Patents by Inventor Kevin J. McKernan
Kevin J. McKernan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220098637Abstract: A method for determining a level of one or more live microbes in a sample of a Cannabis plant includes (a) introducing a DNase enzyme into a sample including (i) Cannabis plant matrix derived from lysed cells of a Cannabis plant and (ii) live microbial cells including microbial genomic DNA, (b) introducing an anionic detergent into the sample, where the anionic detergent is in an amount effective to inactivate the DNase enzyme in the sample and lyse the live microbial cells in the sample, (c) introducing a known quantity of control DNA into the sample, (d) contacting the sample with a plurality of polynucleotide capture agents, (e) isolating the plurality of polynucleotide capture agents, thereby isolating polynucleotides from the microbial genomic DNA and/or control DNA in the sample, and (f) amplifying at least a portion of polynucleotides from the microbial genomic DNA isolated in step (e).Type: ApplicationFiled: March 26, 2020Publication date: March 31, 2022Applicant: MEDICINAL GENOMICS CORPORATIONInventors: Kevin J. MCKERNAN, Yvonne HELBERT, Heather EBLING
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Patent number: 11035010Abstract: The present disclosure provides methods and kits for characterizing Cannabis plants. Methods and kits of the present disclosure include detection/amplification of one or more enzymes involved in the production of cannabinoids, such as, for example, tetrahydrocannabinolic acid (THCA) synthase and/or cannabidiolic acid (CBDA) synthase.Type: GrantFiled: December 14, 2018Date of Patent: June 15, 2021Assignee: MEDICINAL GENOMICS CORPORATIONInventor: Kevin J. McKernan
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Patent number: 10358673Abstract: The invention is directed to methods of removing amplicons of non target and/or target nucleic acid sequences having one or more modified (e.g., methylated) nucleotides from a sample wherein the sample comprises the non target nucleic acid and a target nucleic acid sequence to be amplified.Type: GrantFiled: November 22, 2016Date of Patent: July 23, 2019Assignee: Courtagen Life Sciences, Inc.Inventor: Kevin J. McKernan
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Publication number: 20190185946Abstract: The present disclosure provides methods and kits for characterizing Cannabis plants. Methods and kits of the present disclosure include detection/amplification of one or more enzymes involved in the production of cannabinoids, such as, for example, tetrahydrocannabinolic acid (THCA) synthase and/or cannabidiolic acid (CBDA) synthase.Type: ApplicationFiled: December 14, 2018Publication date: June 20, 2019Inventor: Kevin J. McKernan
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Publication number: 20170073732Abstract: The invention is directed to methods of removing amplicons of non target and/or target nucleic acid sequences having one or more modified (e.g., methylated) nucleotides from a sample wherein the sample comprises the non target nucleic acid and a target nucleic acid sequence to be amplified.Type: ApplicationFiled: November 22, 2016Publication date: March 16, 2017Inventor: Kevin J. McKernan
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Patent number: 9512472Abstract: The invention is directed to methods of removing amplicons of non target and/or target nucleic acid sequences having one or more modified (e.g., methylated) nucleotides from a sample wherein the sample comprises the non target nucleic acid and a target nucleic acid sequence to be amplified.Type: GrantFiled: April 14, 2016Date of Patent: December 6, 2016Assignee: Courtagen Life Sciences, Inc.Inventor: Kevin J. McKernan
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Patent number: 9422594Abstract: The invention is directed to methods of removing amplicons of non target and/or target nucleic acid sequences having one or more modified (e.g., methylated) nucleotides from a sample wherein the sample comprises the non target nucleic acid and a target nucleic acid sequence to be amplified.Type: GrantFiled: July 25, 2014Date of Patent: August 23, 2016Assignee: Courtagen Life Sciences, Inc.Inventor: Kevin J. McKernan
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Publication number: 20160230223Abstract: The invention is directed to methods of removing amplicons of non target and/or target nucleic acid sequences having one or more modified (e.g., methylated) nucleotides from a sample wherein the sample comprises the non target nucleic acid and a target nucleic acid sequence to be amplified.Type: ApplicationFiled: April 14, 2016Publication date: August 11, 2016Inventor: Kevin J. McKernan
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Patent number: 9309560Abstract: Methods for producing a paired tag from a nucleic acid sequence are provided in which the paired tag comprises the 5? end tag and 3? end tag of the nucleic acid sequence. In one embodiment, the nucleic acid sequence comprises two restriction endonuclease recognition sites specific for a restriction endonuclease that cleaves the nucleic acid sequence distally to the restriction endonuclease recognition sites. In another embodiment, the nucleic acid sequence further comprises restriction endonuclease recognition sites specific for a rare cutting restriction endonuclease. Methods of using paired tags are also provided. In one embodiment, paired tags are used to characterize a nucleic acid sequence. In a particular embodiment, the nucleic acid sequence is a genome. In one embodiment, the characterization of a nucleic acid sequence is karyotyping. Alternatively, in another embodiment, the characterization of a nucleic acid sequence is mapping of the sequence.Type: GrantFiled: May 19, 2009Date of Patent: April 12, 2016Assignee: Applied Biosystems, LLCInventors: Douglas R. Smith, Joel A. Malek, Kevin J. McKernan
