Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.

Abstract: Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.

Abstract: Fusion transcripts are provided herein. In exemplary embodiments, the fusion transcript is encoded by a nucleic acid molecule comprising a general structure A-B, wherein structure A is a portion of a gene listed in Column A of Table 1 and structure B is a portion of a gene listed in Column B of Table 1, wherein the gene listed in Column A and the gene listed in Column B are listed in the same row of Table 1, wherein structure B is located immediately 3? to structure A. Polypeptides encoded by the fusion transcript, nucleic acid molecules encoding the fusion transcript, and nucleic acid molecules comprising the reverse complement sequence of the fusion transcript, are additionally provided. Related expression vectors, host cells, binding agents, kits, and methods of using the same are further provided herein.

Abstract: Architecture for operating an industrial solution on top of a performance management platform using a custom business type library. The platform can plug/unplug the custom business type library, interacts with the type library to ensure data integrity and enforce business rules, and simplifies localizations of the industrial solution. The custom type library extends the capability to define specific behavior for models and dimensions, and create and define content specific to any industry, reuse, and packaging. The custom type library inherits from a generic type library of the platform. The customized type library defines business object types and associates business rules therewith. Through a common interface, the performance management platform can iterate through the business rule chains and enforce/apply the business rules during the operations.

Abstract: Spray reducing apparatus (14) for a vehicle comprises a duct (16) adapted to be disposed in front of a vehicle wheel (10) and arranged to collect air in a forwardly directed mouth (18). The air is directed through an exit (20) towards the ground in front of the wheel so as to deflect any surface water away from the path of the wheel.One of such apparatus is arranged in front of each front wheel of the vehicle with the exit of the duct being disposed in the path of the vehicle wheel below the axle line thereof.