Abstract: A method for identifying and quantifying peptides resulting from enzyme cleavage of collagen type II by mass spectrometric analysis to detect characteristic peptide fragments of a carboxy-terminus having known mass to charge ratios to identify the peptide having that carboxy-terminus. Identification and quantification of the peptides in a biological sample is used to assess activity of proteolytic enzymes in diseases or physiological conditions such as osteoarthritis and rheumatoid arthritis and to assess the efficacy of enzyme blocking agents.

Abstract: A method of determining the concentration of peptides in a biological fluid resulting from the proteolytic degradation of extracellular matrix proteins for diagnosing growth disorders in vertebrates. The method includes determining the concentration of peptides resulting from the proteolytic degradation of extracellular matrix proteins in a biological fluid for determining the efficacy of drugs or agents used to treat growth disorders. A kit for determining the concentration of peptides resulting from the proteolytic degradation of extracellular matrix proteins is also disclosed.

Abstract: A method for identifying and quantifying peptides resulting from enzyme cleavage of collagen type II by mass spectrometric analysis to detect characteristic peptide fragments of a carboxy-terminus having known mass to charge ratios to identify the peptide having that carboxy-terminus. Identification and quantification of the peptides in a biological sample is used to assess activity of proteolytic enzymes in diseases or physiological conditions such as osteoarthritis and rheumatoid arthritis and to assess the efficacy of enzyme blocking agents.

Abstract: A method for identifying and quantifying peptides resulting from enzyme cleavage of collagen type II by mass spectrometric analysis to detect characteristic peptide fragments of a carboxy-terminus having known mass to charge ratios to identify the peptide having that carboxy-terminus. Identification and quantification of the peptides in a biological sample is used to assess activity of proteolytic enzymes in diseases or physiological conditions such as osteoarthritis and rheumatoid arthritis and to assess the efficacy of enzyme blocking agents.

Abstract: This invention is directed to a process for separating a first triglyceride comprising a docosahexaenoic acid residue from a second triglyceride. The process comprises introducing a feed mixture comprising the first triglyceride and the second triglyceride into a chromatographic separation zone, and then isolating a fraction of the feed mixture in the separation zone that has a mass ratio of the first triglyceride to the second triglyceride that is greater than in the feed mixture. The separation zone comprises a stationary phase which comprises metal ions that are capable of coordinating with a double bond of a fatty acid residue of the first triglyceride to form a metal complex with the fatty acid residue.