Abstract: This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.

Abstract: This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.

Abstract: This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.

Abstract: This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.

Abstract: This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.

Abstract: This invention relates to a method and apparatus for detecting a biological molecule associated with enzyme activity in a sample. The invention is applicable to detecting a microorganism associated with an enzyme in a sample such as water, food, soil, or a biological sample. According to a preferred embodiment of the method of the invention, a sample containing an enzyme of interest or a microorganism associated with the enzyme is combined with a suitable substrate, and a fluorescent product of the enzyme-substrate reaction is selectively detected. The fluorescent product is detected with a partitioning element or optical probe/partitioning element of the invention. In one embodiment the partitioning element provides for partitioning of only the fluorescent product molecule into the probe. The invention also provides an automated system for monitoring for biological contamination of water or other samples.

Abstract: A mining machine that has a chassis that defines an operator area thereon and has a mining assembly attached thereto. A vertically extending shield assembly is supported on the chassis to prevent material that is dislodged during the mining process from inadvertently entering the operator area. The shield assembly may be selectively vertically extendable and may be equipped with a ventilation duct assembly to withdraw dust and gases away from the operator area. The mining machine may be equipped with a mining assembly that extends the entire width of the chassis and that can be advanced forwardly of the chassis and swung down across the seam face during mining. The present invention may also be equipped with a quick disconnect assembly for detachably mounting the mining assembly or other mining implement to the mining machine.

Abstract: Disclosed is a conveyor belt/cutting head advancement mechanism (10). Mechanism (10) comprises a conveyor belt support structure (11) connected at one end to a conveyor belt gathering structure (12). The conveyor belt support structure (11) is typically interconnected at its forward end to a self advancing conveyor return end (not shown). Material removed from a mine face by the cutting implement is delivered to a conveyor belt supported by the support structure (11). Structure (11) comprises a plurality of structural elements (18) pivotally interconnected in a scissor-like configuration. The mid points of elements (18) are pivotally interconnected at a point coinciding with the mounting location of a conveyor belt return roller (16). At the upper pivotally interconnected extremities of elements (18), the conveyor belt advancing rollers are supported. Mounted at a location nearby the lower ends of elements (18) are ground engaging wheels (17).

Abstract: Disclosed is an extendible conveyor structure (10). Structure (10) comprises a main frame (11) supported above a ground surface by way of wheels (20). Main frame (11) comprises a number of vertical members (22) and cross-members (18) extending therebetween. Main frame (11) further comprises a number of longitudinal members (23). Either fixed to main frame (11) or rollably supported thereby is a sub-frame (14) to support a conveyor belt (16). Rollably supported to sub-frame (14) is a pair of movable sub-frames (12, 13). The conveyor belt (16) spans all three sub-frames (12, 13 and 14) and is supported by rollers (15) or alternatively by troughing plates or a combination of both. The respective sub-frames (12, 13 and 14) may be manually or automatically extended between a loading zone and a discharge zone as required.

Abstract: Disclosed is a system for dispensing and supplementing a large volume of liquid for use with cell and tissue cultures. The system disclosed comprises a large volume drum having liquid contained therein. The system disclosed further comprises a first assembly for dispensing the liquid from the large volume drum. The system disclosed further comprises a second assembly for supplementing the first liquid contained in the large volume drum with the second liquid. The system disclosed may also comprise a third mechanical arrangement for mixing the first and second liquids.

Abstract: Disclosed is a system for dispensing and supplementing a large volume of liquid for use with cell and tissue cultures. The system disclosed comprises a large volume drum having liquid contained therein. The system disclosed further comprises a first assembly for dispensing the liquid from the large volume drum. The system disclosed further comprises a second assembly for supplementing the first liquid contained in the large volume drum with the second liquid. The system disclosed may also comprise a third mechanical arrangement for mixing the first and second liquids. The materials chosen are compatible with gamma irradiation sterilization process, sterile cell culture media, and standard distribution systems.