Abstract: The 3D image generation with position-sensing gamma probe utilizes a gamma probe connected to a control unit. The control unit is also connected to a display unit. A probe contact surface feeds position information to the display unit. The gamma probe is moved across a region of interest over the probe contact surface, generating position-dependent gamma rates with the help of the control unit. The probe contact surface is utilized to determine the position of the probe head and provides position information, which, together with the gamma rate, enables generation of a rate map in the display unit. This allows producing 2D and 3D images of gamma activity concentrations and assures proper recording of scan data. The scanning area of interest is pre-defined by frames having shapes and contours optimized for intended applications. 2D and 3D images can be obtained in a single scanning cycle.