Abstract: The primary aspect of the present invention it directed to a three-dimensional matrix, a living tissue equivalent, a tissue microarray, methods of making thereof, and methods of using thereof. In one embodiment of the invention, the three-dimensional matrix comprises fibroblasts and a fibrin matrix. In a preferred embodiment, the three-dimensional matrix comprises blood plasma, thrombin and fibroblasts. The three-dimensional matrix in accordance with this invention may be used to construct living tissue equivalents, including but not limited to skin, blood vessel, bone, tendon, ligaments, and organ equivalents, among many others. Furthermore, the living tissue equivalents may be used to test the effect of various agents and to study the mechanism of disease and the efficacy of various treatment protocols.