Abstract: The present disclosure is directed to methods for evaluating system inertness, such as the inertness of a LC or other fluidic system. Some methods are directed to tests wherein the column has been removed prior to injecting a sample including a positive (e.g., metal reacting moiety) control into the system. Some methods can include: (1) repeatedly injecting the sample into a system, the system comprising: fluidic paths wherein interior surfaces of the fluidic paths define wetted surfaces, and wherein at least a portion of the wetted surfaces of the fluidic flow path are coated with an inert coating, wherein the inert coating is inert to at least one analyte in the sample; (2) detecting a value associated with the positive control; and (3) analyzing values associated with the detected positive control to determine system inertness.

Abstract: The present disclosure is directed to surface modified materials such as stationary phase materials for performing size exclusion chromatography. Aspects of the present disclosure feature materials surface modified with a moiety including a polyethylene glycol (PEG) functionality and a moiety comprising a diol functionality. Such surface modified materials exhibit a reduced propensity for ionic and hydrophobic secondary interactions.

Abstract: The present invention relates to a method for separating a sample comprising a nucleic acid, the method comprising: (a) loading a sample comprising a nucleic acid onto an anion-exchange column; and (b) eluting the sample from the anion-exchange column using a salt gradient, wherein a mobile phase used in the eluting step comprises a mild ion-pairing cation, and wherein a column temperature of the anion-exchange column is greater than 30° C. The present invention also relates to a method for determining one or more calorimetric properties of a nucleic acid using anion-exchange chromatography.

Abstract: In various embodiments, the present disclosure pertains to organic polymer core-shell particles that comprise a non-porous organic polymer core (i.e., having a pore volume of less than 0.1 cc/g) and a porous organic polymer shell (i.e., having a pore volume of greater than 0.1 cc/g), in which the porous organic polymer shell has a pore size ranging from 100 ? to 3000 ?. In some embodiments, the present disclosure pertains to chromatographic separation devices that comprise such organic polymer core-shell particles. In some embodiments, the present disclosure pertains to chromatographic methods that comprise: (a) loading a sample onto a chromatographic column comprising such organic polymer core-shell particles and (b) flowing a mobile phase through the column.

Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

Abstract: The present disclosure relates to methods and workflows for processing and analyzing clinical samples (e.g., nasal swabs, throat swabs, plasma samples, urine, etc.) In general, the methods and workflows involve the detection of an infectious state and are improved over contemporary methods due to increased throughput with improved resolution of positive results.

Abstract: The present disclosure is directed to a kit for evaluating system inertness. The kit includes a positive control comprising a metal interacting moiety, and a negative control that does not contain a metal interacting moiety. In some embodiments the kit also includes a container to hold a system suitability solution (e.g., an equimolar mixture of the positive control and the negative control).

Abstract: The present disclosure relates to a method of reducing degradation of sample components in a liquid chromatography system. The method utilizes a masked metal frit to prevent or reduce metal surfaces from becoming catalytically active. The masked metal frit is a metal based frit that includes a coating on its exterior surfaces to mask or prevent contact between the organic solvents (and/or any analyte or other related solvent) and the underlying metal. The coating is a vapor deposited inorganic-organic hybrid coating, such as a vapor deposited C2 coating.

Abstract: The present disclosure is directed to methods for evaluating system inertness, such as the inertness of a LC or other fluidic system. Some methods are directed to tests wherein the column has been removed prior to injecting a sample including a positive (e.g., metal reacting moiety) control into the system. Some methods can include: (1) repeatedly injecting the sample into a system, the system comprising: fluidic paths wherein interior surfaces of the fluidic paths define wetted surfaces, and wherein at least a portion of the wetted surfaces of the fluidic flow path are coated with an inert coating, wherein the inert coating is inert to at least one analyte in the sample; (2) detecting a value associated with the positive control; and (3) analyzing values associated with the detected positive control to determine system inertness.

Abstract: The present disclosure is directed to methods of characterizing a system containing a chromatographic column. The methods can include introducing a sample comprising a positive control and a negative control to the system containing a chromatographic column, wherein the positive control is a sensitive probe that interacts with the system and the negative control is substantially non-interacting with the system; after passing the sample through the chromatographic column, detecting the positive control and the negative control; and determining system suitability by comparing the amount of detected positive control to negative control. In some embodiments, determining system suitability (e.g., inertness of sample to the system) is accomplished by determining a ratio of detected positive control to negative control.

Abstract: The present disclosure relates to the use of vapor deposition coated flow paths for improved chromatography and sample analysis using liquid chromatography-mass spectrometry (LC/MS) or liquid chromatography-optical detection (LC/UV). More specifically, this technology relates to separating and quantitation of analytes (e.g., phospho prodrugs and its phosphorylated metabolites) from a sample matrix (e.g., mammalian blood, plasma) using chromatographic devices and fluidic systems having coated flow paths. The LC-MS or LC-UV techniques provide improved recovery, peak shape and dynamic range in the analysis of the prodrug and its metabolites.

Abstract: The present invention provides porous materials for use in solid phase extractions and chromatography. In particular, the materials exhibit superior properties in the SPE analysis of biological materials. In certain aspects, the porous materials comprise a copolymer of a least one hydrophobic monomer and at least one hydrophilic monomer, wherein more than 10% of the BJH surface area of the porous material is contributed by pores that have a diameter greater than or equal to 200 ?, wherein said material has a median pore diameter of about 100 ? to about 1000 ?, or both. In some embodiments, the at least one hydrophilic monomer has a log P value of less than 0.5. In some embodiments, the at least one hydrophilic monomer is selected from 4-acryloymorphine, N-(3-methoxypropyl)acrylamide, N,N?-methylenebis(acrylamide), acrylonitrile, ethylene glycol dimethacrylate, methyl acrylate, 4-acetoxystyrene, 4-vinyl pyridine, or a boronic-acid-containing monomer, among others.

Abstract: A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of a vapor deposition coated frit together with an uncoated frit in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having a single coated frit within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.

Abstract: A chromatographic device including a coated metallic frit is disclosed. The coating is provided over the fluid exposed surfaces of the frit, thereby covering metallic surfaces to prevent interaction with an analyte in the flowstream. This technology relates to the use of vapor deposition coated frit(s) in a liquid flow path for improved chromatography. More specifically, this technology relates to liquid chromatographic devices for separating analytes in a sample having coated frit(s) within an uncoated metallic fluidic flow path (i.e., the column tube or channel is formed of stainless steel, titanium (pure or alloyed), or some mixture of stainless steel and titanium) and does not include the coating applied to the frit.

Abstract: The present disclosure generally relates to systems, methods and devices for providing pressurized solvent flow in chromatography systems.

Abstract: The present invention is directed to a method for performing size exclusion chromatography. Embodiments of the present invention feature devices and methods for improving the speed and separations of size exclusion chromatography using a stationary phase material comprising small particles (<2 micron in diameter).

Abstract: The present disclosure generally relates to systems, methods and devices for providing pressurized solvent flow in chromatography systems.

Abstract: Novel material for chromatographic separations, processes for its preparation, and separations devices containing the chromatographic material. In particular, the disclosure describes porous inorganic/organic hybrid particles having a chromatographically-enhancing pore geometry, which desirably may be surface modified, and that offer more efficient chromatographic separations than that known in the art.

Abstract: Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, numerous equivalents to the specific procedures described herein. Such equivalents were considered to be within the scope of this invention and are covered by the following claims. The contents of all references, issued patents, and published patent applications cited throughout this application are hereby incorporated by reference.