Abstract: Cell lines, such as iPSC cell lines, or other tissue constructs created from tissue samples collected from large numbers of donors, are used in assessing the biological reactions of individuals to biological or chemical agents. The donor tissue samples are grouped according to a phenotypic or genotypic trait that is exogenous to the results of the assays conducted. Cells or tissues derived from the samples of each of the individuals are combined with one or more chemical or biological agents and tested in identical assays to determine the impact of the chemical or biological agents. The results of each separately defined phenotypic or genotypic subgroup are aggregated to form a distribution of impacts for that particular group. The distributions associated with the various groups are compared to determine whether there is a statistically meaningful difference in the responses of the groups.

Abstract: Methods are provided for establishing a threshold exposure limit to a chemical or biological agent for the members of a target species taking into account the potential for variation in responses among the members of that species. Exemplary exposures include maximum safe exposure to a toxic agent, or minimum effective dose for a pharmaceutical. The method is particularly useful under circumstances wherein variation in responsiveness to the agent cannot be ascertained by directly exposing members of the target species to the agent.

Abstract: A method is provided for estimating or predicting, a priori, the incidence or degree of biological reaction by an individual in a population of interest when exposed to a chemical or biological agent of interest in vivo. The method uses a combination of data from in vitro experiments using the agent of interest, from in vitro experiments using a reference agent, and data obtained regarding the incidence or degree of the in vivo effects of the reference agent.

Abstract: Methods are provided herein for conducting stimulus-response studies on iPSCs, or cells derived from iPSCs, that have been derived from perinatal cells collected from donors under null-exposome conditions. In some embodiments, multiple donors are involved. In other embodiments, the iPSCs are differentiated. The use of induced pluripotent stem cells (iPSCs) derived from cells that originate from neonates enable a scientist to largely remove the influences of age and environmental exposures, allowing a more targeted analysis of the direct interaction between a stimulus and a test subject. Furthermore, use of iPSCs derived from cells originating from multiple donors enable the scientist to obtain more precise measurements of the role of genetic differences in determining responses to a given stimulus than the use of other test materials, by eliminating the vast majority of the differential influences of age and environment among test subjects.

Abstract: Platforms and methods for conducting stimulus-response studies on induced pluripotent stem cells, or cells differentiated therefrom, that have been derived from cells collected from donors. Samples are taken at various ages and/or before and after exposure to environmental conditions. Methods for preparing the platforms are also provided. In one embodiment, multiple donors are involved. In another embodiment, induced pluripotent stem cells are differentiated. Optionally, the induced pluripotent stem cells or differentiated cells thereof, are preserved for subsequent analysis.

Abstract: Methods are provided for improving the impact of genetically diversified stimulus response gene association (GDSRGA) studies. The methods may involve developing subpopulations to be contrasted in GDSRGA studies by obtaining a biological sample from each donor of a population of donors; selecting a common cohort from the biological samples by obtaining at least a partial genomic sequence from each biological sample, aligning the sequences of the biological samples, and removing biological samples that cannot be sequenced accurately or fail to align; applying a test molecule or condition to the biological samples to induce phenotypically distinct responses among the members of the cohort; and segregating the biological samples into subpopulations based on the phenotypically distinct responses. These subpopulations may be used in GDSRGA studies.

Abstract: Methods for predicting differential human responses to chemical and biological substances using extra-embryonic pluripotent or multipotent stem cells from at least 20 different donors. In one embodiment, the extra-embryonic pluripotent or multi-potent stem cells are differentiated. The method is useful for predicting and elucidating differential human responses to chemical and biological substances in vitro across a genetically diverse population.

Abstract: The invention comprises systems and methods for making a prediction utilizing admissions-based or personal information. The invention receives information associated with the prospective student or person via a network. The invention determines one or more predictive factors based upon selected prospective student information or selected personal information. Finally, the invention determines a likelihood of a decision such as an enrollment decision based upon at least one predictive factor. Information utilized by the invention consists of at least one of the following: static data, biographical data, statistical data, historical data, behavioral data, preferential data, circumstantial data, demographic data, or other data that permits an observation to be made about a person such as the prospective student. The invention develops and updates a predictive algorithm that correlates one or more predictive factors based upon selected prospective student information or personal information.