Patents by Inventor Kevin Shields
Kevin Shields has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11850592Abstract: A cell sorting device is disclosed, wherein the device includes a sorting magnet and at least one particle manipulation device, wherein the particle manipulation device is formed on a surface of a fabrication substrate. The device may include at least one fluid channel, wherein the sorting magnet and the particle manipulation device are in fluid communication with one another through at least one fluid channel. A method of sorting cells from a first cell suspension is also disclosed, The method may include a) magnetic labeling of first target cells and removal of the non-target cells by applying magnetic fields to obtain a second cell suspension; b) fluorescence-activated labeling of second target cells present in the second cell suspension and separating the fluorescence-activated second target cells from the not labeled cells to obtain a third cell suspension.Type: GrantFiled: February 11, 2020Date of Patent: December 26, 2023Assignee: Owl biomedical, Inc.Inventors: John S. Foster, Kevin Shields, Mehran Hoonejani
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Publication number: 20230124069Abstract: A MEMS-based particle manipulation system which uses a particle manipulation stage and a plurality of laser interrogation regions. The laser interrogation regions may be used to assess the effectiveness or accuracy of the particle manipulation stage. In one exemplary embodiment, the particle manipulation stage is a microfabricated, flap-type fluid valve, which sorts a target particle from non-target particles in a fluid stream. The laser interrogation stages are disposed in the microfabricated fluid channels at the input and output of the flap-type sorting valve. The laser interrogation regions may be used to assess the effectiveness or accuracy of the sorting, and to control or adjust sort parameters during the sorting process. One or more feedback loops may be used to improve the particle manipulation process, based on data acquired during the first interrogation and/or during a downstream confirmation. Artificial intelligence techniques may be used to good effect.Type: ApplicationFiled: October 14, 2021Publication date: April 20, 2023Inventors: Daryl GRUMMITT, Mehran Hoonejani, Kevin SHIELDS, John HARLEY, Matthew MASON, Mark NAIVAR
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Publication number: 20220362778Abstract: A microfabricated droplet dispensing structure is described, which may include a MEMS microfluidic fluidic valve, configured to open and close a microfluidic channel. The opening and closing of the valve may separate a target biological particle containing genomic material, and a bead from a sample stream, and direct these two particle into a single droplet formed at the edge of the substrate. The droplet may then be encased in a sheath flow of an immiscible fluid, and provided to a downstream workflow.Type: ApplicationFiled: July 26, 2022Publication date: November 17, 2022Applicant: Owl biomedical, Inc.Inventors: John S. Foster, Mehran Hoonejani, Kevin Shields, Hansueli Meyer, Robert Pinard, Matthias Wahl, Sasa Lazevski, John Harley
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Patent number: 11449998Abstract: A convolutional neural network (CNN) is applied to identifying tumors in a histological image. The CNN has one channel assigned to each of a plurality of tissue classes that are to be identified, there being at least one class for each of non-tumorous and tumorous tissue types. Multi-stage convolution is performed on image patches extracted from the histological image followed by multi-stage transpose convolution to recover a layer matched in size to the input image patch. The output image patch thus has a one-to-one pixel-to-pixel correspondence with the input image patch such that each pixel in the output image patch has assigned to it one of the multiple available classes. The output image patches are then assembled into a probability map that can be co-rendered with the histological image either alongside it or over it as an overlay. The probability map can then be stored linked to the histological image.Type: GrantFiled: August 6, 2020Date of Patent: September 20, 2022Assignee: Leica Biosystems Imaging, Inc.Inventors: Walter Georgescu, Allen Olson, Bharat Annaldas, Darragh Lawler, Kevin Shields, Kiran Saligrama, Mark Gregson
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Publication number: 20220288590Abstract: A microfabricated particle manipulation system, wherein a target particle is pierced by a microfabricated actuator or by a microfabricated knife edge. In either case, the particle membrane is altered, so as to allow material to traverse the membrane. The device may be used to extract cellular material from inside a cell, or to transfect a cell with foreign material.Type: ApplicationFiled: June 3, 2022Publication date: September 15, 2022Applicant: Owl biomedical, Inc.Inventors: John S. FOSTER, Mehran Hoonejani, Kevin SHIELDS, Lily Li
