Abstract: Embodiments of the present disclosure provide improved methods and apparatus for coupling a probe to an instrument device manipulator (e.g., robotic arm). In some embodiments, a probe, which may be carried by a handpiece, is configured to couple to the end of a device manipulator of a robotic arm. A transmission can be provided that is configured to couple to the probe and also couple to the instrument device manipulator. The transmission can be configured to receive motion input from the instrument device manipulator and transfer the motion input into motion of the probe. The transmission can provide improved movement of the probe. By decreasing the number of components on the handpiece and probe, the handpiece and probe can be provided as a sterile, single use consumable device and the transmission can be reused.

Abstract: A novel gRNA-ligand binding complex is provided. This complex may be used to bring Type V Cas proteins and additional effectors to DNA for base editing. The design of the systems allows for the production of efficient modular components that provide flexibility when editing DNA.

Abstract: A novel gRNA-ligand binding complex is provided. This complex may be used to bring Type V Cas proteins and additional effectors to DNA for base editing. The design of the systems allows for the production of efficient modular components that provide flexibility when editing DNA.

Abstract: The present invention discloses an RNA scaffold comprising a tracrRNA; and a recruiting RNA motif with an extension sequence for targeted gene editing and related uses. The method enables precise modifications to be made to the genome whilst minimizing the possibility of off-target effects, making the method particularly suitable for therapeutic applications.