Abstract: This invention relates to transduction of cargo molecules into living cells, such as protein transduction, in particular a delivery molecule for transduction of a cargo into a cell comprising: a cargo-binding molecule and/or a cargo; a glycosaminoglycan (GAG) binding element, which is capable of binding to GAG on the surface of the cell; and a protein transduction domain. Methods of transduction, methods of producing or modifying cargo for transduction, delivery molecules for transduction and methods of treatment using transduction, or using transduced cells are also provided.

Abstract: This invention relates to transduction of cargo molecules into living cells, such as protein transduction, in particular a delivery molecule for transduction of a cargo into a cell comprising: a cargo-binding molecule and/or a cargo; a glycosaminoglycan (GAG) binding element, which is capable of binding to GAG on the surface of the cell; and a protein transduction domain. Methods of transduction, methods of producing or modifying cargo for transduction, delivery molecules for transduction and methods of treatment using transduction, or using transduced cells are also provided.

Abstract: This invention relates to transduction of cargo molecules into living cells, such as protein transduction, in particular a delivery molecule for transduction of a cargo into a cell comprising: a cargo-binding molecule and/or a cargo; a glycosaminoglycan (GAG) binding element, which is capable of binding to GAG on the surface of the cell; and a protein transduction domain. Methods of transduction, methods of producing or modifying cargo for transduction, delivery molecules for transduction and methods of treatment using transduction, or using transduced cells are also provided.

Abstract: This invention relates to transduction of cargo molecules into living cells, such as protein transduction, in particular a delivery molecule for transduction of a cargo into a cell comprising: a cargo-binding molecule and/or a cargo; a glycosaminoglycan (GAG) binding element, which is capable of binding to GAG on the surface of the cell; and a protein transduction domain. Methods of transduction, methods of producing or modifying cargo for transduction, delivery molecules for transduction and methods of treatment using transduction, or using transduced cells are also provided.

Abstract: The invention provides polymer particles that are obtainable by a method selected from emulsion methods, diffusion methods and evaporation methods carried out in the presence of surface-engineering surfactant which is one or more polymer that displays a lower critical solution temperature, in aqueous media, that is between 10 to 90° C., this polymer being the polymerization product of one or more monomer selected from polymerisable alkyleneglycol acrylate monomers and polymerisable alkyleneglycol methacrylate monomers. The polymer particles can be used in controlled release applications, such as flavour release applications, fragrance release applications and biomedical applications. The invention also provides a cell support matrix comprising the polymer particles.

Abstract: The invention provides polymer particles that are obtainable by a method selected from emulsion methods, diffusion methods and evaporation methods carried out in the presence of surface-engineering surfactant which is one or more polymer that displays a lower critical solution temperature, in aqueous media, that is between 10 to 90° C., this polymer being the polymerisation product of one or more monomer selected from polymerisable alkyleneglycol acrylate monomers and polymerisable alkyleneglycol methacrylate monomers. The polymer particles can be used in controlled release applications, such as flavour release applications, fragrance release applications and biomedical applications. The invention also provides a cell support matrix comprising the polymer particles.

Abstract: The invention generally provides compositions and methods that promote wound healing. Such compositions comprise isolated L. sericata polypeptides having serine protease activity. Desirably, the serine protease degrades fibronectin. The invention further provides biologically active fragments of fibronectin that promote wound healing that are the degradation products of incubation with ES.

Abstract: Process for preparing active polymer extrudate comprising polymer matrix and guest matter, the process comprising contacting a polymer substrate and guest matter with a plasticising fluid under dense phase, sub critical or supercritical plasticising conditions of elevated temperature and/or pressure to plasticise the polymer substrate and incorporate guest matter and extruding polymer substrate incorporating guest matter under dense phase, sub critical or supercritical conditions via an extrusion orifice into a collection zone or a mould with simultaneous or subsequent release of pressure, whereby extrudate is obtained comprising a solid admixture of polymer matrix and guest matter in form conferred by the orifice or the mould; a novel extrudate; composition thereof and apparatus for the preparation thereof, and use thereof in fibre processing techniques, medical applications such as in delivery of drugs and other agents such as imaging and diagnostic agents, tissue engineering, and as medical devices or aids

Abstract: A process for the preparation of a polymer composite loaded with functioning matter wherein the process comprises contacting a polymer substrate and functioning matter with a plasticising fluid or mixture of plasticising fluids under plasticising conditions to plasticise and/or swell the polymer and incorporate the functioning matter, and releasing the plasticising fluid to obtain the polymer composite, wherein contacting is at a pressure in the range 1 to 1000 bar and a temperature in the range ?200 to +500C, selected in manner that at least a proportion of functioning matter does not freeze or refreeze during processing, or if at a temperature at which freezing or refreezing may occur, that either matter is desiccated or a pressure constraint is applied whereby pressure is in a range having a maximum pressure less than 1000 bar throughout contact of functioning matter and plasticising fluid, whereby at least a proportion of functioning matter retains its function in the polymer composite; A polymer composit

Abstract: A process for the preparation of a polymer composite comprising internally distributed deposition matter wherein the process comprises providing a deposit of deposition matter at the surface of a solid state polymer substrate, contacting the surface deposited polymer with a plasticising fluid or a mixture of plasticising fluids under plasticising conditions to plasticise and/or swell the polymer and internally distribute deposition matter, and releasing the plasticising fluid or fluids to obtain polymer composite.; A polymer composite comprising a porous or non porous polymer throughout which particulate deposition matter as hereinbefore defined is distributed with desired uniformity, preferably with high uniformity in excess of 80% for example in excess of 98%.

Abstract: The invention provides a biodegradable and biocompatible polymer article having a surface wherein a biologically active ligand is provided on said surface in a spatially controlled pattern. The pattern may be formed using a poly(dimethyl siloxane) mold. The biologically active ligand may be attached to the polymer article by a biotin-avidin-biotin linkage.

Abstract: The present invention provides a novel surface engineering strategy that uses biomolecular interactions to immobilize surface modifying ligands on biomaterial architectures. The surface modified compositions resulting from the inventive method are useful in many contexts, including, but not limited to, scaffolds for tissue engineering and as vehicles for site specific drug delivery. In one preferred embodiment, the biomolecular interaction is achieved by using an “anchor-adapter-tag” system, in which an adapter which can interact specifically and with high selectivity with an anchor molecule (present on the biodegradable surface) and a tag (bound to the ligand to be immobilized) simultaneously is used in attaching the ligand to the surface in a manner which is stable in vitro or in vivo.