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Publication number: 20210268506Abstract: A microfabricated droplet dispensing structure is described, which may include a MEMS microfluidic fluidic valve, configured to open and close a microfluidic channel. The opening and closing of the valve may separate a target biological particle containing genomic material, and a bead from a sample stream, and direct these two particle into a single droplet formed at the edge of the substrate. The droplet may then be encased in a sheath flow of an immiscible fluid, and provided to a sequencing module. The sequencing module may sequence the genomic material and/or an identifying barcode attached to the bead.Type: ApplicationFiled: May 19, 2021Publication date: September 2, 2021Applicant: Owl biomedical, Inc.Inventors: John S FOSTER, Mehran Hoonejani, Kevin SHIELDS, Hansueli Meyer, Robert Pinard, Matthias Wahl
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Patent number: 11053056Abstract: Glue bottle anti-clog caps are provided. The anti-clog cap has a body with a mounting section for attaching the body to a glue bottle. The body of the anti-clog cap has a dispensing section that includes a central lumen extending from the second mounting end. The body of the anti-clog cap also has a tip section with a support rib protruding from an outlet of the dispensing section. The tip has a tip plug protruding from the support rib. The anti-clog cap has a nozzle that mates to the body and a tip orifice aligned with the tip plug of the body. The tip section is configured to allow the free flow of glue through the cap. The nozzle can rotate between an open and closed position. In the closed position the tip plug can extend into the tip orifice thereby preventing the flow of glue.Type: GrantFiled: April 13, 2018Date of Patent: July 6, 2021Assignee: Sanford, L.P.Inventors: Craig P. E. Davidson, Ryan Heiser, Joseph Wayne Kreft, Patrick Kevin Shields
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Patent number: 11040347Abstract: A microfabricated droplet dispensing structure is described, which may include a MEMS microfluidic fluidic valve, configured to open and close a microfluidic channel. The opening and closing of the valve may separate a target particle and a bead from a sample stream, and direct these two particle into a single droplet formed at the edge of the substrate. The droplet may then be encased in a sheath flow of an immiscible fluid.Type: GrantFiled: June 14, 2018Date of Patent: June 22, 2021Assignee: Owl biomedical, Inc.Inventors: John S Foster, Mehran Hoonejani, Kevin Shields
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Publication number: 20200364867Abstract: A convolutional neural network (CNN) is applied to identifying tumors in a histological image. The CNN has one channel assigned to each of a plurality of tissue classes that are to be identified, there being at least one class for each of non-tumorous and tumorous tissue types. Multi-stage convolution is performed on image patches extracted from the histological image followed by multi-stage transpose convolution to recover a layer matched in size to the input image patch. The output image patch thus has a one-to-one pixel-to-pixel correspondence with the input image patch such that each pixel in the output image patch has assigned to it one of the multiple available classes. The output image patches are then assembled into a probability map that can be co-rendered with the histological image either alongside it or over it as an overlay. The probability map can then be stored linked to the histological image.Type: ApplicationFiled: August 6, 2020Publication date: November 19, 2020Inventors: Walter GEORGESCU, Allen OLSON, Bharat ANNALDAS, Darragh LAWLER, Kevin SHIELDS, Kiran SALIGRAMA, Mark GREGSON
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Patent number: 10740896Abstract: A convolutional neural network (CNN) is applied to identifying tumors in a histological image. The CNN has one channel assigned to each of a plurality of tissue classes that are to be identified, there being at least one class for each of non-tumorous and tumorous tissue types. Multi-stage convolution is performed on image patches extracted from the histological image followed by multi-stage transpose convolution to recover a layer matched in size to the input image patch. The output image patch thus has a one-to-one pixel-to-pixel correspondence with the input image patch such that each pixel in the output image patch has assigned to it one of the multiple available classes. The output image patches are then assembled into a probability map that can be co-rendered with the histological image either alongside it or over it as an overlay. The probability map can then be stored linked to the histological image.Type: GrantFiled: December 21, 2018Date of Patent: August 11, 2020Assignee: LEICA BIOSYSTEMS IMAGING, INC.Inventors: Walter Georgescu, Allen Olson, Bharat Annaldas, Darragh Lawler, Kevin Shields, Kiran Saligrama, Mark Gregson