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Publication number: 20140335569Abstract: The invention is directed to methods of removing amplicons of non target and/or target nucleic acid sequences having one or more modified (e.g., methylated) nucleotides from a sample wherein the sample comprises the non target nucleic acid and a target nucleic acid sequence to be amplified.Type: ApplicationFiled: July 25, 2014Publication date: November 13, 2014Inventor: Kevin J. McKernan
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Publication number: 20110257019Abstract: The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.Type: ApplicationFiled: June 29, 2011Publication date: October 20, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kevin J. McKernan, Alan Blanchard, Douglas R. Smith
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Publication number: 20110081687Abstract: The present invention relates to the enrichment of specific target sequences. Enrichment can be achieved through the formation of a heteroduplex that includes the specific target sequence and then the specific cleavage of the heteroduplex. A binding moiety is then added to the cleaved heteroduplex, allowing for the subsequent manipulation of the specific target sequence in the heteroduplex.Type: ApplicationFiled: November 12, 2010Publication date: April 7, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: KEVIN J. MCKERNAN
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Patent number: 7851158Abstract: The present invention relates to the enrichment of specific target sequences. Enrichment can be achieved through the formation of a heteroduplex that includes the specific target sequence and then the specific cleavage of the heteroduplex. A binding moiety is then added to the cleaved heteroduplex, allowing for the subsequent manipulation of the specific target sequence in the heteroduplex.Type: GrantFiled: December 28, 2007Date of Patent: December 14, 2010Assignee: Applied Biosystems, LLCInventor: Kevin J. McKernan
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Publication number: 20100298551Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.Type: ApplicationFiled: December 7, 2009Publication date: November 25, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Douglas R. Smith, Kevin J. McKernan
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Publication number: 20100297628Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.Type: ApplicationFiled: December 7, 2009Publication date: November 25, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Douglas R. Smith, Kevin J. McKernan
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Publication number: 20100028888Abstract: Methods for producing a paired tag from a nucleic acid sequence are provided in which the paired tag comprises the 5? end tag and 3? end tag of the nucleic acid sequence. In one embodiment, the nucleic acid sequence comprises two restriction endonuclease recognition sites specific for a restriction endonuclease that cleaves the nucleic acid sequence distally to the restriction endonuclease recognition sites. In another embodiment, the nucleic acid sequence further comprises restriction endonuclease recognition sites specific for a rare cutting restriction endonuclease. Methods of using paired tags are also provided. In one embodiment, paired tags are used to characterize a nucleic acid sequence. In a particular embodiment, the nucleic acid sequence is a genome. In one embodiment, the characterization of a nucleic acid sequence is karyotyping. Alternatively, in another embodiment, the characterization of a nucleic acid sequence is mapping of the sequence.Type: ApplicationFiled: May 19, 2009Publication date: February 4, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Douglas R. Smith, Joel A. Malek, Kevin J. McKernan
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Patent number: 7645866Abstract: The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.Type: GrantFiled: June 28, 2006Date of Patent: January 12, 2010Assignee: Life Technologies CorporationInventors: Douglas R. Smith, Kevin J. McKernan
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Publication number: 20090280540Abstract: The present invention provides microarrays of oligonucleotide primer pairs, and in particular, microarrays of primers that comprise at least one cleavable linkage. Also provided are methods to capture oligonucleotide primer pairs from one or more microarrays, and methods to use the captured oligonucleotide primer pairs, such as for amplification of a target polynucleotide sequence. In addition, methods of using a microarray to isolate, purify and/or amplify a target polynucleotide are provided.Type: ApplicationFiled: July 16, 2009Publication date: November 12, 2009Applicant: AV ADVANCED GENETIC ANALYISI CORPORATIONInventors: Kevin J. McKernan, Alan Blanchard, Douglas R. Smith
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Publication number: 20090191566Abstract: The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e.g., buffer) that selectively removes (e.g.Type: ApplicationFiled: March 3, 2009Publication date: July 30, 2009Applicant: AGENCOURT BIOSCIENCE CORPORATIONInventors: Kevin J. McKernan, Erik Gustafson, Adrianne D. Brand
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Patent number: 7527929Abstract: The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e.g., buffer) that selectively removes (e.g.Type: GrantFiled: July 29, 2005Date of Patent: May 5, 2009Assignee: Agencourt Bioscience CorporationInventors: Kevin J. McKernan, Erik Gustafson, Adrianne D. Brand