Abstract: Methods of reducing microbial attachment to a surface are provided, including methods comprising illuminating a surface comprising a substrate and a coating on the substrate with ultraviolet light, wherein the coating comprises anatase titanium dioxide nanoparticles functionalized with silver nanoparticles and is optically transparent to visible light; and exposing the illuminated surface to microbes. The coating exhibits a reduction in microbial attachment as compared to the coating absent the illumination.

Abstract: Systems, devices, and methods for localizing magnets are useful for biological tissue analysis and other applications. Tissue analysis systems for use with a tissue analysis device may include a storage medium storing computer readable instructions that cause the execution of magnet localization methods. The system may include the tissue analysis device, which includes a magnet and a magnetometer array disposed adjacent to the magnet. When executed by a processor, the instructions cause operations, including recording a magnetic field of the magnet with the magnetometer array, simulating a simulated magnetic field at each magnetometer of the magnetometer array, and estimating positional information of the magnet based upon iterating simulated positional information of the simulated magnet.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: A system and method for performing service actions on a vehicle according to a ruleset. The ruleset classifies serviceable conditions of the vehicle according to rules defined within the ruleset. A diagnostic tool is utilized to perform the service actions and is configured to establish data communication with other diagnostic devices that the rules indicate may be required for a certified service action. After the completion of the service actions, certified repair results are generated indicating successful completion of the service action.

Abstract: A system and method for performing service actions on a vehicle according to a ruleset. The ruleset classifies serviceable conditions of the vehicle according to rules defined within the ruleset. A diagnostic tool is utilized to perform the service actions and is configured to establish data communication with other diagnostic devices that the rules indicate may be required for a certified service action. After the completion of the service actions, certified repair results are generated indicating successful completion of the service action.

Abstract: Methods and systems for generating three-dimensional (3D) engineered tissues (ETs), and for electrical stimulation of same, are provided. Provided is an ET assembly comprising an ET lid with first post and second post assemblies coupled thereto. Provided is a casting assembly comprising the ET assembly and a casting plate. Provided are stimulation methods and systems for stimulating tissue constructs.

Abstract: This invention relates to the isolation and purification of nutritional supplements and prebiotics, such as xylo-oligosaccharides, and specifically the removal of color bodies, said xylo-oligosaccharides sourced from from ligno-cellulosic feedstocks such as sugar cane and sugar cane bagasse. Removal of color bodies provides greater purity in certain xylo-oligosaccharides as described. This process can also be applied to non-sugar cane feedstocks such as agricultural residues (wheat straw, corn stover, rice straw, etc.), purpose grown crops including but not limited to sugar cane, sorghum, Miscanthus, and woody biomass such as poplar.

Abstract: This disclosure relates to the co-production and co-delivery of a pre- and postbiotic and antioxidant that are chemically linked and thus can be delivered as a single product instead of a blend of individual molecules. This xylooligosaccharide (XOS) product is used as a nutritional supplement and/or food ingredient that improves digestive health and overall wellness.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: An electric machine is described together with a stator. The electric machine comprises a stator, said stator comprising a cylindrical stator core having an end face; slots provided on the end face, each slot running through the stator core; a plurality of conductor bars disposed within the slots; and an end face assembly electrically connecting at least two of the conductor bars; a rotor having a plurality of magnetic pole pairs; and a controller electrically connected to said stator for regulating an excitation current supplied to or from the conductor bars, wherein the controller regulates an amplitude of the excitation current independently of a frequency of the excitation current. This arrangement simplifies stator construction and allows for optimisation of the electric machine.

Abstract: Topographically-guided cell migration devices include a substrate, an ion-permeable surface layer having a unidirectional textured first side, and an electrode pair having interdigitated electrode digits disposed between the substrate and the ion-permeable surface layer. The unidirectional texture of the ion-permeable surface layer is configured to guide migration of a cell culture in a substantially linear migration direction. Other topographically-guided cell migration devices include a substrate, a surface layer having a unidirectional textured first side, and a cell confinement structure with two walls that run substantially parallel to the unidirectional texture in order to guide migration of the cell culture in a substantially linear migration direction.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.

Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. In one aspect, the polypeptides of the invention can be used as amylases, for example, alpha amylases, to catalyze the hydrolysis of starch into sugars. In one aspect, the invention provides delayed release compositions comprising a desired ingredient coated by a latex polymer coating.

Abstract: An apparatus for biological analysis includes a substrate, and an ion-permeable material with a textured surface on a first side of the ion-permeable material. The substrate is disposed proximate to a second side of the ion-permeable material, opposite the first side. A plurality of electrodes is disposed between the substrate and the ion-permeable material, where individual electrodes in the plurality of electrodes are positioned to measure an electrical signal that passes through the ion-permeable material.

Abstract: An electric machine is described together with a stator. The electric machine comprises a stator, said stator comprising a cylindrical stator core having an end face; slots provided on the end face, each slot running through the stator core; a plurality of conductor bars disposed within the slots; and an end face assembly electrically connecting at least two of the conductor bars; a rotor having a plurality of magnetic pole pairs; and a controller electrically connected to said stator for regulating an excitation current supplied to or from the conductor bars, wherein the controller regulates an amplitude of the excitation current independently of a frequency of the excitation current. This arrangement simplifies stator construction and allows for optimisation of the electric machine.

Abstract: In one aspect, the invention is directed to polypeptides having an amylase activity, polynucleotides encoding the polypeptides, and methods for making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used as amylases to catalyze the hydrolysis of starch into sugars.

Abstract: The invention relates to alpha amylases and to polynucleotides encoding the alpha amylases. In addition, methods of designing new alpha amylases and methods of use thereof are also provided. The alpha amylases have increased activity and stability at acidic, neutral and alkaline pH and increased temperature.

Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.