Abstract: A microwell array configured for high-throughput screening of micro-tissue and methods of using the same to prepare micro-tissue is disclosed. The microwell array includes a substrate having a plurality of wells, at least one longitudinal recess arranged in the bottom surface of each of the plurality of wells, and at least one micropillar arranged within the longitudinal recess at each end of the longitudinal recess. The microwell array advantageously can provide for high-throughput screening by enabling in vitro generation of three-dimensional micro-tissues that are accurate models of heart, skeletal muscle, neuronal, and other tissues in a device compatible with existing robotic liquid handlers to load cells into the devices, perform routine media changes, and add molecular probes and compounds when desired.

Abstract: The present disclosure provides methods of treating an ocular disease or disorder. The methods involve direct administration into the eye of a conjugate comprising a biologically active polypeptide and a biocompatible polymer.

Abstract: The invention relates to devices, methods, kits, and compositions for in vitro generation of three-dimensional micro-tissues that are accurate models of heart, skeletal muscle, neuronal, and other tissues.

Abstract: The present disclosure provides methods of treating an ocular disease or disorder. The methods involve direct administration into the eye of a conjugate comprising a biologically active polypeptide and a biocompatible polymer.

Abstract: The invention relates to devices, methods, kits, and compositions for in vitro generation of three-dimensional micro-tissues that are accurate models of heart, skeletal muscle, neuronal, and other tissues.

Abstract: The present disclosure provides methods of treating an ocular disease or disorder. The methods involve direct administration into the eye of a conjugate comprising a biologically active polypeptide and a biocompatible polymer.

Abstract: The present disclosure is directed to peptide-polymer conjugates, and to their use in treating intra-articular diseases or disorders.

Abstract: The invention relates to devices, methods, kits, and compositions for in vitro generation of three-dimensional micro-tissues that are accurate models of heart, skeletal muscle, neuronal, and other tissues.

Abstract: The present disclosure provides methods of treating an ocular disease or disorder. The methods involve direct administration into the eye of a conjugate comprising a biologically active polypeptide and a biocompatible polymer.

Abstract: The present invention provides materials and methods that can serve as a prosthetic and/or, for tissue engineering applications, as a supporting matrix in the stabilization of an injured or defective myocardial wall in a patient comprising injecting a polymer network at a pre-selected site in the myocardial wall of the patient.

Abstract: The present disclosure provides a 3-dimensional filamentous fiber matrix, systems comprising the matrix, and methods for using the matrix and the systems.

Abstract: The present disclosure provides a microfluidics device for culturing cells, such as cardiomyocytes or cardiomyocyte progenitors; and methods of culturing cells using the device. The device and culturing methods find use in drug screening methods, for methods of evaluating a drug under development, and for methods of predicting patient response to a given treatment regimen, which methods are also provided.

Abstract: Multi-organ cell culture systems and methods are provided. Aspects of the cell culture systems include at least two microfluidic cell culture units configured to culture a plurality of cells, one or more connectors configured to fluidly connect the microfluidic cell culture units to one another, a cell culture medium configured to support the growth of a plurality of different cell types, and a controller configured to move the cell culture medium at a specified volumetric flow rate between the microfluidic cell culture units. The subject systems and methods find use in a variety of applications, including in vitro evaluation of candidate agents for toxicity and efficacy, in vitro models of disease, and in vitro models for fundamental studies of biological systems.

Abstract: The present disclosure provides compositions comprising a hydrogel and a cell adhesion ligand that enhances the differentiation of mesenchymal stem cells into brown adipocytes, and/or enhances the maintenance of brown adipocytes. In some cases, a subject composition includes cells that are embedded within the hydrogel. The present disclosure also provides methods of making and using the subject compositions.

Abstract: The present disclosure provides methods of treating an ocular disease or disorder. The methods involve direct administration into the eye of a conjugate comprising a biologically active polypeptide and a biocompatible polymer.

Abstract: The present disclosure provides methods for sulfonation of hyaluronic acid. The present disclosure provides sulfonated hyaluronic acid, and compositions, including pharmaceutical compositions, comprising the sulfonated hyaluronic acid. The present disclosure provides implantable materials and drug delivery compositions comprising a subject sulfonated hyaluronic acid.

Abstract: Provided herein are hydrogel cell matrices, hydrogel cell matrix systems for the support, growth, and differentiation of a stem cell or progenitor cell and methods for making such hydrogel cell matrices.

Abstract: The invention relates to devices, methods, kits, and compositions for in vitro generation of three-dimensional micro-tissues that are accurate models of heart, skeletal muscle, neuronal, and other tissues.

Abstract: The present invention provides materials and methods that can serve as a prosthetic and/or, for tissue engineer applications, as a supporting matrix in the stabilization of the myocardium.

Abstract: Provided herein are compositions and methods for isolating and culturing cardiac progenitor cells, and for improved transplantation.