Abstract: Robo1 and Robo2 polypeptides may be produced recombinantly from transformed host cells from the disclosed Robo encoding nucleic acids or purified from human cells. The invention provides isolated Robo hybridization probes and primers capable of specifically hybridizing with the disclosed Robo genes, Robo-specific binding agents such as specific antibodies, and methods of making and using the subject compositions in diagnosis, therapy and in the biopharmaceutical industry.

Abstract: A reusable fitting, connector assembly and kit. The reusable fitting includes a barb portion, a threaded portion, a tightening portion, a connector portion and a substantially smooth cylindrical bore extending through the barb portion, threaded portion, tightening portion and connector portion. The tightening portion is disposed proximate to the threaded portion and includes least two parallel tightening surfaces. The connector assembly includes the fitting, a nut and a crimp sleeve. The kit includes the connector assembly and at least one wrench.

Abstract: The amount of active Robo expressed on a cell is modified by modulating the effective amount of a Comm polypeptide in contact with the cell, whereby the amount of expressed active Robo is modulated inversely with the modulation of the effective amount of the Comm polypeptide in contact with the cell. In a particular embodiment, the Comm polypeptide is provided to the cell by exogenously in a pharmaceutically acceptable composition. In another aspect, the invention provides methods of screening for agents which modulate Robo-Comm interactions. These methods generally involve forming a mixture of a Robo-expressing cell, a Comm polypeptide and a candidate agent, and determining the effect of the agent on the amount of Robo expressed by the cell.