Abstract: Provided herein are methods for design of engineered polypeptides that recapitulate molecular structure features of a predetermined portion of several reference protein structures, e.g., related antibody epitopes or protein binding sites. A Machine Learning (ML) model may be used to generate engineered polypeptides. After substituting target residues for corresponding residues in other reference structures in the same scaffold, the scaffolds with each set of target residues may be filtered by structural comparison to identify generalized scaffolds. Generalized scaffolds may be improved by screening libraries of polypeptides.

Abstract: A bag for carrying tools and a multitude of accessories is disclosed. The bag includes an open top, a fabric bottom panel, two spaced, rigid, fabric covered end panels, and two spaced fabric side panels as well as two carrying straps and a handle. At least one tool mat for storing small tools and accessories extends from the bottom of the tool bag and attaches to one of the panels for versatile and organized tool storage.