Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Abstract: A system enables tool to process remote log files as if the log files were located locally. The system relies on technology, such a file system in user space (FUSE), to add log file search results to the local file system. In some embodiments, the log file search results are associated with virtual files in the local file system. A remote data store system communicates with the local system to provide the search results in response to a search query.

Abstract: A system enables tool to process remote log files as if the log files were located locally. The system relies on technology, such a file system in user space (FUSE), to add log file search results to the local file system. In some embodiments, the log file search results are associated with virtual files in the local file system. A remote data store system communicates with the local system to provide the search results in response to a search query.

Abstract: The invention provides a method for generating a database of candidate essential genes in Staphylococcus aureus, as well as otherwise important genes that, when mutated, lead to a growth attenuated phenotype. Such genes and mutants of such genes are important for identifying antibacterial agents suitable for treating and preventing S. aureus infections. The invention includes methods for confirming the essentiality or importance of candidate genes, as well as methods for utilizing those genes to screen for new antibacterial drugs. The invention also includes the antibacterial agents identified using the disclosed methods, as well as methods of using the same for treating and preventing Staphylococcus infection.

Abstract: A water gun may comprise a body having a front surface, a nozzle mounted on and extending from the body, an actuator, at least one fluid reservoir, and a pump. The nozzle may have at least a nozzle portion adapted to move between an extended position spaced away from the front surface and a retracted position closer to the front surface than the extended position. The actuator may be adapted to be moved relative to the body, and mechanically coupled to the nozzle for moving the nozzle between the retracted and extended positions when the actuator is moved between first and second positions. The fluid reservoir may comprise first and second end portions and a generally uniform elongate intermediate portion extending between the first and second end portions, which may have volumes that are larger than the volume of the intermediate portion.

Abstract: The invention includes a database of candidate essential genes in Pseudomonas aeruginosa, as well as other important genes that, when mutated, lead to a growth attenuated phenotype. Such genes and mutants of such genes are important for identifying antibacterial agents suitable for treating and preventing Pseudomonas aeruginosa infections. The invention includes methods for confirming the essentiality or importance of candidate genes, as well as methods for utilizing those genes to screen for new antibacterial drugs. The invention also includes the antibacterial agents identified using the disclosed methods, as well as methods of using the same for treating and preventing Pseudomonas infection.

Abstract: The invention includes a database of candidate essential genes in Pseudomonas aeruginosa, as well as other important genes that, when mutated, lead to a growth attenuated phenotype. Such genes and mutants of such genes are important for identifying antibacterial agents suitable for treating and preventing Pseudomonas aeruginosa infections. The invention includes methods for confirming the essentiality or importance of candidate genes, as well as methods for utilizing those genes to screen for new antibacterial drugs. The invention also includes the antibacterial agents identified using the disclosed methods, as well as methods of using the same for treating and preventing Pseudomonas infection.

Abstract: The invention provides a method for generating a database of candidate essential genes in Staphylococcus aureus, as well as otherwise important genes that, when mutated, lead to a growth attenuated phenotype. Such genes and mutants of such genes are important for identifying antibacterial agents suitable for treating and preventing S. aureus infections. The invention includes methods for confirming the essentiality or importance of candidate genes, as well as methods for utilizing those genes to screen for new antibacterial drugs. The invention also includes the antibacterial agents identified using the disclosed methods, as well as methods of using the same for treating and preventing Staphylococcus infection.

Abstract: A water gun may comprise a body having a front surface, a nozzle mounted on and extending from the body, an actuator, at least one fluid reservoir, and a pump. The nozzle may have at least a nozzle portion adapted to move between an extended position spaced away from the front surface and a retracted position closer to the front surface than the extended position. The actuator may be adapted to be moved relative to the body, and mechanically coupled to the nozzle for moving the nozzle between the retracted and extended positions when the actuator is moved between first and second positions. The fluid reservoir may comprise first and second end portions and a generally uniform elongate intermediate portion extending between the first and second end portions, which may have volumes that are larger than the volume of the intermediate portion.