Abstract: The present disclosure generally relates to devices and procedures for the development of glucose oxidase-bound electrodes by a covalent binding of glucose oxidase on amine-functionalized electrodes. More particularly, the present disclosure is related to covalently-bound enzyme-coated electrodes that are leach-proof and highly stable for continuous glucose monitoring. The glucose oxidase-bound electrodes are employed for the development of a mediator-less electrochemical glucose sensing procedure having no interference from biological substances and drugs.