Patents by Inventor Kevin Roy

Kevin Roy has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Multiplex production and barcoding of genetically engineered cells
    Patent number: 12416015
    Abstract: The present disclosure relates to multiplex production and phenotyping of genetically engineered cells using RNA-guided nucleases and genomic barcoding. In particular, high-throughput multiplex genome editing is achieved utilizing a system that facilitates precise genome editing at desired target chromosomal loci by homology directed repair. Integration of guide RNA and donor DNA sequences as a genomic barcode at a separate chromosomal locus allows identification, isolation, and massively-parallel validation of individual variants from a pool of transformants. Strains can be arrayed according to their precise genetic modifications, as specified by donor DNA incorporation in heterologous or native genes.
    Type: Grant
    Filed: September 14, 2018
    Date of Patent: September 16, 2025
    Assignees: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY, BRANDEIS UNIVERSITY
    Inventors: Kevin Roy, Justin D. Smith, Robert P. St. Onge, Lars M. Steinmetz, James E. Haber
  • PRODUCTION AND TRACKING OF ENGINEERED CELLS WITH COMBINATORIAL GENETIC MODIFICATIONS
    Publication number: 20220389415
    Abstract: Described herein are methods for making genetically modified cells by introducing combinations of genetic variants (designed or random) or constructs (genes or otherwise arbitrary DNA) into a population of cells, and for tracking each variant combination by sequentially building an array of barcodes at a common locus (chromosomal or plasmid), termed the barcode locus. Also described are the cells made by such methods.
    Type: Application
    Filed: February 7, 2020
    Publication date: December 8, 2022
    Inventors: Marc SALIT, Lars M. STEINMETZ, Robert St. ONGE, Justin D. SMITH, Kevin ROY
  • MULTIPLEX PRODUCTION AND BARCODING OF GENETICALLY ENGINEERED CELLS
    Publication number: 20200270632
    Abstract: The present disclosure relates to multiplex production and phenotyping of genetically engineered cells using RNA-guided nucleases and genomic barcoding. In particular, high-throughput multiplex genome editing is achieved utilizing a system that facilitates precise genome editing at desired target chromosomal loci by homology directed repair. Integration of guide RNA and donor DNA sequences as a genomic barcode at a separate chromosomal locus allows identification, isolation, and massively-parallel validation of individual variants from a pool of transformants. Strains can be arrayed according to their precise genetic modifications, as specified by donor DNA incorporation in heterologous or native genes.
    Type: Application
    Filed: September 14, 2018
    Publication date: August 27, 2020
    Inventors: Kevin Roy, Justin D. Smith, Robert P. St. Onge, Lars M. Steinmetz, James E. Haber
  • Process for the manufacture of acrolein
    Patent number: 9115067
    Abstract: The invention generally relates to the manufacture of acrolein. In particular, the invention generally relates to a process for the continuous production of acrolein via the catalytic oxidation of propylene.
    Type: Grant
    Filed: December 22, 2014
    Date of Patent: August 25, 2015
    Assignee: Novus International, Inc.
    Inventors: Donald L. Bunning, James D. Rolston, David A. LaBrot, Puvin Pichai, John Patrick Dever, Olan Stanley Fruchey, Michael L. Hutchison, Kevin Roy