Abstract: A bioreactor can include: a tissue culture chamber; at least one inlet port into the tissue culture chamber; an inlet port member located in each inlet including an inlet tube extending into the tissue culture chamber; at least one outlet port into the tissue culture chamber; an outlet port member located in each outlet including an outlet tube extending into the tissue culture chamber; an optical cover; and a hydrogel can be located in the tissue culture chamber having at least one lumen fluidly coupling the inlet tube to the outlet tube, wherein an inlet interface region of the hydrogel is constrained around the inlet tube and an outlet interface region of the hydrogel is constrained around the outlet tube.

Abstract: A method of creating a reperfusion injury can include: providing a cell culture device having an internal chamber with at least one port coupled to a perfusion modulating system capable of modulating perfusion in the internal chamber, wherein the internal chamber includes a cell culture; perfusing a fluid through the internal chamber with the perfusion modulating system, wherein the perfusion modulating system includes at least one pump; reducing fluid flow through the internal chamber; reperfusing fluid flow through the internal chamber; and creating a reperfusion injury in the cell culture by the reperfusion of the fluid flow through the internal chamber. The cell culture includes at least one type of tissue cell. The cell culture can include a tissue construct formed of hydrogel and/or extracellular matrix.

Abstract: A method of creating a reperfusion injury can include: providing a cell culture device having an internal chamber with at least one port coupled to a perfusion modulating system capable of modulating perfusion in the internal chamber, wherein the internal chamber includes a cell culture; perfusing a fluid through the internal chamber with the perfusion modulating system, wherein the perfusion modulating system includes at least one pump; reducing fluid flow through the internal chamber; reperfusing fluid flow through the internal chamber; and creating a reperfusion injury in the cell culture by the reperfusion of the fluid flow through the internal chamber. The cell culture includes at least one type of tissue cell. The cell culture can include a tissue construct formed of hydrogel and/or extracellular matrix.