Abstract: The present invention relates to a biomarker composition for diagnosing and predicting prognosis of prostate cancer, and that the detection rate and expression level of gene combination composed with AR (Androgen receptor), AR-V7 (Androgen receptor variant 7), EpCAM (Epithelial cell adhesion molecule) in blood tumor cells (CTC) isolated from patients, Epithelial cell adhesion molecule), KRT-19 (Cytokeratin 19), PSA (Prostate specific antigen), and PSMA (Prostate specific membrane antigen) in circulating tumor cells (CTCs) isolated from patients is related to the level of malignancy in prostate cancer is confirmed and thus the gene combination provides as a biomarker for prostate cancer diagnosis and a biomarker for prognosis prediction of prostate cancer.

Abstract: Provided are an active droplet generating apparatus capable of controlling a droplet size, a method of controlling a droplet size using the same, and a self-diagnosis apparatus for diagnosing generation of a droplet, the active droplet generating apparatus including: a disposable microchannel upper plate; a multifunctional lower plate separated from the disposable microchannel upper plate and configured to be permanently used separately from the disposable microchannel upper plate; a functional polymeric film provided on a lower surface of the upper plate; a negative pressure forming means; and a flow velocity control device configured to adjust the droplet size to a desired size by receiving, by feedback, the voltage value measured by the droplet measuring electrode and controlling flow velocities of the oil and the sample, thereby controlling the droplet size in a feedback control manner by quickly and accurately measuring the droplet size using a capacitance impedance technique.

Abstract: Provided is a droplet generation chip including an electrode with a variable sensing area, including a disposable panel in which a microfluidic channel is formed; a substrate which is separated from the disposable panel and can be reused; a thin film which separates the disposable panel and the substrate from each other; a negative pressure generation means so as to allow the disposable panel and the substrate to be attached to or detached from each other; and a sensing electrode unit measuring the length of a droplet in a sample passing through the microfluidic channel, the sensing electrode unit including a first application electrode and a second application electrode; a control means for individual on/off control of application signals of the first application electrode and the second application electrode; and a sensing electrode for sensing a signal according to the position of the droplet introduced into the microfluidic channel.

Abstract: A disposable flow velocity measuring device including: a first panel having a flow velocity measuring structure, and having additional micro protrusion patterns formed around the flow velocity measuring structure; a second panel separated from the first panel, and including a fluid channel through which a sample passes; a porous ultra-thin film formed on a portion, at which the first panel and the second panel come in contact with each other, so that the sample passing through the fluid channel does not directly come in contact with the flow velocity measuring structure, thereby separating the first panel and the second panel and removing micro-air bubbles included in the fluid passing through the fluid channel; a non-porous ultra-thin film formed in a partial region of the porous ultra-thin film; and a negative pressure forming unit for applying negative pressure in order to adsorb the first panel and the second panel.

Abstract: An apparatus and method for separating single cells. The apparatus includes: a fluid channel having an upper panel, a lower panel, and a flow path formed therebetween that is configured to convey a sample including a single cell; a single cell measuring unit including first and second electrodes provided on the fluid channel in a predetermined spaced relationship for applying electrical signals to the sample in the flow path, and a detection electrode provided between the first and second electrodes to detect the single cell in the sample in the flow path, such that the single cell measuring unit applies the electrical signals to the sample in the flow path, detects the electrical signals of the sample, and detects whether there is the single cell in the sample; and a single cell separation control device which outputs a single cell separation control signal when the single cell is detected by the detection electrode.

Abstract: A disposable flow velocity measuring device including: a first panel having a flow velocity measuring structure, and having additional micro protrusion patterns formed around the flow velocity measuring structure; a second panel separated from the first panel, and including a fluid channel through which a sample passes; a porous ultra-thin film formed on a portion, at which the first panel and the second panel come in contact with each other, so that the sample passing through the fluid channel does not directly come in contact with the flow velocity measuring structure, thereby separating the first panel and the second panel and removing micro-air bubbles included in the fluid passing through the fluid channel; a non-porous ultra-thin film formed in a partial region of the porous ultra-thin film; and a negative pressure forming means for applying negative pressure in order to adsorb the first panel and the second panel.