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Patent number: 10737269Abstract: A particle manipulation system uses a MEMS-based, microfabricated particle manipulation device which has a sample inlet channel, output channels, and a movable member formed on a substrate. The device may be used to separate a target particle from non-target material in a sample stream. In order to improve the sorter speed, accuracy or yield, the particle manipulation system may also include a microfluidic structure which focuses the target particles in a particular portion of the sample inlet channel. The particle manipulation device may have two separate sort output channels, wherein the sort channel used depends on the characteristics of the sort pulse delivered to the micromechanical particle manipulation device.Type: GrantFiled: November 13, 2017Date of Patent: August 11, 2020Assignee: Owl biomedical, Inc.Inventors: John S. Foster, Kevin Shields, Mehran Hoonejani
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Publication number: 20200179931Abstract: A cell sorting device is disclosed, wherein the device includes a sorting magnet and at least one particle manipulation device, wherein the particle manipulation device is formed on a surface of a fabrication substrate. The device may include at least one fluid channel, wherein the sorting magnet and the particle manipulation device are in fluid communication with one another through at least one fluid channel. A method of sorting cells from a first cell suspension is also disclosed, The method may include a) magnetic labeling of first target cells and removal of the non-target cells by applying magnetic fields to obtain a second cell suspension; b) fluorescence-activated labeling of second target cells present in the second cell suspension and separating the fluorescence-activated second target cells from the not labeled cells to obtain a third cell suspension.Type: ApplicationFiled: February 11, 2020Publication date: June 11, 2020Applicant: Owl biomedical, Inc.Inventors: John S. FOSTER, Kevin SHIELDS, Mehran Hoonejani
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Publication number: 20200156830Abstract: Glue bottle anti-clog caps are provided. The anti-clog cap has a body with a mounting section for attaching the body to a glue bottle. The body of the anti-clog cap has a dispensing section that includes a central lumen extending from the second mounting end. The body of the anti-clog cap also has a tip section with a support rib protruding from an outlet of the dispensing section. The tip has a tip plug protruding from the support rib. The anti-clog cap has a nozzle that mates to the body and a tip orifice aligned with the tip plug of the body. The tip section is configured to allow the free flow of glue through the cap. The nozzle can rotate between an open and closed position. In the closed position the tip plug can extend into the tip orifice thereby preventing the flow of glue.Type: ApplicationFiled: April 13, 2018Publication date: May 21, 2020Applicant: Sanford, L.P.Inventors: Craig P.E. DAVIDSON, Ryan HEISER, Joseph Wayne KREFT, Patrick Kevin SHIELDS
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Publication number: 20190381506Abstract: A microfabricated droplet dispensing structure is described, which may include a MEMS microfluidic fluidic valve, configured to open and close a microfluidic channel. The opening and closing of the valve may separate a target particle and a bead from a sample stream, and direct these two particle into a single droplet formed at the edge of the substrate. The droplet may then be encased in a sheath flow of an immiscible fluid.Type: ApplicationFiled: June 14, 2018Publication date: December 19, 2019Applicant: Owl biomedical, Inc.Inventors: John S FOSTER, Mehran Hoonejani, Kevin SHIELDS
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Publication number: 20190292511Abstract: A microfabricated particle manipulation system, wherein a target particle is pierced by a microfabricated actuator or by a microfabricated knife edge. In either case, the particle membrane is altered, so as to allow material to traverse the membrane. The device may be used to extract cellular material from inside a cell, or to transfect a cell with foreign material.Type: ApplicationFiled: May 25, 2018Publication date: September 26, 2019Applicant: Owl biomedical, Inc.Inventors: John S. FOSTER, Mehran Hoonejani, Kevin SHIELDS, Lily Li
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Patent number: D895438Type: GrantFiled: January 23, 2019Date of Patent: September 8, 2020Assignee: Sanford, L.P.Inventors: Ryan Heiser, Patrick Kevin Shields, Michael Desjardins
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Patent number: D915822Type: GrantFiled: October 4, 2019Date of Patent: April 13, 2021Assignee: The Coleman Company, Inc.Inventor: Patrick Kevin Shields
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Patent number: D924001Type: GrantFiled: October 4, 2019Date of Patent: July 6, 2021Assignee: The Coleman Company, Inc.Inventor: Patrick Kevin Shields
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Patent number: D947038Type: GrantFiled: September 4, 2020Date of Patent: March 29, 2022Assignee: Sanford, L.P.Inventors: Ryan Heiser, Patrick Kevin Shields, Michael Desjardins
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Patent number: D970967Type: GrantFiled: October 4, 2019Date of Patent: November 29, 2022Assignee: The Coleman Company, Inc.Inventor: Patrick Kevin Shields