Abstract: A novel antigenic protein which is effective for stimulating antibody production in animals against the sheep scab mite, Psoroptes ovis is disclosed. In cattle, administration of the protein provides protection from infestation by P. ovis by eliciting an immediate-type hypersensitivity response and/or immunizing the animal against P. ovis. The protein is also effective for producing and/or binding antibodies to P. ovis, and may be used as an immunodiagnostic reagent.

Abstract: A novel antigenic protein which is effective for stimulating antibody production in animals against the sheep scab mite, Psoroptes ovis is disclosed. In cattle, administration of the protein provides protection from infestation by P. ovis by eliciting an immediate-type hypersensitivity response and/or immunizing the animal against P. ovis. The protein is also effective for producing and/or binding antibodies to P. ovis, and may be used as an immunodiagnostic reagent.

Abstract: Murine hybridomas are disclosed which were constructed by fusing spleen cells from BALB/c mice immunized with soluble crystal protein from Bacillus thuringiensis subsp. israelensis (B.t.i.) to the murine myeloma cell line SP2/0-AG14. An ELISA (enzyme-linked immunosorbent assay) method for detection of antibodies specific for crystal protein of B.t.i. was modified to produce 100- to 1,000-fold increased sensitivity and was used to identify hybridomas secreting monoclonal antibody specific for the B.t.i. crystal protein. Analysis of the hybridoma culture supernatant fluid indicated production of monoclonal IgG3 antibodies, specific for the 68,000 dalton protein presumed to be the insecticidal delta-endotoxin of B.t.i.