Abstract: Provided is a microfluidic device including a lower panel including flow velocity measuring structures for measuring a flow velocity of a fluid; an upper panel separated from the lower panel and including a microfluidic channel through which a sample passes; a thin film provided at a portion where the lower panel and the upper panel adjoin each other in order to prevent the sample passing through the microfluidic channel from coming into direct contact with the flow velocity measuring structures, the thin film being configured to separate the lower panel and the upper panel to enable the lower panel to be repeatedly used multiple times; a specific functional unit configured to perform a specific operation on the sample passing through the microfluidic channel; and a negative pressure forming means configured to suck the lower panel and the upper panel with a negative pressure.

Abstract: Provided is a method for bonding panel of a microfluidic element by using a releasing film including preparing a panel having a pattern for a microfluidic channel formed on one surface thereof, preparing a release film coated with silicon nanoparticles on one or both surfaces thereof, plasma-treating one surface of the panel on which the pattern for the microfluidic channel is formed and the surface of the release film, and bonding the panel and the release film by performing heat treatment at a predetermined temperature range in a state in which the plasma-treated panel and the release film are attached to each other.

Abstract: Provided is an apparatus for measuring a droplet length using a capacitive electrode including: a disposable panel; a board separated from the disposable panel and configured to be reused separately from the disposable panel; a sensing electrode unit patterned on an upper surface of the board and configured to measure a length of a droplet depending on a voltage value measured depending on a capacitance value of the fluid passing through the microfluid channel; a thin film provided on a lower surface of the disposable panel; and a negative pressure forming means configured to apply a negative pressure between the disposable panel and the board so that the disposable panel and the board are attached to or detached from each other.

Abstract: Provided is a device for measuring a microfluid flow velocity, having a structure separable by an ultra-thin film, the device including a first panel including a flow velocity measurement structure configured to measure a flow velocity of a fluid, a second panel configured to be separated from the first panel and including a microfluid channel through which a sample passes, and the ultra-thin film formed in a portion where the first panel and the second panel adjoin each other, the ultra-thin film being configured to separate the first panel and the second panel so that the sample passing through the microfluid channel does not come into direct contact with the flow velocity measurement structure, in which the first panel including the flow velocity measurement structure is usable multiple times repeatedly.

Abstract: Provided is a method for bonding panel of a microfluidic element by using a releasing film including preparing a panel having a pattern for a microfluidic channel formed on one surface thereof, preparing a release film coated with silicon nanoparticles on one or both surfaces thereof, plasma-treating one surface of the panel on which the pattern for the microfluidic channel is formed and the surface of the release film, and bonding the panel and the release film by performing heat treatment at a predetermined temperature range in a state in which the plasma-treated panel and the release film are attached to each other.

Abstract: Provided are an active droplet generating apparatus capable of controlling a droplet size, a method of controlling a droplet size using the same, and a self-diagnosis apparatus for diagnosing generation of a droplet, the active droplet generating apparatus including: a disposable microchannel upper plate; a multifunctional lower plate separated from the disposable microchannel upper plate and configured to be permanently used separately from the disposable microchannel upper plate; a functional polymeric film provided on a lower surface of the upper plate; a negative pressure forming means; and a flow velocity control device configured to adjust the droplet size to a desired size by receiving, by feedback, the voltage value measured by the droplet measuring electrode and controlling flow velocities of the oil and the sample, thereby controlling the droplet size in a feedback control manner by quickly and accurately measuring the droplet size using a capacitance impedance technique.

Abstract: Provided is a microfluidic device capable of removing microbubbles in a channel by using a porous thin film, the microfluidic device comprising: an upper panel comprising a microfluidic channel through which a fluid passes; a porous thin film attached to the bottom surface of the microfluidic channel so as to remove microbubbles included in the fluid that passes through the microfluidic channel; a lower panel contacting the bottom surface of the porous thin film and the upper panel, a path being provided in the lower panel so as to discharge microbubbles, which pass through the porous thin film, to the outside; and a vacuum-suctioning means for vacuum-suctioning the upper panel and the lower panel such that the microfluidic channel, to which the porous thin film is attached, is attached to the lower panel in a vacuum state.

Abstract: Provided is an apparatus for measuring a droplet length using a capacitive electrode including: a disposable panel; a board separated from the disposable panel and configured to be reused separately from the disposable panel; a sensing electrode unit patterned on an upper surface of the board and configured to measure a length of a droplet depending on a voltage value measured depending on a capacitance value of the fluid passing through the microfluid channel; a thin film provided on a lower surface of the disposable panel; and a negative pressure forming means configured to apply a negative pressure between the disposable panel and the board so that the disposable panel and the board are attached to or detached from each other.

