Patents by Inventor Khairuzzaman B. Mullah
Khairuzzaman B. Mullah has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7166715Abstract: The invention provides an asymmetric cyanine dye compound having the structure including substituted forms thereof, wherein, at least one of R1 and R2 is linking group, X is O, S, or Se, and n ranges from 0 to 2. The invention further provides reporter-quencher dye pairs comprising the asymmetric cyanine dyes, dye-labelled polynucleotides incorporating the asymmetric cyanine dyes, and hybridization detection methods utilizing the dye-labelled polynucleotides.Type: GrantFiled: May 5, 2004Date of Patent: January 23, 2007Assignee: Applera CorporationInventors: Linda G. Lee, Ronald J. Graham, Khairuzzaman B. Mullah, Francis T. Haxo
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Patent number: 6835827Abstract: Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure where S is a solid-support, A is a cleavable linker, X is a moiety with three or more attachment sites, L is a label, Y is a nucleophile, i.e. O, NH, NR or S, and P1 is an acid cleavable protecting group are provided. The labelled solid-support is reacted in a cyclical fashion to synthesize a labelled oligonucleotide on a solid-support in the 5′ to 3′ direction, having the structure: Labelled oligonucleotides are also synthesized by reacting: (i) a label reagent bearing functionality consisting of carboxylic acid, sulfonic acid, phosphonic acid, or phosphoric acid, (ii) an oligonucleotide on solid support with nucleophilic functionality, and (iii) a coupling reagent, whereby an ester, amide, thioester, sulfonamide, sulfonate, phosphonate, phosphoramidate, phosphorothioate, or phosphate bond is formed.Type: GrantFiled: February 20, 2003Date of Patent: December 28, 2004Assignee: Applera CorporationInventors: Ravi S. Vinayak, Linda G. Lee, Khairuzzaman B. Mullah, Barnett B. Rosenblum
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Patent number: 6750024Abstract: The invention provides an asymmetric cyanine dye compound having the structure including substituted forms thereof, wherein, at least one of R1 and R2 is linking group, X is O, S, or Se, and n ranges from 0 to 2. The invention further provides reporter-quencher dye pairs comprising the asymmetric cyanine dyes, dye-labelled polynucleotides incorporating the asymmetric cyanine dyes, and hybridization detection methods utilizing the dye-labelled polynucleotides.Type: GrantFiled: March 31, 2003Date of Patent: June 15, 2004Assignee: Applera CorporationInventors: Linda G. Lee, Ronald J. Graham, Khairuzzaman B. Mullah, Francis T. Haxo
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Publication number: 20030207305Abstract: The invention provides an asymmetric cyanine dye compound having the structure 1Type: ApplicationFiled: March 31, 2003Publication date: November 6, 2003Applicant: Applera CorporationInventors: Linda G. Lee, Ronald J. Graham, Khairuzzaman B. Mullah, Francis T. Haxo
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Publication number: 20030191303Abstract: Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure 1Type: ApplicationFiled: February 20, 2003Publication date: October 9, 2003Applicant: Applera CorporationInventors: Ravi S. Vinayak, Linda G. Lee, Khairuzzaman B. Mullah, Barnett B. Rosenblum
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Patent number: 6541618Abstract: The invention provides an asymmetric cyanine dye compound having the structure including substituted forms thereof, wherein, at least one of R1 and R2 is linking group, X is O, S, or Se, and n ranges from 0 to 2. The invention further provides reporter-quencher dye pairs comprising the asymmetric cyanine dyes, dye-labelled polynucleotides incorporating the asymmetric cyanine dyes, and hybridization detection methods utilizing the dye-labelled polynucleotides.Type: GrantFiled: June 21, 2000Date of Patent: April 1, 2003Assignee: Applera CorporationInventors: Linda G. Lee, Ronald J. Graham, Khairuzzaman B. Mullah, Francis T. Haxo
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Patent number: 6525183Abstract: Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure where S is a solid-support, A is a cleavable linker, X is a moiety with three or more attachment sites, L is a label, Y is a nucleophile, i.e. O, NH, NR or S, and P1 is an acid cleavable protecting group are provided. The labelled solid-support is reacted in a cyclical fashion to synthesize a labelled oligonucleotide on a solid-support in the 5′ to 3′ direction, having the structure: Labelled oligonucleotides are also synthesized by reacting: (i) a label reagent bearing functionality consisting of carboxylic acid, sulfonic acid, phosphonic acid, or phosphoric acid, (ii) an oligonucleotide on solid support with nucleophilic functionality, and (iii) a coupling reagent, whereby an ester, amide, thioester, sulfonamide, sulfonate, phosphonate, phosphoramidate, phosphorothioate, or phosphate bond is formed.Type: GrantFiled: November 7, 2001Date of Patent: February 25, 2003Assignee: PE Corporation (NY)Inventors: Ravi S. Vinayak, Linda G. Lee, Khairuzzaman B. Mullah, Barnett B. Rosenblum
