Abstract: Oligonucleotide primers and methods of use in producing sequencing libraries are provided according to aspects of the present disclosure which include, from 5? to 3?, a homopolymer-hybridizing region, and an anchor region comprising 5?-(?)nNm-3?, wherein the homopolymer-hybridizing region is a contiguous sequence of 5 to 20 elements, wherein the elements are nucleotides or Tm increasing nucleotide analogs, wherein at least 4 of the elements are Tm increasing nucleotide analogs, wherein the homopolymer-hybridizing region hybridizes to a complementary homopolymer tract of a target nucleic acid, wherein the complementary homopolymer tract comprises a contiguous sequence of complementary elements, wherein X is any nucleotide or nucleotide analog with the proviso that ? is not a nucleotide or nucleotide analog complementary to a complementary element of the complementary homopolymer tract, and wherein N is any nucleotide or nucleotide analog.

Abstract: Methods of reducing adapter-dimers in a sequencing library are provided according to aspects of the present disclosure, including: hybridizing excess 3? adapters to blocker oligonucleotides forming a hybridized complex, such that the excess 3? adapters are unavailable to form adapter-dimers, the hybridized complex including: a first blocker oligonucleotide 3? terminus adjacent to an adenylated nucleotide at the 5? terminus of a first unligated 3? adapter and a second blocker oligonucleotide 3? terminus adjacent to an adenylated nucleotide at the 5? terminus of a second unligated 3? adapter, wherein the first blocker oligonucleotide has a 5? portion complementary to and hybridized to the first unligated 3? adapter, the second blocker oligonucleotide has a 5? portion complementary to and hybridized to the second unligated 3? adapter, and the first blocker oligonucleotide has a 3? portion complementary to, and hybridized to, a 3? portion of the second blocker oligonucleotide.

Abstract: Oligonucleotide primers and methods of use in producing sequencing libraries are provided according to aspects of the present disclosure which include, from 5? to 3?, a homopolymer-hybridizing region, and an anchor region comprising 5?-(?)nNm-3?, wherein the homopolymer-hybridizing region is a contiguous sequence of 5 to 20 elements, wherein the elements are nucleotides or Tm increasing nucleotide analogs, wherein at least 4 of the elements are Tm increasing nucleotide analogs, wherein the homopolymer-hybridizing region hybridizes to a complementary homopolymer tract of a target nucleic acid, wherein the complementary homopolymer tract comprises a contiguous sequence of complementary elements, wherein ? is any nucleotide or nucleotide analog with the proviso that ? is not a nucleotide or nucleotide analog complementary to a complementary element of the complementary homopolymer tract, and wherein N is any nucleotide or nucleotide analog.

Abstract: The current teachings relate to methods for reducing adapter-dimer formation, particularly when preparing nucleic acids of interest for subsequent amplification and/or sequencing. Also described are kits for use in performing certain disclosed methods.

Abstract: The current teachings relate to methods for reducing adapter-dimer formation, particularly when preparing nucleic acids of interest for subsequent amplification and/or sequencing. Also described are kits for use in performing certain disclosed methods.

Abstract: The current teachings relate to methods for reducing adapter-dimer formation, particularly when preparing nucleic acids of interest for subsequent amplification and/or sequencing. Also described are kits for use in performing certain disclosed methods.