Abstract: This invention provides a system that allows a user to assemble a chamber apparatus that prohibits samples from leaking or mixing with other samples or chamber array wells, when they are inserted into an array well of the chamber apparatus. In addition, the chamber frame design allows for easy assembly and disassembly for simplified use and slide substrate scanning on conventional microarray scanners. Chamber apparatus includes a chamber frame with an upper integrated gasket and a lower integrated gasket, a substrate, and a substrate frame that positions and captures the substrate. The lower integrated gasket provides a single sealing surface between the chamber frame and the substrate. The upper integrated gasket interfaces with a chamber cover forming a compression seal that prevents sample loss due to evaporation during the hybridization process.

Abstract: A method of fabricating a high vacuum device by providing two major, parallel spaced apart glass sides in a substantial vacuum, forming a continuous edge between the sides and forming a tack with a metallic diffusion bond between the sides. The metallic diffusion bond is formed of materials that bond and cure faster than the material of the continuous edge so that the tack holds the sides in a fixed position while the continuous edge is curing.