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Publication number: 20020165389Abstract: Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure 1Type: ApplicationFiled: November 7, 2001Publication date: November 7, 2002Applicant: PE Corporation (NY)Inventors: Ravi S. Vinayak, Linda G. Lee, Khairuzzaman B. Mullah, Barnett B. Rosenblum
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Patent number: 6348596Abstract: The invention provides asymmetric cyanine dye compounds having the general formula: including substituted forms thereof, which are non-fluorescent quencher molecules. The invention further provides reporter-quencher dye pairs, wherein the asymmetric cyanine dyes are the quenchers, polynucleotides incorporating the asymmetric cyanine dyes, and nucleic acid hybridization detection methods utilizing the dye-labeled polynucleotides.Type: GrantFiled: July 20, 1999Date of Patent: February 19, 2002Assignee: PE Corporation (NY)Inventors: Linda G. Lee, Ronald J. Graham, Khairuzzaman B. Mullah, Francis T. Haxo
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Patent number: 6316610Abstract: Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure where S is a solid-support, A is a cleavable linker, X is a moiety with three or more attachment sites, L is a label, Y is a nucleophile, i.e. O, NH, NR or S, and P1 is an acid cleavable protecting group are provided. The labelled solid-support is reacted in a cyclical fashion to synthesize a labelled oligonucleotide on a solid-support in the 5′ to 3′ direction, having the structure: Labelled oligonucleotides are also synthesized by reacting: (i) a label reagent bearing functionality consisting of carboxylic acid, sulfonic acid, phosphonic acid, or phosphoric acid, (ii) an oligonucleotide on solid support with nucleophilic functionality, and (iii) a coupling reagent, whereby an ester, amide, thioester, sulfonamide, sulfonate, phosphonate, phosphoramidate, phosphorothioate, or phosphate bond is formed.Type: GrantFiled: March 20, 2001Date of Patent: November 13, 2001Assignee: PE Corporation (NY)Inventors: Linda G. Lee, Khairuzzaman B. Mullah, Barnett B. Rosenblum
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Publication number: 20010014735Abstract: Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure 1Type: ApplicationFiled: March 20, 2001Publication date: August 16, 2001Applicant: PE Corporation (NY)Inventors: Linda G. Lee, Khairuzzaman B. Mullah, Barnett B. Rosenblum
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Patent number: 6255476Abstract: Methods and compositions to label oligonucleotides and analogs directly on a solid-support having the structure where S is a solid-support, A is a cleavable linker, X is a moiety with three or more attachment sites, L is a label, Y is a nucleophile, i.e. O, NH, NR or S, and P1 is an acid cleavable protecting group are provided. The labelled solid-support is reacted in a cyclical fashion to synthesize a labelled oligonucleotide on a solid-support in the 5′ to 3′ direction, having the structure: Labelled oligonucleotides are also synthesized by reacting: (i) a label reagent bearing functionality consisting of carboxylic acid, sulfonic acid, phosphonic acid, or phosphoric acid, (ii) an oligonucleotide on solid support with nucleophilic functionality, and (iii) a coupling reagent, whereby an ester, amide, thioester, sulfonamide, sulfonate, phosphonate, phosphoramidate, phosphorothioate, or phosphate bond is formed.Type: GrantFiled: February 22, 1999Date of Patent: July 3, 2001Assignee: PE Corporation (NY)Inventors: Ravi S. Vinayak, Linda G. Lee, Khairuzzaman B. Mullah, Barnett B. Rosenblum
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Patent number: 6080868Abstract: The invention provides an asymmetric cyanine dye compound having the structure ##STR1## including substituted forms thereof, wherein, at least one of R.sub.1 and R.sub.2 is linking group, X is O, S, or Se, and n ranges from 0 to 2. The invention further provides reporter-quencher dye pairs comprising the asymmetric cyanine dyes, dye-labeled polynucleotides incorporating the asymmetric cyanine dyes, and hybridization detection methods utilizing the dye-labeled polynucleotides.Type: GrantFiled: January 23, 1998Date of Patent: June 27, 2000Assignee: The Perkin-Elmer CorporationInventors: Linda G. Lee, Ronald J. Graham, Khairuzzaman B. Mullah, Francis T. Haxo