Abstract: Kits and methods of using the kits for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding are disclosed. The sample analysis kits employ nucleic acid encoding and/or nucleic acid recording of a molecular interaction and/or reaction, such as recognition events (e.g., between an antigen and an antibody, between a modified terminal amino acid residue, or between a small molecule or peptide therapeutic and a target, etc.). Additional barcoding reagents, such as those for cycle-specific barcoding (e.g., “clocking”), compartment barcoding, combinatorial barcoding, spatial barcoding, or any combination thereof, may be included in the kits. The sample may comprise macromolecules, including peptides, polypeptides, and proteins, and the recording may generate molecular interaction and/or reaction information, and/or polypeptide sequence information.

Abstract: The present disclosure relates to methods and intermediates useful for preparing a compound of formula I: or a co-crystal, solvate, salt or combination thereof.

Abstract: Provided herein are compositions and methods for detecting exhaled microRNAs for identifying lung diseases such as cancer, asthma, and chronic obstructive pulmonary disease.

Abstract: Kits and methods of using the kits for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding are disclosed. The sample analysis kits employ nucleic acid encoding and/or nucleic acid recording of a molecular interaction and/or reaction, such as recognition events (e.g., between an antigen and an antibody, between a modified terminal amino acid residue, or between a small molecule or peptide therapeutic and a target, etc.). Additional barcoding reagents, such as those for cycle-specific barcoding (e.g., “clocking”), compartment barcoding, combinatorial barcoding, spatial barcoding, or any combination thereof, may be included in the kits. The sample may comprise macromolecules, including peptides, polypeptides, and proteins, and the recording may generate molecular interaction and/or reaction information, and/or polypeptide sequence information.

Abstract: Crystalline forms of Compound I: pharmaceutically acceptable salts thereof, and solvates and hydrates thereof are disclosed. Pharmaceutical compositions comprising the same, methods of treating cystic fibrosis using the same, and methods for making the same are also disclosed.

Abstract: An online concierge system receives information describing the progress of a picker servicing a batch of existing orders and predicts a first likelihood the picker will finish servicing the batch within a threshold amount of time based on the picker's progress and information describing the batch. If the first likelihood exceeds a threshold likelihood, the system accesses a machine learning model trained to predict a second likelihood the picker will accept a batch of new orders for servicing while servicing the batch of existing orders. The system applies the model to inputs including a set of attributes of the picker and the picker's progress to predict the second likelihood. The system matches batches of new orders with pickers based on the second likelihood and sends one or more requests to service one or more batches matched with the picker to a client device associated with the picker.

Abstract: Methods and Kits for analyzing macromolecules, including peptides, polypeptides, and proteins, employing nucleic acid encoding are disclosed. The sample analysis kits employ nucleic acid encoding and/or nucleic acid recording of a molecular interaction and/or reaction, such as recognition events (e.g., between an antigen and an antibody, between a modified terminal amino acid residue, or between a small molecule or peptide therapeutic and a target, etc.). Assays that do not require the cyclic transfer of information between a coding tag and a recording tag are also disclosed, including single cycle assays.

Abstract: A system for digital control of an analog pedal has an analog audio circuit. The analog audio circuit receives an analog audio signal as an input audio signal and processes the input audio signal into an output analog audio signal. A digital control system is operatively coupled to the analog audio circuit. The digital control system receives a user input and outputs a control signal to the analog audio circuit as a function of the user input. The analog audio circuit modifies the audio signal into the output analog audio signal as a function of the control signal.

Abstract: The disclosure provides at least one entity chosen from compounds of formula (I), solid state forms of the same, compositions comprising the same, and methods of using the same, including use in treating focal segmental glomerulosclerosis (FSGS) and/or non-diabetic kidney disease (NDKD).

Abstract: An online concierge system receives information describing the progress of a picker servicing a batch of existing orders and a service request for an order. The system identifies picker attributes of the picker and order attributes of the order and each existing order of the set and accesses a machine learning model trained to predict a likelihood the picker will accept an add-on request to add the order to the batch of existing orders. To predict the likelihood, the system applies the model to the picker attributes, the progress of the picker, and the order attributes. The system determines a cost associated with sending the add-on request to the picker based on the likelihood and assigns the order to a set of orders based on the cost. The system sends the add-on request to the picker responsive to determining the order is assigned to the batch of existing orders.

Abstract: A camera system for a venue including a camera mounted above the venue; a server including artificial intelligence (AI) adapted to follow a subject having a position; the AI adapted to generate aiming instructions based on the position of the subject. The camera being in communication with the server to receive and implement the aiming instructions. The camera system may include a wide area LED light mounted to a light pole. The camera may be mounted to the light pole. The camera system may include wide area LED light is in communication with the server. The wide area LED light produces light at an intensity and the server is adapted to vary the intensity based on the position of the subject.

