Abstract: The present invention provides a cannula for delivering a tissue graft to a treatment site. The cannula comprises a distal end, a proximal end, a bore formed by an interior surface of the cannula, extending from the distal end to the proximal end and having a diameter, and a tissue graft inside the bore and in contact with the interior surface. The tissue graft may be released from the cannula at the distal end to the treatment site. The tissue graft may have a final loading pressure of 20-200 psi. Also provided is a delivery system comprising the cannula and a delivery device. Further provided are methods for delivering a tissue graft in a cannula to a treatment site and methods for preparing a cannula loaded with the tissue graft.