Abstract: The present invention relates to a biomarker composition for diagnosing and predicting prognosis of prostate cancer, and that the detection rate and expression level of gene combination composed with AR (Androgen receptor), AR-V7 (Androgen receptor variant 7), EpCAM (Epithelial cell adhesion molecule) in blood tumor cells (CTC) isolated from patients, Epithelial cell adhesion molecule), KRT-19 (Cytokeratin 19), PSA (Prostate specific antigen), and PSMA (Prostate specific membrane antigen) in circulating tumor cells (CTCs) isolated from patients is related to the level of malignancy in prostate cancer is confirmed and thus the gene combination provides as a biomarker for prostate cancer diagnosis and a biomarker for prognosis prediction of prostate cancer.

Abstract: Provided is a microfluidic device including a lower panel including flow velocity measuring structures for measuring a flow velocity of a fluid; an upper panel separated from the lower panel and including a microfluidic channel through which a sample passes; a thin film provided at a portion where the lower panel and the upper panel adjoin each other in order to prevent the sample passing through the microfluidic channel from coming into direct contact with the flow velocity measuring structures, the thin film being configured to separate the lower panel and the upper panel to enable the lower panel to be repeatedly used multiple times; a specific functional unit configured to perform a specific operation on the sample passing through the microfluidic channel; and a negative pressure forming means configured to suck the lower panel and the upper panel with a negative pressure.

Abstract: Provided is a disposable flow velocity measuring device comprising: a first panel having a flow velocity measuring structure, and having additional micro protrusion patterns formed around the flow velocity measuring structure; a second panel separated from the first panel, and including a fluid channel through which a sample passes; a porous ultra-thin film formed on a portion, at which the first panel and the second panel come in contact with each other, so that the sample passing through the fluid channel does not directly come in contact with the flow velocity measuring structure, thereby separating the first panel and the second panel and removing micro-air bubbles included in the fluid passing through the fluid channel; a non-porous ultra-thin film formed in a partial region of the porous ultra-thin film; and a negative pressure forming means for applying negative pressure in order to adsorb the first panel and the second panel.

Abstract: Provided is a microfluidic device capable of removing microbubbles in a channel by using a porous thin film, the microfluidic device comprising: an upper panel comprising a microfluidic channel through which a fluid passes; a porous thin film attached to the bottom surface of the microfluidic channel so as to remove microbubbles included in the fluid that passes through the microfluidic channel; a lower panel contacting the bottom surface of the porous thin film and the upper panel, a path being provided in the lower panel so as to discharge microbubbles, which pass through the porous thin film, to the outside; and a vacuum-suctioning means for vacuum-suctioning the upper panel and the lower panel such that the microfluidic channel, to which the porous thin film is attached, is attached to the lower panel in a vacuum state.

Abstract: Provided is a device for measuring a microfluid flow velocity, having a structure separable by an ultra-thin film, the device including a first panel including a flow velocity measurement structure configured to measure a flow velocity of a fluid, a second panel configured to be separated from the first panel and including a microfluid channel through which a sample passes, and the ultra-thin film formed in a portion where the first panel and the second panel adjoin each other, the ultra-thin film being configured to separate the first panel and the second panel so that the sample passing through the microfluid channel does not come into direct contact with the flow velocity measurement structure, in which the first panel including the flow velocity measurement structure is usable multiple times repeatedly.

Abstract: The present invention relates to an apparatus and a method for separating single cells, and the apparatus includes: a fluid channel which has an upper panel and a lower panel and has a flow path which is formed between the upper panel and the lower panel and configured to convey a sample including a single cell; a single cell measuring unit which includes first and second electrodes which are provided on the fluid channel so as to be spaced apart from each other at a predetermined interval and apply electrical signals to the sample being conveyed through the flow path of the fluid channel, and a detection electrode which is provided between the first electrode and the second electrode and detects the single cell in the sample being conveyed through the flow path, such that the single cell measuring unit applies the electrical signals to the sample being conveyed through the flow path, detects the electrical signals of the sample to which the electrical signals are applied, and detects whether there is the sin