Abstract: In some examples, an endovascular medical device system includes an elongated body configured to be introduced in a blood vessel of a patient. The system includes an expandable structure at a distal portion of the elongated body. The expandable structure includes an expandable body portion including a plurality of interconnected struts. The expandable structure further includes a plurality of electrode attachment elements, where each electrode attachment element of the plurality of electrode attachment elements includes a first projection and a second projection branching off of at least one strut of the plurality of interconnected struts. In some examples, the system includes one or more electrodes coupled to the expandable structure via the plurality of electrode attachment elements. In some examples, each electrode of the one or more electrodes is configured to receive the first projection and the second projection to couple the respective electrode to the expandable structure.

Abstract: A method for sensing a leak in a unit under test includes scanning a SKU on a unit under test, sending the SKU information to a compute, connecting the unit under test to a testing apparatus, removing gas from an interior volume of the unit under test, monitoring the vacuum pressure within the interior volume of the unit under test until the vacuum pressure reaches a test pressure, initiating delivery of helium to the exterior of the unit under test through a helium gun by pulling a trigger on the helium gun to dispense helium therefrom, positioning the helium supply gun so that helium is released over a weld of the unit under test, detecting the movement speed of the helium supply gun during the dispensing of helium therefrom, and displaying the helium content internal to the unit under test on a graphical user interface.

Abstract: A method for sensing a leak in a unit under test includes scanning a SKU on a unit under test, sending the SKU information to a compute, connecting the unit under test to a testing apparatus, removing gas from an interior volume of the unit under test, monitoring the vacuum pressure within the interior volume of the unit under test until the vacuum pressure reaches a test pressure, initiating delivery of helium to the exterior of the unit under test through a helium gun by pulling a trigger on the helium gun to dispense helium therefrom, positioning the helium supply gun so that helium is released over a weld of the unit under test, detecting the movement speed of the helium supply gun during the dispensing of helium therefrom, and displaying the helium content internal to the unit under test on a graphical user interface.

Abstract: A method for sensing a leak in a unit under test includes scanning a SKU on a unit under test, sending the SKU information to a compute, connecting the unit under test to a testing apparatus, removing gas from an interior volume of the unit under test, monitoring the vacuum pressure within the interior volume of the unit under test until the vacuum pressure reaches a test pressure, initiating delivery of helium to the exterior of the unit under test through a helium gun by pulling a trigger on the helium gun to dispense helium therefrom, positioning the helium supply gun so that helium is released over a weld of the unit under test, detecting the movement speed of the helium supply gun during the dispensing of helium therefrom, and displaying the helium content internal to the unit under test on a graphical user interface.

Abstract: The present disclosure provides coupling methods and systems for producing peptides or proteins using semi-continuous or continuous manufacturing techniques to enable rapid production of peptides and proteins using solid phase peptide synthesis (SPPS). The disclosure provides a higher degree of process control for peptide and protein product manufacturing using semi-continuous or continuous manufacturing techniques with inline analytics and automation.

Abstract: The present disclosure provides computer-based methods and systems for controlling peptide synthesis. An embodiment begins by providing a manufacturing process that synthesizes peptides using solid phase slug flow. In turn, the manufacturing process is automated through use of a machine learning engine by selecting values for operating conditions for the manufacturing process. In such an embodiment, a given operating condition is flow rate profile. An embodiment generates an indication of the selected values for the operating conditions and controls the manufacturing process therewith.

Abstract: A method of selectively etching a semiconductor device and manufacturing a BSI image sensor device includes etching a doped silicon substrate with an HNA solution for a predetermined time duration to obtain an etching solution having a concentration C1 of nitrite ions, etching the semiconductor device using the obtained etching solution. Etching the semiconductor device requires an initial concentration C0 of nitride ions that is lower than C1. The HNA solution comprises a hydrofluoric acid (HF), a nitric acid (HNO3), and a acetic acid (CH3COOH). The BSI image sensor device will have a uniform thickness when etched using the thus obtained etching solution.

Abstract: A method of selectively etching a semiconductor device and manufacturing a BSI image sensor device includes etching a doped silicon substrate with an HNA solution for a predetermined time duration to obtain an etching solution having a concentration C1 of nitrite ions, etching the semiconductor device using the obtained etching solution. Etching the semiconductor device requires an initial concentration C0 of nitride ions that is lower than C1. The HNA solution comprises a hydrofluoric acid (HF), a nitric acid (HNO3), and a acetic acid (CH3COOH). The BSI image sensor device will have a uniform thickness when etched using the thus obtained etching solution.