Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.

Abstract: A syringe assembly includes a barrel that has a first end and a second end. Each of the first and second ends is open. A needle hub is fluidly coupled to and extends away from the second end. A plunger extends into the first end and includes an internal end positioned within the barrel and an external end positioned distal to the barrel. A longitudinal axis is defined extending through the external end and the second end. A needle is fluidly coupled to and extends away from the needle hub. A grip is attached to the plunger. The grip is engaged by a person's finger to urge the plunger into the barrel. The grip has an outward portion extending outwardly away from the plunger and away from the longitudinal axis. The outward portion extends generally toward the barrel.