extracellular matrix or an extracellular matrix substitute. The invention further relates to the use of such bioreactors to produce cells and/or organoids, and/or molecules and/or complex molecular assemblies.

Abstract: The invention relates to a process for preparing cellular microcompartments comprising a hydrogel capsule surrounding a cluster of lymphomatous cells. The invention also relates to such a cellular microcompartment and the use thereof for screening anti-cancer molecules.

Abstract: The invention relates to a process for producing a plurality of capsules, each comprising an external shell of crosslinked hydrogel surrounding a central core, according to which a hydrogel solution and a composition of interest, intended to form the central core, are concentrically co-extruded so as to form mixed drops, each comprising a layer of hydrogel solution surrounding a drop of liquid composition of interest, characterized in that the co-extrusion step is carried out above a crosslinking aerosol so that the mixed drops pass through said crosslinking aerosol, such that the layer of hydrogel solution at least partially crosslinks around the drop of liquid composition of interest on contact with said aerosol.

Abstract: A capsule containing at least one mammalian cell, includes a liquid core, and at least one external envelope totally encapsulating the liquid core at its periphery, the external envelope including at least one gelled polyelectrolyte and/or a stiffened biopolymer and being able to retain the liquid core when the capsule is immersed in a gas. The present invention further relates to the method for preparing such a capsule, to a method for screening cosmetic active ingredients as well as a culture method using such capsules.

Abstract: The invention relates to a neural tissue unit for use in implantation into the nervous system of a human or non-human mammal, wherein said neural tissue unit contains differentiated post-mitotic neuronal cells in an extracellular matrix, said unit being obtained from a cellular microcompartment comprising a hydrogel capsule surrounding the neural tissue unit, and said hydrogel capsule being at least partially removed before use of the neural tissue unit. The invention also relates to a process for preparing such a neural tissue unit.

Abstract: The invention relates to a hollow cell microfibre comprising successively, organized around a lumen, at least one endothelial cell layer, at least one smooth muscle cell layer, an extracellular matrix layer, and optionally an outer hydrogel layer. The invention also relates to a process for fabricating such a hollow cell microfibre.

Abstract: The invention relates to a cellular microcompartment comprising successively, organized around a lumen, at least one layer of pluripotent cells, an extracellular matrix layer and an outer hydrogel layer. The invention also relates to processes for preparing such cellular microcompartments.