Abstract: Tissue modulating systems, devices, and methods modulate and/or stimulate numerous tissue specimens, independently and in parallel, within a multi-well plate in such a way to allow simultaneous measurement of functional properties of the tissue specimens. Tissue modulating system includes a first plurality of tissue fixtures, with at least one of the first tissue fixtures extending into each well. The system also includes a tissue fixture modulating apparatus including a second plurality of tissue fixtures, with at least one of the second tissue fixtures extending into each well. The tissue fixture modulating apparatus displaces the second tissue fixture in each well.

Abstract: A system and method for performing service actions on a vehicle according to a ruleset. The ruleset classifies serviceable conditions of the vehicle according to rules defined within the ruleset. A diagnostic tool is utilized to perform the service actions and is configured to establish data communication with other diagnostic devices that the rules indicate may be required for a certified service action. After the completion of the service actions, certified repair results are generated indicating successful completion of the service action.

Abstract: A flow system is used to mix and transport a slurry of sand and water in support of a hydraulic fracturing operation that is to be conducted at a well site location. The flow system utilizes lay-flat hose to transport the slurry under motive force provided by one or more centrifugal pumps. The lay-flat hose may be constructed in total lengths exceeding several miles in length and may be provided to place a mobile mine in fluidic communication with the well site location.

Abstract: An automated gate actuator system is provided for use in selectively opening and closing a gate that dispenses proppant from a proppant container during the course of a hydraulic fracturing operation. A lever arm is pivotally mounted for movement to place a drive gear in contact with a driven gear to open and close the gate. The gate may be remotely or autonomously actuated in this manner utilizing an electronic control triggered by human and/or sensor input.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: Systems, devices, and methods for localizing magnets are useful for biological tissue analysis and other applications. Tissue analysis systems for use with a tissue analysis device may include a storage medium storing computer readable instructions that cause the execution of magnet localization methods. The system may include the tissue analysis device, which includes a magnet and a magnetometer array disposed adjacent to the magnet. When executed by a processor, the instructions cause operations, including recording a magnetic field of the magnet with the magnetometer array, simulating a simulated magnetic field at each magnetometer of the magnetometer array, and estimating positional information of the magnet based upon iterating simulated positional information of the simulated magnet.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: An automated gate actuator system is provided for use in selectively opening and closing a gate that dispenses proppant from a proppant container during the course of a hydraulic fracturing operation. A lever arm is pivotally mounted for movement to place a drive gear in contact with a driven gear to open and close the gate. The gate may be remotely or autonomously actuated in this manner utilizing an electronic control triggered by human and/or sensor input.

Abstract: Various aspects of the subject technology relate to systems, methods, and machine-readable media for providing open-ended input data and prompting a response to solicit additional information from a user. A method comprises receiving initial input data. The method includes determining a plurality of categories associated with the initial input data; determining for each category in the plurality of categories, a threshold value and a category likeliness score value; and determining a category match between a category and the input data, where the category is one of a plurality of categories, by calculating when the likeliness score value exceeds the threshold value. The method further includes providing a probe request associated with each category match and determining a prediction score associated with each probe request.

Abstract: Systems, devices, and methods for localizing magnets are useful for biological tissue analysis and other applications. Tissue analysis systems for use with a tissue analysis device may include a storage medium storing computer readable instructions that cause the execution of magnet localization methods. The system may include the tissue analysis device, which includes a magnet and a magnetometer array disposed adjacent to the magnet. When executed by a processor, the instructions cause operations, including recording a magnetic field of the magnet with the magnetometer array, simulating a simulated magnetic field at each magnetometer of the magnetometer array, and estimating positional information of the magnet based upon iterating simulated positional information of the simulated magnet.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: A system and method for performing service actions on a vehicle according to a ruleset. The ruleset classifies serviceable conditions of the vehicle according to rules defined within the ruleset. A diagnostic tool is utilized to perform the service actions and is configured to establish data communication with other diagnostic devices that the rules indicate may be required for a certified service action. After the completion of the service actions, certified repair results are generated indicating successful completion of the service action.

Abstract: A system and method for performing service actions on a vehicle according to a ruleset. The ruleset classifies serviceable conditions of the vehicle according to rules defined within the ruleset. A diagnostic tool is utilized to perform the service actions and is configured to establish data communication with other diagnostic devices that the rules indicate may be required for a certified service action. After the completion of the service actions, certified repair results are generated indicating successful completion of the service action.

Abstract: Methods and systems for generating three-dimensional (3D) engineered tissues (ETs), and for electrical stimulation of same, are provided. Provided is an ET assembly comprising an ET lid with first post and second post assemblies coupled thereto. Provided is a casting assembly comprising the ET assembly and a casting plate. Provided are stimulation methods and systems for stimulating tissue constructs.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.

Abstract: An electric machine is described together with a stator. The electric machine comprises a stator, said stator comprising a cylindrical stator core having an end face; slots provided on the end face, each slot running through the stator core; a plurality of conductor bars disposed within the slots; and an end face assembly electrically connecting at least two of the conductor bars; a rotor having a plurality of magnetic pole pairs; and a controller electrically connected to said stator for regulating an excitation current supplied to or from the conductor bars, wherein the controller regulates an amplitude of the excitation current independently of a frequency of the excitation current. This arrangement simplifies stator construction and allows for optimisation of the electric machine.

Abstract: Topographically-guided cell migration devices include a substrate, an ion-permeable surface layer having a unidirectional textured first side, and an electrode pair having interdigitated electrode digits disposed between the substrate and the ion-permeable surface layer. The unidirectional texture of the ion-permeable surface layer is configured to guide migration of a cell culture in a substantially linear migration direction. Other topographically-guided cell migration devices include a substrate, a surface layer having a unidirectional textured first side, and a cell confinement structure with two walls that run substantially parallel to the unidirectional texture in order to guide migration of the cell culture in a substantially linear migration direction.

Abstract: Proteins, nucleic acids encoding the proteins, compositions comprising the proteins, and methods are provided. The proteins have the ability to be self-targeted to ICAM-1 and, if desired, enzymatically-released at acidic pH. The ICAM-1-targeting peptides are provided as single copies or multiples repeats, and can be separated by linkers from the enzyme segment, from which the ICAM-1 targeting peptides can be released, if desired, at acidic pH. These fusion proteins enhance the activity of the enzyme segment within or liberated from the fusion protein, and provide increased recognition and targeting of diseased organs, transport from the bloodstream across the endothelium into said diseased organ, and intracellular uptake and lysosomal trafficking by cells in them, both in peripheral tissues and the central nervous system. Representative nucleotide and amino acid sequences of these fusion proteins, as well as in vitro, cellular, and in vivo animal data are provided.