Abstract: The invention relates to antibodies against HER3 and their use in the treatment of cancer.

Abstract: A method for producing a variant of a parent polypeptide including a Fc region, which variant exhibits reduced binding to the protein C1q and to at least one receptor Fcg? R as compared to the parent polypeptide.

Abstract: A variant of a parent polypeptide including an Fc region, which variant exhibits increased binding to FcRn as compared to the parent polypeptide and includes at least one amino acid modification in the Fc region.

Abstract: The invention relates to antibodies against HER3 and their use in the treatment of cancer.

Abstract: A variant of a parent polypeptide including an Fc region, which variant exhibits increased binding to FcRn as compared to the parent polypeptide and includes at least one amino acid modification in the Fc region.

Abstract: The invention concerns a method for random mutagenesis comprising the replication of a DNA sequence in the presence of an efficient amount of at least a mutase, for example a Pol ?, the random mutagenesis rate being at least of the order of 1 mutation for 400 base pairs. The replication product, optionally recombined and amplified, is cloned in an expression vector so as to generate mutated polypeptides which will be selected on the basis of the desired property or properties.

Abstract: The present invention provides an in vitro method for obtaining a library of polynucleotides encoding antibodies, derivatives thereof or fragments thereof, comprising the step of performing random mutagenesis of a polynucleotide encoding the variable region of a heavy chain and/or the variable region of a light chain of a light chain, wherein random mutagenesis is performed on a library of polynucleotides comprising a sequence encoding the variable region of a heavy chain and/or the variable region of a light chain; and wherein the random mutagenesis process creates randomly distributed mutations along at least 70% of the sequence encoding the variable region.

Abstract: This invention relates to an expression cassette for expressing polypeptides in eukaryotic cells using alternative splicing. The expression cassette comprises in 5? to 3? downstream direction: a promoter; a sequence transcribed in a 5? untranslated region (5?UTR); a donor splice site; an intron; a first acceptor splice site; a first cistron encoding a first polypeptide; a second acceptor splice site; a second cistron encoding a second polypeptide; an internal ribosome entry site (IRES) operably linked to a selection marker; and a sequence transcribed in a 3? untranslated region (3?UTR) including a polyadenylation signal, wherein the polyadenylation signal is unique.

Abstract: The present invention provides a versatile and sensitive method for studying interaction of two peptides or polypeptides A and B within the cytoplasm of a host cell. The method is based on the use of two distinct chimeric polypeptides. The first chimeric polypeptide containing an aggregation domain fused to a polypeptide A and the second one containing a phenotype-associated functional domain fused to a second polypeptide B. When these chimeric polypeptides are co-expressed within a host cell allowing aggregation of the aggregation domain, the phenotype of the host cell depends on the entrapment of the phenotype-associated functional domain which only occurs when A and B interact with each other.

Abstract: The invention relates to a gene expression inducing fusion protein comprising a) a ribonucleic acid linking peptide domain and a post-transcriptional gene expression activator domain and b) a domain enabling cytoplasmic membrane delocalization. The invention also relates to a modulatable permanent external method for controlling gene expression induction by modulating the state of post-translational modulation of the gene expression inducing fusion protein for the expression of said gene.

Abstract: The invention concerns biotin derivatives of general formula (I), wherein: R1 represents a specific compound; X1 represents a carbonaceous chain, preferably of 1 to 10 carbon atoms, substituted or not, optionally comprising one or several carbonyl or oxycarbonyl groups, and/or a —NH— group, in particular a chain of formula —HN(CH2)n—O—CO wherein n is an integer between 1 and 5 and optionally comprising one or several ether, amide and/or amino functions; Y represents a hydrocarbon chain of 1 to 5 carbon atoms, substituted or not; R2 represents a hydrogen atom, or a protecting group, in particular R2 represents an alkyl group of 1 to 5 carbon atoms. The invention is useful as vectors, in particular for implementing methods for detecting interactions between biological compounds, and in pharmaceutical compositions.

Abstract: The invention concerns a method for random mutagenesis comprising the replication of a DNA sequence in the presence of an efficient amount of at least a mutase, for example a Pol &bgr;, the random mutagenesis rate being at least of the order of 1 mutation for 400 base pairs. The replication product, optionally recombined and amplified, is cloned in an expression vector so as to generate mutated polypeptides which will be selected on the basis of the desired property or properties.

Abstract: A variant of a parent polypeptide including an Fc region, which variant exhibits increased binding to FcRn as compared to the parent polypeptide and includes at least one amino acid modification in the Fc region.

Abstract: A variant of a parent polypeptide including an Fc region, which variant exhibits increased binding to FcRn as compared to the parent polypeptide and includes at least one amino acid modification in the Fc region.