Abstract: Provided herein are compositions and methods for detecting exhaled microRNAs for identifying lung diseases such as cancer, asthma, and chronic obstructive pulmonary disease.

Abstract: A method for sensing a leak in a unit under test includes scanning a SKU on a unit under test, sending the SKU information to a compute, connecting the unit under test to a testing apparatus, removing gas from an interior volume of the unit under test, monitoring the vacuum pressure within the interior volume of the unit under test until the vacuum pressure reaches a test pressure, initiating delivery of helium to the exterior of the unit under test through a helium gun by pulling a trigger on the helium gun to dispense helium therefrom, positioning the helium supply gun so that helium is released over a weld of the unit under test, detecting the movement speed of the helium supply gun during the dispensing of helium therefrom, and displaying the helium content internal to the unit under test on a graphical user interface.

Abstract: A method for sensing a leak in a unit under test includes scanning a SKU on a unit under test, sending the SKU information to a compute, connecting the unit under test to a testing apparatus, removing gas from an interior volume of the unit under test, monitoring the vacuum pressure within the interior volume of the unit under test until the vacuum pressure reaches a test pressure, initiating delivery of helium to the exterior of the unit under test through a helium gun by pulling a trigger on the helium gun to dispense helium therefrom, positioning the helium supply gun so that helium is released over a weld of the unit under test, detecting the movement speed of the helium supply gun during the dispensing of helium therefrom, and displaying the helium content internal to the unit under test on a graphical user interface.

Abstract: A method for sensing a leak in a unit under test includes scanning a SKU on a unit under test, sending the SKU information to a compute, connecting the unit under test to a testing apparatus, removing gas from an interior volume of the unit under test, monitoring the vacuum pressure within the interior volume of the unit under test until the vacuum pressure reaches a test pressure, initiating delivery of helium to the exterior of the unit under test through a helium gun by pulling a trigger on the helium gun to dispense helium therefrom, positioning the helium supply gun so that helium is released over a weld of the unit under test, detecting the movement speed of the helium supply gun during the dispensing of helium therefrom, and displaying the helium content internal to the unit under test on a graphical user interface.

Abstract: The present disclosure provides coupling methods and systems for producing peptides or proteins using semi-continuous or continuous manufacturing techniques to enable rapid production of peptides and proteins using solid phase peptide synthesis (SPPS). The disclosure provides a higher degree of process control for peptide and protein product manufacturing using semi-continuous or continuous manufacturing techniques with inline analytics and automation.

Abstract: The present disclosure provides computer-based methods and systems for controlling peptide synthesis. An embodiment begins by providing a manufacturing process that synthesizes peptides using solid phase slug flow. In turn, the manufacturing process is automated through use of a machine learning engine by selecting values for operating conditions for the manufacturing process. In such an embodiment, a given operating condition is flow rate profile. An embodiment generates an indication of the selected values for the operating conditions and controls the manufacturing process therewith.

Abstract: A method of selectively etching a semiconductor device and manufacturing a BSI image sensor device includes etching a doped silicon substrate with an HNA solution for a predetermined time duration to obtain an etching solution having a concentration C1 of nitrite ions, etching the semiconductor device using the obtained etching solution. Etching the semiconductor device requires an initial concentration C0 of nitride ions that is lower than C1. The HNA solution comprises a hydrofluoric acid (HF), a nitric acid (HNO3), and a acetic acid (CH3COOH). The BSI image sensor device will have a uniform thickness when etched using the thus obtained etching solution.

Abstract: A method of selectively etching a semiconductor device and manufacturing a BSI image sensor device includes etching a doped silicon substrate with an HNA solution for a predetermined time duration to obtain an etching solution having a concentration C1 of nitrite ions, etching the semiconductor device using the obtained etching solution. Etching the semiconductor device requires an initial concentration C0 of nitride ions that is lower than C1. The HNA solution comprises a hydrofluoric acid (HF), a nitric acid (HNO3), and a acetic acid (CH3COOH). The BSI image sensor device will have a uniform thickness when etched using the thus obtained etching solution.

Abstract: Methods and kits are provided for testing the functional effect of methylating different cytosine residues, for testing patterns of DNA methylation on gene expression, and for site-specific methylation, as well as methylated DNA constructs. Methods are provided that include steps of denaturing a circular double-stranded DNA construct; hybridizing primers to separate strands of the denatured circular DNA construct; contacting the hybridized primers with a DNA polymerase, deoxynucleoside triphosphates, and copies of the primers; contacting nicked copies with a DNA ligase so as to form a copy of the circular DNA construct; contacting the copy of the circular DNA construct with a methyltransferase enzyme; transfecting a cell with C-5 methylated circular DNA construct; and quantifying expression of a gene of interest, thereby determining the effect of C-5 methylating cytosine nucleotide residues of DNA on expression of the gene of interest.

Abstract: Methods and kits are provided for testing the functional effect of methylating different cytosine residues or testing patterns of DNA methylation on gene expression. Methods are also provided for site-specific methylation, as well as methylated DNA constructs.