Patents by Inventor Kevin Michael Esvelt
Kevin Michael Esvelt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240011002Abstract: The invention relates, in part, to methods of preparing male organisms of a species that include engineering the male organisms in a manner that eliminates post-embryonic viability of the female offspring and female descendants of the engineered male organisms. Certain aspects of the invention include use of such prepared engineered male organisms in methods of controlling population levels of organisms of the species.Type: ApplicationFiled: October 8, 2020Publication date: January 11, 2024Applicant: Massachusetts Institute of TechnologyInventor: Kevin Michael ESVELT
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Publication number: 20230154569Abstract: The invention relates, in part, to methods of accurately and reliably detecting biological sequences corresponding to a particular function.Type: ApplicationFiled: January 23, 2021Publication date: May 18, 2023Applicant: Massachusetts Institute of TechnologyInventors: Kevin Michael Esvelt, Dana Gretton
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Publication number: 20220408702Abstract: The invention relates, in part, to methods to introduce gene alleles of interest into members of a population of a species. The invention also relates to methods to prepare large numbers of organisms comprising one or more introduced alleles of interest in an otherwise wild-type genome.Type: ApplicationFiled: November 20, 2020Publication date: December 29, 2022Applicants: Massachusetts Institute of Technology, Trustees of Tufts CollegeInventors: Kevin Michael Esvelt, Sam Rountree Telford, III
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Publication number: 20220195418Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: ApplicationFiled: December 3, 2021Publication date: June 23, 2022Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Patent number: 11214792Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: GrantFiled: May 13, 2019Date of Patent: January 4, 2022Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Publication number: 20210017516Abstract: The invention relates, in part, to methods and systems with which to identify guide RNAs (gRNAs) and methods and systems with which to prepare, design, and generate gRNAs and minimally repetitive arrays of gRNAs.Type: ApplicationFiled: July 16, 2020Publication date: January 21, 2021Applicant: Massachusetts Institute of TechnologyInventors: Mariah AVILA, Kevin Michael ESVELT, Elizabeth Ashton STRAIT, Emma CHORY
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Publication number: 20200385724Abstract: The invention, in part, includes compositions comprising quadruplet decoding tRNAs and their encoding sequences. The invention also includes assay methods to assess quadruplet decoding as well as methods of preparing quadruplet decoding suppression tRNAs.Type: ApplicationFiled: June 5, 2020Publication date: December 10, 2020Inventors: Ahmed Hussein Badran, Erika Alden DeBenedictis, Kevin Michael Esvelt
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Publication number: 20190276816Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: ApplicationFiled: May 13, 2019Publication date: September 12, 2019Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Publication number: 20190256842Abstract: Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.Type: ApplicationFiled: January 2, 2019Publication date: August 22, 2019Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Jacob Charles Carlson, Ahmed Hussein Badran, Kevin Michael Esvelt
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Patent number: 10336997Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: GrantFiled: June 21, 2016Date of Patent: July 2, 2019Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Patent number: 10179911Abstract: Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.Type: GrantFiled: January 20, 2015Date of Patent: January 15, 2019Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Jacob Charles Carlson, Ahmed Hussein Badran, Kevin Michael Esvelt
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Patent number: 9771574Abstract: Aspects of the disclosure relate to an apparatus for continuous directed evolution of a gene of interest. In some embodiments, the apparatus comprises a cellstat and a turbidostat. In some embodiments, the apparatus is useful in methods of passing a nucleic acid from cell to cell in a desired function-dependent manner.Type: GrantFiled: May 5, 2015Date of Patent: September 26, 2017Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt
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Publication number: 20170009224Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: ApplicationFiled: June 21, 2016Publication date: January 12, 2017Applicant: President and Fellows of HarvardInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Publication number: 20160348096Abstract: Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.Type: ApplicationFiled: January 20, 2015Publication date: December 1, 2016Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Jacob Charles Carlson, Ahmed Hussein Badran, Kevin Michael Esvelt
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Patent number: 9394537Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: GrantFiled: December 22, 2011Date of Patent: July 19, 2016Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Publication number: 20150275202Abstract: The present invention discloses generalizable methods of evolving nucleic acids and proteins utilizing continuous directed evolution. The invention discloses methods of passing a nucleic acid from cell to cell in a desired function-dependent manner. The linkage of the desired function and passage of the nucleic acid from cell to cell allows for continuous selection and mutation of the nucleic acid.Type: ApplicationFiled: May 5, 2015Publication date: October 1, 2015Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt
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Patent number: 8734823Abstract: Devices, methods, and systems are described for administration to at least one biological tissue of at least one device including at least one altered microorganism. In an embodiment, the altered microorganism includes at least one nucleic acid construct encoding at least one therapeutic agent.Type: GrantFiled: May 28, 2010Date of Patent: May 27, 2014Assignee: The Invention Science Fund I, LLCInventors: Dario G. Amodei, Mahalaxmi Gita Bangera, Xiaoyan Robert Bao, Anna Bershteyn, Brett Bethke, Philip A. Eckhoff, Kevin Michael Esvelt, Kyle B. Gustafson, Edward K. Y. Jung, William Michael Kaminsky, Jordin T. Kare, Lily Yvonne Kim, Eric C. Leuthardt, Erez Lieberman, Ankur Moitra, Christopher Somogyi, Clarence T. Tegreene, Lowell L. Wood, Jr., Jeremiah James Zartman
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Patent number: 8682619Abstract: Devices, methods, and systems are described for administration to at least one biological tissue of at least one device including at least one altered microorganism. In an embodiment, the altered microorganism includes at least one nucleic acid construct encoding at least one therapeutic agent.Type: GrantFiled: May 28, 2010Date of Patent: March 25, 2014Assignee: The Invention Science Fund I, LLCInventors: Dario G. Amodei, Mahalaxmi Gita Bangera, Xiaoyan Robert Bao, Anna Bershteyn, Brett Bethke, Philip A. Eckhoff, Kevin Michael Esvelt, Kyle B. Gustafson, Edward K. Y. Jung, William Michael Kaminsky, Jordin T. Kare, Lily Yvonne Kim, Eric C. Leuthardt, Erez Lieberman, Ankur Moitra, Christopher Somogyi, Clarence T. Tegreene, Lowell L. Wood, Jr., Jeremiah James Zartman
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Publication number: 20130345064Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.Type: ApplicationFiled: December 22, 2011Publication date: December 26, 2013Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
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Publication number: 20110172826Abstract: Devices, methods, and systems are described for administration to at least one biological tissue of at least one device including at least one altered microorganism. In an embodiment, the altered microorganism includes at least one nucleic acid construct encoding at least one therapeutic agent.Type: ApplicationFiled: May 28, 2010Publication date: July 14, 2011Inventors: Dario G. Amodei, Mahalaxmi Gita Bangera, Xiaoyan Robert Bao, Anna Bershteyn, Brett Bethke, Philip A. Eckhoff, Kevin Michael Esvelt, Kyle Gustafson, Edward K.Y. Jung, William Michael Kaminsky, Jordin T. Kare, Lily Yvonne Kim, Eric C. Leuthardt, Erez Lieberman, Ankur Moitra, Christopher Somogyi, Clarence T. Tegreene, Lowell L. Wood, JR., Jeremiah James Zartman